생명과학회지 (Journal of Life Science)

  • 제21권8호
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  • Pages.1190-1198
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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추출방법에 따른 한약재의 인체신경모세포 SK-N-SH 보호 효과

Effects of Extraction Methods of Medicinal Plants on Human Growth of Neuroblastoma SK-N-SH Cells

  • 권정민 (경상대학교 응용생명과학부) ;
  • 문연규 (경상대학교 응용생명과학부) ;
  • 김영숙 (경상대학교 농업생명과학연구원) ;
  • 정지영 (경상대학교 농업생명과학연구원) ;
  • 하영래 (경상대학교 응용생명과학부) ;
  • 양재경 (경상대학교 농업생명과학연구원)
  • Kwon, Jung-Min (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University) ;
  • Moon, Yeon-Gyu (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University) ;
  • Kim, Young-Suk (Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Jung, Ji-Young (Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Ha, Yeong-Lae (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University) ;
  • Yang, Jae-Kyung (Institute of Agriculture & Life Sciences, Gyeongsang National University)
  • 투고 : 2011.07.07
  • 심사 : 2011.08.26
  • 발행 : 2011.08.30
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초록

한약재의 추출방법에 따른 인체신경모세포 SK-N-SH에 대한 보호 효과를 연구하였다. 당귀, 건지황, 작약 및 천궁을 시료로 사용하였고, 열수추출(환류냉각, 5 시간), 증숙추출(100$^{\circ}C$ 및 120$^{\circ}C$, 90분 후 열수추출) 방법과 에탄올추출(환류냉각, 5 시간)방법을 비교하였다. 추출물을 농도별로 SK-N-SH 세포에 2 시간 처리한 후 $H_2O_2$로 250${\mu}M$로 2 시간 산화스트레스를 유발한 다음 세포독성 및 apoptosis와 caspase-3의 발현 정도를 측정하였다. 모든 약재의 열수추출물이 다른 추출물보다 세포 증식을 촉진하였고, apoptosis를 억제하였다. 한약재 열수추출물 1${\mu}g/{\mu}l}$ 농도까지는 세포증식을 촉진하였지만, 그 이상의 농도에서는 오히려 감소시켰다. 열수추출물은 다른 추출물보다 총페놀성 화합물이 많이 함유되어 있었고, 항산화능이 높았다. 또한, 이와 같은 효과는 당귀의 열수추출물이 다른 약재 열수추출물보다 우수하였다. 본 연구결과는 약재의 열수추출법이 인체신경모세포인 SK-N-SH의 증식과 세포사멸 억제를 위해 가장 우수한 방법이었고, 당귀 열수추출물이 가장 우수한 효과를 지니고 있어 기억력 보호나 상실억제제로 활용할 수 있을 것이다.

Extraction methods of medicinal plants were evaluated for growth enhancing effects of human neuroblastoma SK-N-SH cells. Hot-water extraction (reflux for 5 hr), hot-water extraction post treatment (100$^{\circ}C$ or 120$^{\circ}C$, 90 min) and ethanol extraction (reflux for 5 hr) methods were applied to Angelica gigas, Rhemania glutinosa, Paeonia lactiflora and Cnidium officinale samples to extract their constituents. Cells were treated for 2 hr with various concentrations of extracts (0, 0.5, 1, 2, 4 ${\mu}g/{\mu}l}$ media) prior to $H_2O_2$ (250 ${\mu}M$) treatment for 2 hr to provide oxidative stress. Cell viability, caspase-3 expression and apoptosis were measured for cells treated with sample extracts. Hot-water extract exhibited a stronger growth enhancing and apoptosis protecting ability than other extracts. These activities were shown at less than 1 ${\mu}g/{\mu}l}$ concentration, and not greater than 2 ${\mu}g/{\mu}l}$ concentration. Hot-water extract contained more polyphenolic compounds than other extracts coming along with stronger antioxidant activity. The efficacy of antioxidant activity was stronger in the hot-water extract of Angelica gigas than other hot-water extracts of medicinal plants. These results suggest that hot-water extraction is an appropriate method to extract materials for growth enhancing and apoptosis protection of SK-N-SH cells, and hot-water extracts of Angelica gigas might be useful materials for protection from aging brain cells.

