한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제34권2호
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  • Pages.111-116
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  • 2011
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
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국내 인공수정센터의 웅돈에 대한 번식 관련 바이러스 조사

A survey of viruses associated with reproductive failure in boar semen in Korean artificial insemination centers

  • 김영훈 (강원대학교 수의학과대학 및 동물의학종합연구소) ;
  • 천봉수 (강원대학교 수의학과대학 및 동물의학종합연구소) ;
  • 김성재 (강원대학교 수의학과대학 및 동물의학종합연구소) ;
  • 한정희 (강원대학교 수의학과대학 및 동물의학종합연구소)
  • Kim, Yeong-Hun (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Chun, Bong-Su (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Sung-Jae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Han, Jeong-Hee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2011.03.14
  • 심사 : 2011.03.25
  • 발행 : 2011.06.30
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초록

Artificial insemination (AI) of swine is a very useful reproductive tool and that offers convenience in the Korean swine industry. Since many viruses have been reported to be excreted through boar semen, we investigated the presence of antibodies and antigens against viruses causing reproductive failure in semen of boar in 349 semen samples collected from six Korean AI centers. Viral antigens were detected by polymerase chain reaction (PCR) or reverse transcription-PCR predominantly. The results was as follows. The major reproductive failure causing factor was porcine circovirus type 2 (PCV2), followed by porcine reproductive and respiratory syndrome virus (PRRSV) ($X^2$=166.64, P<0.001). PCV2 and PRRSV, Japanese encephalitis virus (JEV), encephalomyocarditis virus (EMCV) was detected in 73 samples (20.9%), 44 samples (12.6%), 4 samples (1.1%), 3 samples (0.9%), respectively and porcine parvovirus in one sample (0.3%) Classical swine fever virus (CSFV), bovine viral diarrhea virus and Aujeszky's disease virus (ADV) were not detected. Enzyme-linked immunosorbent assay was carried out in 111 serum samples from three AI centers. In most pigs, antibodies response was showed prominently in CSFV (105 sera, 94.6%) ($X^2$=82.580, P<0.001), followed by, in PRRSV (100 sera, 90.1%), PCV2 (92 sera, 90.1%), and PPV (8 sera, 82.9%). ADV antibody was not detected. Thus, the experimental results will be used for the base data, with respect to the state of viral stillbirth in general pig farms, as well as AI centers and breeding farms in Korea.

