Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Phosphoproteomic Analysis of the Brain of Ovariectomized Adult Rat

  • Santos, Ilyn Lyzette (Department of Animal Science and Molecular Genetics, Kyungpook National University) ;
  • Kim, Kil-Soo (Department of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Jong-Sang (Department of Animal Science and Molecular Genetics, Kyungpook National University) ;
  • Lim, Jin-Kyu (Department of Animal Science and Molecular Genetics, Kyungpook National University)
  • Received : 2011.04.30
  • Accepted : 2011.05.24
  • Published : 2011.06.30
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Abstract

Aging in females is associated with a reduced metabolic function, increased incidence of neurodegenerative diseases, and cognitive dysfunction, as a result of loss in gonadal function. The change can alter the states of phosphorylation on the proteins, which cause dramatic changes in the cellular location or activity of the proteins. In this study, the differential phosphorylation of the proteins responsible for the functions related to cognition was studied using the ovariectomized adult rats. Phosphoproteomic analysis using the cerebral and hippocampal tissues could identify 51 differentially phosphorylated proteins including 12 proteins for energy metabolism, 8 cytoskeletal proteins, 6 signaling proteins, and other functional proteins in the ovariectomized rats. Further, anti-oxidative enzymes, superoxide dismutase and peroxiredoxin-2, which are known to be inactivated by phosphorylation, were found to be differentially phosphorylated in the cerebellum and hippocampus of the ovariectomized rats, respectively. Many of the deactivated proteins by differential phosphorylation identified in this study were overlapped to those of Alzheimer's disease cases. These results will provide information for neurodegenerative learning and memory impairments in women as brought about by menopause.

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References

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