키워드

참고문헌

  1. Ahn, K. S. 1996. A study on the anticancer and immunostimulating agents from the root of Angelica gigas Nakai. Ph.D Disseration, Korea University, Seoul, Korea.
  2. Blois, M. S. 2003. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200.
  3. Cho, Y. H., B. C. Lee, J. H. Kim, J. H. Kim, H. B. Pyo, Y. H. Zhang, and H. D. Park. 2005. Effect of Artemisia anomala S. Moore on antioxidant activity and melanogenesis. Korean J. Pharmacogn. 36, 273-277.
  4. Choi, S. Y., S. H. Kim, J. S. Kim, T. Y. Ha, S. R. Kim, K. S. Kang, and I. K. Hwang. 2005. Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants. Korean J. Food Sci. Technol. 37, 549-556.
  5. Deliza, R., A. Rosenthal, F.B.D. Abadio, C.H.O. Silva, and C. Castillo. 2005. Application of high pressure technology in the fruit juice processing: Benefits perceived by consumers. J. Food Eng. 67, 241-246. https://doi.org/10.1016/j.jfoodeng.2004.05.068
  6. Folin, O. and W. Denis. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12, 239-249.
  7. Han, S. B., Y. H. Kim, C. W. Lee, S. M. Park, H. Y. Lee, Y. H. Ahn, I. H. Kim, and H. W. Kim. 1998. Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Immunopharmacology 40, 39-48. https://doi.org/10.1016/S0162-3109(98)00026-5
  8. Hwang, J. B. and M. O. Yang. 2002. Comparison of chemical components of Angelica gigas Nakai and Angelica acuriloba kitagawa. Korea J. Food Sci. Technol. 29, 1113-1118.
  9. Islam, M. A., Y. S. Kim, W. J. Jang, S. M. Lee, H. G. Kim, S. Y. Kim, J. O. Kim, and Y. L. Ha. 2008. A mixture of trans, trans conjugated linoleic acid induces apoptosis in MCF-7 human breast cancer cells with reciprocal expression of bax and bcl-2. J. Agric, Food Chem. 56, 5970-5976. https://doi.org/10.1021/jf8004977
  10. Kang, Y. H., Y. K. Park, S. R. Oh, and K. D. Moon. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol. 27, 978-984.
  11. Kim, E. Y. I. H. Baik, J. H. Kim, and S. R. Kim. 2009. Isolation and purification of decursin and decurisinol angelate in Angelica gigas Nakai. J. Korean Soc. Food Sci. Nutr. 38, 653-656. https://doi.org/10.3746/jkfn.2009.38.5.653
  12. Kim, D. H., W. Y. Jung, S. J. Park, J. M. Kim, S. Lee, Y. C. Kim, and J. H. Ryu. 2010. Anti-amnesic effect of ESP-102 on A$\beta$1-42-induced memory impairment in mice. Pharmacol. Biochem. Behav. 97, 239-248. https://doi.org/10.1016/j.pbb.2010.08.005
  13. Kim, M. R., I. H. Kim, and J. H. Shim. 2005. The analysis of volatile components of fresh ginseng, red ginseng and white ginseng by solvent free solid injector (SFSI) techniques. Korean J. Environ. Agric. 24, 164-168. https://doi.org/10.5338/KJEA.2005.24.2.164
  14. Kim, Y. C., H. D. Hong, J. H. Rho, C. W. Cho, Y. K. Rhee, and J. H. Yim. 2007. Changes of phenolic acid contents and radical scavenging activities of ginseng according to steaming times. Korean J. Ginseng Res. 31, 230-236. https://doi.org/10.5142/JGR.2007.31.4.230
  15. Kim, Y. S., R. M. Cerbo, C. K. Hah, K. N. Bahn, J. O. Kim, and Y. L. Ha. 2008. Growth inhibition of osteosarcoma cell MG-63 by a mixture of trans, trans conjugated linoleic aicd isomers: possible mechanistic actions. J. Food Sci. 73, T7-15. https://doi.org/10.1111/j.1750-3841.2008.00747.x
  16. Koo, U., H. J. Lee, D. H. Lee, H. J. Lee, A. R. Ham, E. Y. Cho, and W. Mar. 2008. Anti-oxidative effect of some plant extracts against nitric oxide-induced oxidative stress on neuronal cell. Korean J. Pharmacogn. 39, 290-294.
  17. Korea Institute for Health and Social Affairs, http://www.kihasa.re.kr.
  18. Lee, S. H., Y. S. Lee, S. H. Jung, K. H. Shin, B. K. Kim, and S. S. Kang. 2003. Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch. Pharm. Res. 26, 727-730. https://doi.org/10.1007/BF02976682
  19. Li, L., W. Li, S. W. Jung, Y. W. Lee, and Y. H. Kim. 2011. Protective effects of decursin and decursinol angelate against amyloid $\beta$-protein-induced oxidative stress in the PC12 cell line: The role of Nrf2 and antioxidant enzymes. Biosci. Biotechnol. Biochem. 75, 434-442. https://doi.org/10.1271/bbb.100606
