Beneficial Effect of a Collagen Peptide Supplement on the Epidermal Skin Barrier

콜라겐 펩타이드의 피부 장벽 보호 효과

  • 김정기 ((주)아모레퍼시픽 기술연구원) ;
  • 이지해 ((주)아모레퍼시픽 기술연구원) ;
  • 배일홍 ((주)아모레퍼시픽 기술연구원) ;
  • 서대방 ((주)아모레퍼시픽 기술연구원) ;
  • 이상준 ((주)아모레퍼시픽 기술연구원)
  • Received : 2010.12.31
  • Accepted : 2011.05.18
  • Published : 2011.08.31


Recent studies have revealed that collagen peptide (CP) plays a protective role in skin by improving the activity of antioxidants and acts as an inducer of skin regeneration by positive feedback. In this study, we focused on the beneficial effect of reinforcing the CP skin barrier. To evaluate the skin barrier, hairless mice were exposed to UVB irradiation and acetone-treatment, with or without oral administration of CP. The effects on skin appearance, trans-epidermal water loss, epidermal thickness, and cytokine content were measured using bioengineering and histochemical methods. In the CP treated group, the skin had better appearance and less damage than that of the control. Furthermore, in HaCaT cells, the amount of serinepalmytoyl transferase (SPT) mRNA increased by about 1.6-fold after treatment (CP, 100 mg/L), reflecting that CP can induce SPT expression and reinforce the recovery of skin barrier function. These results suggest that CP is not only an anti-wrinkling agent but also a potent candidate as an epidermal moisturizer.

In vivo에서 10주간의 UV 조사에 의해 유발되는 피부 손상 및 2주간의 아세톤 도포에 의해 유발되는 급성 피부장벽손상에 대한 콜라겐 펩타이드의 보호 효능을 관찰한 결과, 주름 증가, 비정상적 각질 세포 증식에 의한 피부 두께 증가, 염증성 사이토카인의 증가 등이 콜라겐 펩타이드의 경구 섭취에 의해 개선됨을 확인하여, 콜라겐 펩타이드가 피부 손상을 방어하고, 피부 장벽회복기능이 정상적으로 작용할 수 있도록 도움을 주는 것을 알 수 있었다. 콜라겐 펩타이드의 피부 장벽 회복 기전을 살펴보기 위하여 사람 각질세포를 이용하여 평가한 결과, 콜라겐 펩타이드는 SPT 발현을 농도 의존적으로 증가시킴을 확인하였으며, 이를 통하여 콜라겐 펩타이드가 광노화 및 급성 피부장벽 손상에 의해 유발되는 피부 진피 및 표피 층의 손상을 회복 혹은 보호하는 효능이 있음을 알 수 있었다. 이상의 결과로부터 콜라겐 펩타이드가 광노화 보호 또는 피부장벽 개선 효능을 갖는 새로운 미용 식품 소재로써 이용 가능성이 높음을 확인할 수 있다.



  1. Park CS. Epidermal homeostasis and dry skin management. J. Soc. Cosmet. Scientists Korea. 34:1-8 (2008)
  2. Choi EH, Lee IW, Ahn SK, Lee SJ. The epidermal change of hairless mouse skin by repeated skin barrier disruption. Kor. J. Investig. Dermatol. 4: 136-142 (1997)
  3. Choi EH, Kim MJ, Ahn SK, Park WS, Son ED, Nam GW, Chang I, Lee SH. The skin barrier state of aged hairless mice in a dry environment. Brit. J. Dermatol. 147: 244-249 (2002)
  4. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Skin barrier disruption by acetone: observation in a hairless mouse skin model. Arch. Dermatol. Res. 301: 609-613 (2009)
  5. Ashida Y, Ogo M, Denda M. Epidermal interleukin-1a generation is amplified at low humidity: implications for the pathogenesis of inflammatory dermatoses. Brit. J. Dermatol. 144: 238-243 (2001)
  6. Park CS. Skin barrier and beauty foods. Food Sci. Ind. 40: 19-26 (2008)
  7. Lee SJ. Novel natural products as active material for beauty food. Food Sci. Ind. 40: 10-18 (2008)
  8. Zague V. A new view concerning the effects of collagen hydrolysate intake on skin properties. Arch. Dermatol. Res. 300: 479-483 (2008)
  9. Lee JH, Seo JH, Park YH, Kim WG, Lim KM, Lee SJ. The effect of hydroxyproline and Pro-Hyp dipeptide on UV-damaged skin of hairless mice. Korean J. Food Sci. Technol. 40: 436-442 (2008)
  10. Kim JK, Lee JH, Yang MS, Seo DB, Lee SJ. Beneficial effect of collagen peptide supplement on anti-aging against photodamage. Korean J. Food Sci. Technol. 41: 441-445 (2009)
  11. Shigemura Y, Iwai K, Morimatsu F, Iwamoto T, Mori T, Oda C, Taira T, Park EY, Nakamura Y, Sato K. Effect of prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin. J. Agr. Food Chem. 57: 444-449 (2009)
  12. Kikuta T, Tsuda Y, Kojima H, Sasaki Y. The development of highly functional collagen tripeptide. Fragr. J. 11: 61-67 (2003)
  13. Matsumoto H, Ohara H, Itoh K, Nakamura Y, Takahashi S. Clinical effects of fish type I collagen hydrolysate on skin properties. ITE Lett. 7: 386-390 (2006)
  14. Haratake A, Uchida Y, Schmuth M. UVB-induced alterations in permeability barrier function: roles for epidermal hyperproliferation and thymocyte-mediated response. J. Invest. Dermatol. 108: 769-775 (1998)
  15. Jiang SJ, Chu AW, Lu ZF, Pan MH, Che DF, Zhou XJ. Ultraviolet B-induced alterations of the skin barrier and epidermal calcium gradient. Exp. Dermatol. 16: 985-992 (2007)
  16. Davis BH, Chen A, Beno DW. Raf and mitogen-activated protein kinase regulate stellate cell collagen gene expression. J. Biol. Chem. 271: 11039-11042 (1996)
  17. Houben E, De Paepe K, Rogiers V. A keratinocyte's course of life. Skin Pharmacol. Physiol. 20: 122-132 (2007)
  18. Cho HS, Lee MS, Lee JW, No KO, Park SK, Lee HS, Kang SJ, Cho WG, Park HJ, Oh KW, Hong JT. Anti-wrinkling effects of the mixture of vitamin C, vitamin E, pycnogenol and evening primrose oil, and molecular mechanisms on hairless mouse skin caused by chronic ultraviolet B irradiation. Photodermatol. Photoimmunol. Photomed. 23: 155-162 (2007)
  19. Lee MJ, Won CH, Lee SR, Kim JS, Oh IG, Hwang EI, Kim NM, Kang BC, Chung JH. Oral administration of KTNG0345 prepared from red ginseng extracts reduces UVB-induced skin wrinkle formation in hairless mice. J. Ginseng Res. 32: 48-56 (2008)
  20. Jeon HY, Kim JK, Kim WG, Lee SJ. Curcumin, vitamin C and vitamin E mixture prolonged the antioxidant effect beyond that of each alone and offer synergistic antioxidant effect in vivo. Food Sci. Biotechnol. 17: 1151-1155 (2008)
  21. Ohara H, Matsumoto H, Ito K, Iwai K, Sato K. Comparison of quantity and structures of hydroxyproline- containing peptides in human blood after oral ingestion of gelatin hydrolysates from different sources, J. Agr. Food Chem. 55: 1532-1535 (2007)

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