한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제19권4호
  • /
  • Pages.251-256
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  • 2011
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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비타민나무 잎으로부터 항산화활성 관련 Flavonol Glycoside 분리

Isolation of Flavonol Glycoside Related to Antioxidant Activity from Hippophae rhamnoides Leaves

  • 이지원 (삼성생약(주)부설 바이오생명공학연구소) ;
  • 박주희 (삼성생약(주)부설 바이오생명공학연구소) ;
  • 김주성 (제주대학교 생명자원과학대학 생물산업학부) ;
  • 최은영 (삼성생약(주)부설 바이오생명공학연구소) ;
  • 한상노 (삼성생약(주)부설 바이오생명공학연구소) ;
  • 성은수 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 유창연 (강원대학교 한방Bio연구소) ;
  • 권용수 (강원대학교 약학대학 약학과) ;
  • 김명조 (강원대학교 한방Bio연구소)
  • Lee, Ji-Won (Institute of Samsung Herb Medicine Co., Ltd.) ;
  • Park, Ju-Hee (Institute of Samsung Herb Medicine Co., Ltd.) ;
  • Kim, Ju-Sung (College of Applied Life Science, Jeju National University) ;
  • Choi, Eun-Young (Institute of Samsung Herb Medicine Co., Ltd.) ;
  • Han, Sang-No (Institute of Samsung Herb Medicine Co., Ltd.) ;
  • Seong, Eun-Soo (Department of Applied Plant Sciences, Kangwon National University) ;
  • Yu, Chang-Yoen (Oriental Bio-herb Research Institute, Kangwon National University) ;
  • Kwon, Yong-Soo (Department of Pharmacy, Kangwon National University) ;
  • Kim, Myong-Jo (Oriental Bio-herb Research Institute, Kangwon National University)
  • 투고 : 2011.07.27
  • 심사 : 2011.08.16
  • 발행 : 2011.08.30
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초록

The leaves of Hippophae rhamnoides were extracted with methanol and then further fractioned with hexane, ethyl acetate, butanol and water in order to investigate biological activity. Two flavonol glycosides were isolated and identified from ethyl acetate soluble fraction, which showed the strongest antioxidant activity ($RC_{50}$ = 4.33 ${\mu}g/ml$). Isolated two compounds have shown strong free radical scavenging activity. Especially, quercetin 3-O-glucoside ($RC_{50}$ = 2.60 ${\mu}g/ml$) was more active than ${\alpha}$-tocopherol ($RC_{50}$ = 4.67 ${\mu}g/ml$). Total phenol and flavonoid contents were the highest to 4.17 and 1.14 mg/ml in the ethyl acetate soluble fraction. In addition, ethyl acetate soluble fraction was shown to the strongest ${\alpha}$-glucosidase inhibitory activity ($IC_{50}$ = 137.88 ${\mu}g/ml$) among the fractions. The results suggest that leaves of H. rhamnoides could be a potential source of natural antioxidant.

키워드

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