키워드

참고문헌

  1. 김병한, 권창희, 안수환, 이재진. 1992. 돼지 바이러스 질병 감염에 의한 유사산 실태조사. 대한수의학회지 32(3): 365-368.
  2. 류영수, 박최규, 이창희. 1998. Nested PCR 및 RT-PCR을 이용한 PRRSV의 정액내 신속 감별진단법. 대한수의학회지 38(1): 77-83.
  3. 박최규, 윤하정, 이창희, 정병열, 이경기, 김현수. 2008. 혈청학적 분석을 통한 돼지 생식기호흡기증후군의 농장단위 감염유형. 대한수의학회지 48(1): 67-73.
  4. 박최규, 이은섭, 윤하정, 위성환, 송재영, 문운경, 최은진, 김현수, 이주호, 안수환. 2006. 2003년 한국의 돼지콜레라 전국적 확산에 대한 기술역학. 대한수의학회지 46(3): 197-206
  5. 이경기, 현방훈, 김재조, 김인중, 김병국, 박최규, 소병재, 이오수. 2001. PCR기법 이용 돼지바이러스성 유사산 질병원인체 정밀진단. 국립수의과학검역원 연구보고서: 62-66.
  6. 추금숙, 한규삼, 한재철, 송희종. 2004. RT-PCR과 ELISA를 이용한 PRRS 진단 및 항체가 조사. 한국가축위생학회지 27(3): 273-280.
  7. Choi C, Chae C. 2003. Detection of classical swine fever virus in boar semen by reverse transcription-polymerase chain reaction. J Vet Diagn Invest 15(1): 35-41. https://doi.org/10.1177/104063870301500108
  8. Cottrell TS, Friendship RM, Dewey CE, Allan G, Walker I, McNeilly F. 1999. A study investigating epidemiological risk factors for porcine circovirus type II in Ontario. Pig J 44: 10-17.
  9. Francki RIB, Fauquet CM, Knudson DL, Brown F. 1991. Fifth report of the international committee on taxonomy of viruses. Springer Verlag, Wien New York: 223-233.
  10. Gerrits RJ, Lunney JK, Johnson LA, Pursel VG, Kraeling RR, Rohrer GA, Dobrinsky JR. 2005. Perspectives for artificial insemination and genomics to improve global swine populations. Theriogenology 63(2): 283-299. https://doi.org/10.1016/j.theriogenology.2004.09.013
  11. Guerin B, Pozzi N. 2005. Viruses in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination. Review. Theriogenology 63(2): 556-572. https://doi.org/10.1016/j.theriogenology.2004.09.030
  12. Hennecken M, Stegeman JA, Elbers AR, van Nes A, Smak JA, Verheijden JH. 2000. Transmission of classical swine fever virus by artificial insemination during the 1997-1998 epidemic in The Netherlands: a descriptive epidemiological study. Vet Q 22(4): 228-233. https://doi.org/10.1080/01652176.2000.9695064
  13. Holler LD. 1994. Diagnosis of swine abortion. J Swine Hlth Prod 2(6): 29-31.
  14. Lee CH, Yoon HC, Park CK. 2007. Risk assessment of the potential for a classical swine fever outbreak in Korea based on a herd immunity. Korean J Vet Res 47(4): 429-435.
  15. Madson DM, Patterson AR, Ramamoorthy S, Pal N, Meng XJ, Opriessnig T. 2009. Reproductive failure experimentally induced in sows via artificial insemination with semen spiked with porcine circovirus type 2. Vet Pathol 46(4): 707-716. https://doi.org/10.1354/vp.08-VP-0234-O-FL
  16. Maes D, Nauwynck H, Rijsselaere T, Mateusen B, Vyt P, de Kruif A, Van Soom A. 2008. Diseases in swine transmitted by artificial insemination: an overview. Theriogenology 70(8): 1337-1345. https://doi.org/10.1016/j.theriogenology.2008.06.018
  17. O'Connor B, Gauvreau H, West K, Bogdan J, Ayroud M, Clark EG, Konoby C, Allan G, Ellis JA. 2001. Multiple porcine circovirus 2-associated abortions and reproductive failure in a multisite swine production unit. Can Vet J 42(7): 551-553.
  18. Robertson IB. 1992. Transmission of blue-eared pig disease. Vet Rec 130(21): 478.
  19. Rose N, Larour G, Le Diguerher G, Eveno E, Jolly JP, Blanchard P, Oger A, Le Dimna M, Jestin A, Madec F. 2003. Risk factors for porcine post-weaning multisystemic wasting syndrome (PMWS) in 149 French farrow-to-finish herds. Prev Vet Med 61(3): 209-225. https://doi.org/10.1016/j.prevetmed.2003.07.003
  20. Schmoll F, Lang C, Steinrigl AS, Schulze K, Kauffold J. 2008. Prevalence of PCV2 in Austrian and German boars and semen used for artificial insemination. Theriogenology 69(7): 814-821. https://doi.org/10.1016/j.theriogenology.2007.12.009
  21. Shin J, Torrison J, Choi CS, Gonzalez SM, Crabo BG, Molitor TW. 1997. Monitoring of porcine reproductive and respiratory syndrome virus infection in boars. Vet Microbiol 55(1-4): 337-346. https://doi.org/10.1016/S0378-1135(96)01336-3
  22. Straw BE, D'allairte S, Mengeling WL, Taylor DJ. 2006. Diseases of Swine. 9 eds. Blackwell Publishing, Ames, Iowa: 359-365.
  23. Vannier P. 1999. Infectious causes of abortion in swine. Reprod Dom Anim 34(3-4): 367-376. https://doi.org/10.1111/j.1439-0531.1999.tb01267.x
  24. van Rijn PA, Wellenberg GJ, Hakze-van der Honing R, Jacobs L, Moonen PL, Feitsma H. 2004. Detection of economically important viruses in boar semen by quantitative RealTime PCR technology. J Virol Methods 120(2): 151-160. https://doi.org/10.1016/j.jviromet.2004.04.014
  25. Wasilk A, Callahan JD, Christopher-Hennings J, Gay TA, Fang Y, Dammen M, Reos ME, Torremorell M, Polson D, Mellencamp M, Nelson E, Nelson WM. 2004. Detection of U.S., Lelystad, and European-like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR. J Clin Microbiol 42(10): 4453-4461. https://doi.org/10.1128/JCM.42.10.4453-4461.2004