Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Extraction Methods of Medicinal Plants on Human Growth of Neuroblastoma SK-N-SH Cells

추출방법에 따른 한약재의 인체신경모세포 SK-N-SH 보호 효과

  • Kwon, Jung-Min (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University) ;
  • Moon, Yeon-Gyu (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University) ;
  • Kim, Young-Suk (Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Jung, Ji-Young (Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Ha, Yeong-Lae (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University) ;
  • Yang, Jae-Kyung (Institute of Agriculture & Life Sciences, Gyeongsang National University)
  • 권정민 (경상대학교 응용생명과학부) ;
  • 문연규 (경상대학교 응용생명과학부) ;
  • 김영숙 (경상대학교 농업생명과학연구원) ;
  • 정지영 (경상대학교 농업생명과학연구원) ;
  • 하영래 (경상대학교 응용생명과학부) ;
  • 양재경 (경상대학교 농업생명과학연구원)
  • Received : 2011.07.07
  • Accepted : 2011.08.26
  • Published : 2011.08.30
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Abstract

Extraction methods of medicinal plants were evaluated for growth enhancing effects of human neuroblastoma SK-N-SH cells. Hot-water extraction (reflux for 5 hr), hot-water extraction post treatment (100$^{\circ}C$ or 120$^{\circ}C$, 90 min) and ethanol extraction (reflux for 5 hr) methods were applied to Angelica gigas, Rhemania glutinosa, Paeonia lactiflora and Cnidium officinale samples to extract their constituents. Cells were treated for 2 hr with various concentrations of extracts (0, 0.5, 1, 2, 4 ${\mu}g/{\mu}l}$ media) prior to $H_2O_2$ (250 ${\mu}M$) treatment for 2 hr to provide oxidative stress. Cell viability, caspase-3 expression and apoptosis were measured for cells treated with sample extracts. Hot-water extract exhibited a stronger growth enhancing and apoptosis protecting ability than other extracts. These activities were shown at less than 1 ${\mu}g/{\mu}l}$ concentration, and not greater than 2 ${\mu}g/{\mu}l}$ concentration. Hot-water extract contained more polyphenolic compounds than other extracts coming along with stronger antioxidant activity. The efficacy of antioxidant activity was stronger in the hot-water extract of Angelica gigas than other hot-water extracts of medicinal plants. These results suggest that hot-water extraction is an appropriate method to extract materials for growth enhancing and apoptosis protection of SK-N-SH cells, and hot-water extracts of Angelica gigas might be useful materials for protection from aging brain cells.

한약재의 추출방법에 따른 인체신경모세포 SK-N-SH에 대한 보호 효과를 연구하였다. 당귀, 건지황, 작약 및 천궁을 시료로 사용하였고, 열수추출(환류냉각, 5 시간), 증숙추출(100$^{\circ}C$ 및 120$^{\circ}C$, 90분 후 열수추출) 방법과 에탄올추출(환류냉각, 5 시간)방법을 비교하였다. 추출물을 농도별로 SK-N-SH 세포에 2 시간 처리한 후 $H_2O_2$로 250${\mu}M$로 2 시간 산화스트레스를 유발한 다음 세포독성 및 apoptosis와 caspase-3의 발현 정도를 측정하였다. 모든 약재의 열수추출물이 다른 추출물보다 세포 증식을 촉진하였고, apoptosis를 억제하였다. 한약재 열수추출물 1${\mu}g/{\mu}l}$ 농도까지는 세포증식을 촉진하였지만, 그 이상의 농도에서는 오히려 감소시켰다. 열수추출물은 다른 추출물보다 총페놀성 화합물이 많이 함유되어 있었고, 항산화능이 높았다. 또한, 이와 같은 효과는 당귀의 열수추출물이 다른 약재 열수추출물보다 우수하였다. 본 연구결과는 약재의 열수추출법이 인체신경모세포인 SK-N-SH의 증식과 세포사멸 억제를 위해 가장 우수한 방법이었고, 당귀 열수추출물이 가장 우수한 효과를 지니고 있어 기억력 보호나 상실억제제로 활용할 수 있을 것이다.

Keywords

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