대한수의학회지 (Korean Journal of Veterinary Research)

  • 제51권3호
  • /
  • Pages.239-241
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  • 2011
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Kinetics of HMGB1 level changes in a canine endotoxemia model

  • Yu, Do-Hyeon (Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri) ;
  • Park, Jinho (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chonbuk National University)
  • 투고 : 2011.07.21
  • 심사 : 2011.09.20
  • 발행 : 2011.09.30
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초록

In this study, we investigated the kinetics of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6 and high mobility group box 1 (HMGB1) concentrations in a 48-h model of canine endotoxemia by lipopolysaccharide (LPS) injection. Four healthy beagles were slowly administered 1 mg/kg of LPS diluted in normal saline, while two others were administered normal saline as controls. Blood collection was performed at 0 h (baseline), 1 h and 3 h (for TNF-${\alpha}$), 6 h, 12 h, 24 h and 48 h of the experiment, and cytokine levels were determined using the sandwich ELISA method. Early increments of TNF-${\alpha}$ and IL-6 were observed (< 3 h), but HMGB1 levels increased the most at 12 h of the experiment and gradually decreased until 48 h. During the whole experiment, IL-6 and HMGB1 were sustained over 12 h of LPS injection, whereas TNF-${\alpha}$ decreased within 6 h of LPS injection. Taken together, canine HMGB1 levels increase relatively late (< 12 h) and sustained longer than TNF-${\alpha}$ and IL-6 in response to endotoxin. This is the first study to evaluate canine HMGB1 cytokine from endotoxemia in dogs.

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참고문헌

  1. Bentley AM, Otto CM, Shofer FS. Comparison of dogs with septic peritonitis: 1988-1993 versus 1999-2003. J Vet Emerg Crit Care (San Antonio) 2007, 17, 391-398. https://doi.org/10.1111/j.1476-4431.2007.00251.x
  2. Bone RC. Gram-negative sepsis. Background, clinical features, and intervention. Chest 1991, 100, 802-808. https://doi.org/10.1378/chest.100.3.802
  3. Cai B, Deitch EA, Ulloa L. Novel insights for systemic inflammation in sepsis and hemorrhage. Mediators Inflamm 2010, 2010, 642462.
  4. Hack CE, De Groot ER, Felt-Bersma RJ, Nuijens JH, Strack Van Schijndel RJ, Eerenberg-Belmer AJ, Thijs LG, Aarden LA. Increased plasma levels of interleukin-6 in sepsis. Blood 1989, 74, 1704-1710.
  5. Hauptman JG, Walshaw R, Olivier NB. Evaluation of the sensitivity and specificity of diagnostic criteria for sepsis in dogs. Vet Surg 1997, 26, 393-397. https://doi.org/10.1111/j.1532-950X.1997.tb01699.x
  6. Hosgood G, Salisbury SK. Generalized peritonitis in dogs: 50 cases (1975-1986). J Am Vet Med Assoc 1988, 193, 1448-1450.
  7. Kenney EM, Rozanski EA, Rush JE, Delaforcade- Buress AM, Berg JR, Silverstein DC, Montealegre CD, Jutkowitz LA, Adamantos S, Ovbey DH, Boysen SR, Shaw SP. Association between outcome and organ system dysfunction in dogs with sepsis: 114 cases (2003-2007). J Am Vet Med Assoc 2010, 236, 83-87. https://doi.org/10.2460/javma.236.1.83
  8. Murua Escobar H, Meyer B, Richter A, Becker K, Flohr AM, Bullerdiek J, Nolte I. Molecular characterization of the canine HMGB1. Cytogenet Genome Res 2003, 101, 33-38. https://doi.org/10.1159/000073415
  9. Remick DG, Bolgos GR, Siddiqui J, Shin J, Nemzek JA. Six at six: interleukin-6 measured 6 h after the initiation of sepsis predicts mortality over 3 days. Shock 2002, 17, 463-467. https://doi.org/10.1097/00024382-200206000-00004
  10. Waage A, Brandtzaeg P, Halstensen A, Kierulf P, Espevik T. The complex pattern of cytokines in serum from patients with meningococcal septic shock. Association between interleukin 6, interleukin 1, and fatal outcome. J Exp Med 1989, 169, 333-338. https://doi.org/10.1084/jem.169.1.333
  11. Wang H, Bloom O, Zhang M, Vishnubhakat JM, Ombrellino M, Che J, Frazier A, Yang H, Ivanova S, Borovikova L, Manogue KR, Faist E, Abraham E, Andersson J, Andersson U, Molina PE, Abumrad NN, Sama A, Tracey KJ. HMG-1 as a late mediator of endotoxin lethality in mice. Science 1999, 285, 248-251. https://doi.org/10.1126/science.285.5425.248
  12. Winkler KP, Greenfield CL. Potential Prognostic Indicators in Diffuse Peritonitis Treated with Open Peritoneal Drainage in the Canine Patient. J Vet Emerg Crit Care (San Antonio) 2000, 10, 259-265. https://doi.org/10.1111/j.1476-4431.2000.tb00011.x
  13. Yilmaz Z, Ilcol YO, Ulus IH. Endotoxin increases plasma leptin and ghrelin levels in dogs. Crit Care Med 2008, 36, 828-833. https://doi.org/10.1097/01.CCM.0B013E3181611F5AA
  14. Yu DH, Nho DH, Song RH, Kim SH, Lee MJ, Nemzek JA, Park J. High-mobility group box 1 as a surrogate prognostic marker in dogs with systemic inflammatory response syndrome. J Vet Emerg Crit Care (San Antonio) 2010, 20, 298-302. https://doi.org/10.1111/j.1476-4431.2010.00539.x