  20. Locatelli, M., F. Travaglia, J. D. Coisson, A. Martelli, C. Stevigny, and M. Arlorio. 2010. Total antioxidant activity of hazelnut skin (Nocciola Piemonte PGI): Impact of different roasting conditions. Food Chem. 119, 1647-1655. https://doi.org/10.1016/j.foodchem.2009.08.048
  21. Maritinez, S., L. Valek, J. Piljac, and M. Metikos-Jukovic. 2005. Determination of wine antioxidant capacity by derivative potentiometric titration with electrogenerated cholorine. Eur. Food Res. Technol. 220, 658-661. https://doi.org/10.1007/s00217-004-1072-5
  22. Moon, J. S., J. S. Park, Y. M. Park, Y. S. Hwang, E. H. Kim, J. W. Park, I. B. Park, S. W. Kim, S. G. Kang, Y. K. Park, and S. T. Jung. 2008. Antioxidant activaties of methanol extracts from prunella uulgaris. J. Korean Soc. Food Sci. Nutr. 37, 1535-1541. https://doi.org/10.3746/jkfn.2008.37.12.1535
  23. Nam, S. H. and M. Y. Kang. 2000. Screening of antioxidative activity of hot-water extracts from medicinal plants. J. Korean Soc. Agric. Chem. Biotechnol. 43, 141-147.
  24. Park, Y. S. 2002. Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. J. Easat Asian Soc. Dietary Life 12, 23-31.
  25. Park, C., H. S. So, S. H. Shin, J. Y. Choi, I. Lee, J. K. Kim, S. Y. Chung, and R. Park. 2007. The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide. Phytother. Res. 21, 81-88. https://doi.org/10.1002/ptr.2028
  26. Salikhova, R. A. and G. G. Poroshenko. 1995. Antimutagenic properties of Angelica archangelica L. Vestn. Ross. Akad. Med. Nauk 1, 58-61.
  27. Santos-Buelga, C., G. Williamson, and M. Saltmarsh. 2003. Methods in polyphenol analysis. pp. 1-16, Royal Society of Chemistry, Cambridge, England.
  28. Seo, H. Y., J. H. Kim, H. P. Song, D. H. Kim, M. W. Byun, J. H. Kwon, and K. S. Kim. 2007. Effects of gamma irradiation on the yields of volatile extracts of Angelica gigas Nakai. Int. J. Radiat. Phys. Chem. 76, 1869-1874. https://doi.org/10.1016/j.radphyschem.2007.03.020
  29. Shin, S., J. H. Jeon, D. Park, J. Y. Jang, S. S. Joo, B. Y. Hwang, S. Y. Choe, and Y. B. Kim. 2009. Anti-inflammatory effects of an ethanol extract of Angelica gigas in a carrageenan-air pouch inflammation model carrageenan-air pouch inflammation model. Exp. Anim. 58, 431-436. https://doi.org/10.1538/expanim.58.431
  30. So, Y. K., Y. L. Ki, H. S. Sang, and C. K. Young. 2005. Four new neuroprotective dihydropyranocoumarins from Angelica gigas. J. Nat. Prod. 68, 56-59. https://doi.org/10.1021/np049705v
  31. Sohn, E. S., S. W. Kim, Kang, and S. P. Lee. 2004. Domestic R&D trend analysis of functional food using medical plants. Applied Chem. 8, 470-473.
  32. Turkmen, N., F. Sari, and Y. Sedat Velioglu. 2006. Effect of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem. 99, 835-841. https://doi.org/10.1016/j.foodchem.2005.08.034
  33. Yu, H. H., S. J. Seo, Y. H. Kim, H. Y.Lee, R. K. Park, H. S. So, S. l. Jang, and Y. O. You. 2006. Protective effect of Rehmannia glutinosa on the cisplatin-induced damage of HEI-OCI auditory cells through scavenging free radicals. J. Ethnopharmacol. 107, 383-388. https://doi.org/10.1016/j.jep.2006.03.024
  34. Rakib, Md. A., Y. S. Kim, W. J. Jang, B. D. Choi, J. O. Kim, I. K. Kong, and Y. L. Ha. 2010. Attenuation of 12-O-tetradecanoylphorbol- 13-acetate (TPA)-induced gap junctional intercellular communication (GJIC) inhibition in MCF-10A cells by c9,t11-conjugated linoleic acid. J. Agric. Food Chem. 58, 12022-12030. https://doi.org/10.1021/jf103205c

피인용 문헌

  1. Effects of different parts of Angelica gigas Nakai on brain damages and neuronal death in transient middle artery occlusion/reperfusion-induced ischemic rats vol.29, pp.6, 2014, https://doi.org/10.6116/kjh.2014.29.6.85.
  2. Neuroprotective effect of the water extract of Angelicae Gigantis Radix Palva in ischemic stroke rats vol.27, pp.2, 2012, https://doi.org/10.6116/kjh.2012.27.2.61
  3. Lack of Cytotoxicity of the Colorant in Conjugated Linoleic Acid against Human Cancer and Normal Cells vol.22, pp.8, 2012, https://doi.org/10.5352/JLS.2012.22.8.1099