Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
권호 표지

Anti-inflammatory Activity of Herbal Extracts through Inhibition of TNF-α, IL-6 and IL-8

TNF-α, IL-6, IL-8 저해를 통한 천연약용식물 추출물의 항염증 특성

  • Kim, Yong-Soo (Radiation Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Gwon, Hui-Jeong (Radiation Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Jong-Seok (Radiation Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lim, Youn-Mook (Radiation Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Nho, Young-Chang (Radiation Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 김용수 (한국원자력연구원 정읍방사선과학연구소 방사선공업환경연구부) ;
  • 권희정 (한국원자력연구원 정읍방사선과학연구소 방사선공업환경연구부) ;
  • 박종석 (한국원자력연구원 정읍방사선과학연구소 방사선공업환경연구부) ;
  • 임윤묵 (한국원자력연구원 정읍방사선과학연구소 방사선공업환경연구부) ;
  • 노영창 (한국원자력연구원 정읍방사선과학연구소 방사선공업환경연구부)
  • Received : 2011.09.07
  • Accepted : 2011.09.16
  • Published : 2011.09.30

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Abstract

This study evaluated the cytotoxicity and anti-inflammatory activities of natural herbal extracts including Houttuynia cordata and Eucommia ulmoides against human mast cell (HMC-1). Houttuynia cordata (HC) and Eucommia ulmoides(EU) were extracted with distilled water (at $75^{\circ}C$) and then freeze-dried for 5 days. Finally, the mixture of HC and EU were sterilized by ${\gamma}$-rays irradiation. Cytotoxicity of the mixture against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition, inflammatory mediator cytokines such as TNF-${\alpha}$, IL-6 and IL-8 were evaluated by ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate (PMA). The results showed that mixture of HC and EU had no cytoxicity and reduced TNF-${\alpha}$, IL-6, IL-8 response on HMC-1 cells.

Keywords