Temporal Variations of Skin Mucus Cells of Misgurnus mizolepis (Cobitidae) by a Change of Water Temperature

수온변화에 의한 미꾸라지, Misgurnus mizolepis 표피점액세포의 일시적 변화

  • Oh, Min-Ki (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Park, Jong-Young (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University)
  • 오민기 (전북대학교 자연과학대학 생명과학과.생물다양성연구소) ;
  • 박종영 (전북대학교 자연과학대학 생명과학과.생물다양성연구소)
  • Received : 2011.05.20
  • Accepted : 2011.06.19
  • Published : 2011.06.30

Abstract

We studied any variations of skin mucus cells of Misgurnus mizolepis caused by inducing a great change of water temperature including high temperature- and low temperature-adapted groups and then compared them with a control group of normal water condition. The high temperature-adapted group showed no significant different in size and number of the mucus cell (P>0.01), whereas in the low temperature-adapted group, the surface area of mucus cell layer and shape of its mucus cell, and the number of mucus cell remarkably increased in all the skin regions of dorsum, lateral region and occiput (P<0.01). Returned to the same condition as the control group, the low temperature-adapted groups showed the same features that appear in the normal water condition (P<0.01). Based on these results, the skin mucus cells of M. mizolepis seems to be very sensitive to cold water temperature and therefore they may play a key role in assessment of its environmental conditions.

인위적 수온변화에 의한 미꾸라지 피부의 점액세포 반응을 확인하기 위해 고온($32^{\circ}C$)과 저온($4^{\circ}C$)에 적응시킨 실험군의 표피조직을 실온($18^{\circ}C$)의 대조군 집단과 비교 분석하였다. 그 결과, 고온적응 설험군에서는 대조군과 유의한 차이를 보이지 않았으나, 저온적응 실험군에서는 등, 체측, 후두부의 점액세포 수와 크기가 현저하게 증가하는 경향을 보였다(P<0.01). 또한 저온적응 실험군의 수온을 증가시켜 상온에서 재 적응시킨 결과에서도 피부 정액세포의 수와 크기가 현저히 감소하는 경향을 보였다(P<0.01). 따라서 미꾸라지 피부의 점액세포가 저온에 매우 민감하게 반응하는 양상은 어류의 조직학적 환경지표로서 유용할 것으로 여겨진다.

Keywords

References

  1. Fishelson, L. 1996. Skin morphology and cytology in marine eels adapted to different lifestyles. The Anatomical Record, 246: 15-29. https://doi.org/10.1002/(SICI)1097-0185(199609)246:1<15::AID-AR3>3.0.CO;2-E
  2. Kim, I.S., Y. Choi, C.R. Lee, Y.J. Lee, P.J. Kim and J.H. Kim. 2005. Illustrated book of Korean fishes. Kyo-Hak Publishing Co., Ltd., Seoul, 615pp. (in Korean)
  3. Liem, K.F. 1967. Functional morphology of the integumentary, respiratory, and digestive systems of the synbranchoid fish Monopterus albus. Copeia, 1967: 375-388. https://doi.org/10.2307/1442128
  4. Mittal, A.K. and J.S.D. Munshi. 1971. A comparative study of the structure of the skin of certain air-breathing fresh-water teleosts. J. Zool., 163: 515-532.
  5. Oh, M.K. and J.Y. Park. 2008. Seasonal change of the skin morphology of muddy loach, Misgurnus anguillicaudatus (Cobitidae) from Korea. Korean J. Ichthyol., 20: 90-96.
  6. Oh, M.K. and J.Y. Park. 2009. Seasonal variation of skin structure in a ricefield-dwelling mud loach Misgurnus mizolepis (Cobitidae) from Korea. Korean J. Ichthyol., 21: 87-92.
  7. Oh, M.K. and J.Y. Park. 2010. Seasonal change of skin mucus cells of Misgurnus mizolepis (Cobitidae) dwelling in a natural stream in Korea. Korean J. Ichthyol., 22: 230-237.
  8. Oh, M.K. and J.Y. Park. 2011. Change of skin mucus cells related to aerial exposure of Misgurnus mizolepis (Cobitidae) dwelling in a rice field. Korean J. Ichthyol., 23: 70-74.
  9. Park, J.Y. and I.S. Kim. 1999. Structure and histochemistry of skin of mud loach, Misgurnus anguillicaudatus (Pisces, Cobitidae), from Korea. Korean J. Ichthyol., 11: 109-116.
  10. Park, J.Y., I.S. Kim and S.Y. Kim. 2001. Morphology and histochemistry of the skin of the mud loach, Misgurns mizolepis, in relation to cutaneous respiration. Korean J. Biol. Sci., 5: 303-308. https://doi.org/10.1080/12265071.2001.9647619
  11. Presnell, J.K. and M.P. Schreibman. 1997. Humanson's animal tissue techniques. The Johns Hopkins Univ. Press, London, 572pp.
  12. Sadovy, Y., J.E. Randall and M.B. Rasotto. 2005. Skin structure in six dragonet species (Gobiesociformes; Callionymidae): interspecific differences in glandular cell types and mucus secretion. J. Fish Biol., 66: 1411-1418. https://doi.org/10.1111/j.0022-1112.2005.00692.x
  13. Sayer, M.D.J. 2005. Adaptations of amphibious fish for surviving life out of water. Fish and Fisheries, 6: 186-211. https://doi.org/10.1111/j.1467-2979.2005.00193.x
  14. Shephard, K.L. 1994. Functions for fish mucus. Reviews in Fish Biology and Fisheries, 4: 401-429. https://doi.org/10.1007/BF00042888
  15. So, S.Y., J.W. Hur and J.Y. Lee. 2008. Variation of oxygen consumption, operculum movement number and jemoglovin by water temperature change in rainbow trout Oncorhynchus mykiss. Korean J. Ichthyol., 20: 239-247. (in Korean)
  16. Tsai, J.C. 1996. Cell renewal in the epidermis of the loach Misgurnus anguillicaudatus (Cypriniformes). J. Zool. Lond., 239: 591-599. https://doi.org/10.1111/j.1469-7998.1996.tb05945.x