Parasites, Hosts and Diseases

  • 제49권2호
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  • Pages.195-197
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  • 2011
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Molecular Identification of Taenia Tapeworms by Cox1 Gene in Koh Kong, Cambodia

  • Jeon, Hyeong-Kyu (Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine) ;
  • Yong, Tai-Soon (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Sohn, Woon-Mok (Department of Parasitology and Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Hong, Sung-Jong (Department of Medical Environmental Biology, Chung-Ang University College of Medicine) ;
  • Han, Eun-Taek (Department of Parasitology, