Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Characterization of Protease Produced by Elizabethkingia meningoseptica CS2-1 and Optimization of Cultural Conditions for Amino Acid Production

닭 우모 분해세균 Elizabethkingia meningoseptica CS2-1이 생산하는 단백질분해효소의 특성 및 아미노산 생산을 위한 배양조건

  • 김세종 (목원대학교 미생물나노소재학과) ;
  • 조천휘 ((주)카프코 생물화학연구소) ;
  • 황경숙 (목원대학교 미생물나노소재학과)
  • Received : 2011.01.26
  • Accepted : 2011.03.22
  • Published : 2011.06.30
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Abstract

A feather-degrading bacterium Elizabethkingia meningoseptica CS2-1 was isolated from compost in a chicken farm. Cultured on a basal medium containing 2% chicken feather, the bacterium showed 729.7 ${\mu}mol/mL$ of amino acid. Optimal culture conditions for feather degradation by E. meningoseptica CS2-1 were $25^{\circ}C$, pH 7.5, and 180 rpm. The optimal pH and temperature for protease activity were 8.0 and $40^{\circ}C$, respectively. The composition of an optimal medium for amino acid production was 0.05% NH4Cl, 0.05% NaCl, 0.03% $K_2HPO_4$, 0.03% $KH_2PO_4$, 0.01% $MgCl_2{\cdot}6H_2O$, 0.1% urea, and 2% chicken feather. Characteristics of amino acids extracted from the optimal medium under the optimal culture conditions of E. meningoseptica CS2-1 were analyzed. The total amino acid content of strain CS2-1 was 1063 ${\mu}mol/mL$, which was 46% higher compared to the basal condition (729.7 ${\mu}mol/mL$). The essential amino acid content in the total amino acid was 315.9 ${\mu}mol/mL$, which was 44% higher than that of the basal condition. Major amino acids were proline (14%), aspartic acid (12%), glutamic acid (11%), serine (10%), alanine (10%), glycine (9%), and tyrosine (7%) by strain CS2-1. These results suggest that strain CS2-1 can be used as a potential microbial resource for the production of amino acid using chicken feathers.

우모분으로부터 높은 함량의 아미노산을 생성하는 Elizabethkingia meningoseptica CS2-1 (729.7 ${\mu}mol/mL$)를 선발하였다. 선발 균주의 protease 활성과 우모 분해능을 위한 최적배지 및 배양조건을 확립하고 우모 분해산물 아미노산의 특성을 조사하였다. E. meningoseptica CS2-1의 최적 배양조건은 $25^{\circ}C$, pH 7.5, 180 rpm이었으며, 효소는 pH 8.0, $40^{\circ}C$에서 가장 높은 활성을 나타내었다. E. meningoseptica CS2-1의 아미노산 생산을 위한 최적 배지조건은 $NH_4Cl$ 0.05%, NaCl 0.05%, $K_2HPO_4$ 0.03%, $KH_2PO_4$ 0.03%, $MgCl_2{\cdot}6H_2O$ 0.01%, urea 0.1%, 닭 우모분 2%이었다. E. meningoseptica CS2-1를 최적 배지 및 배양조건에서 배양한 결과, 아미노산의 총 함량은 1063 ${\mu}mol/mL$로 기본조건 (729.7 ${\mu}mol/mL$)보다 46%로 향상되었고 필수 아미노산 함유량은 315.9 ${\mu}mol/mL$로 기본조건 (219.3 ${\mu}mol/mL$)보다 44%로 향상되었다. 닭 우모분으로부터 추출되 는 17 종류의 아미노산 중 proline (14%), aspartic acid (12%), serine (10%), alanine (10%), glycine (9%), tyrosine (7%)이 주요 아미노산으로 추출되는 특징을 나타내었다. 따라서, E. meningoseptica CS2-1은 닭 우모로부터 아미노산 생산을 위한 미생물유전자원 소재로서 잠재적 가치가 클 것으로 판단된다.

Keywords

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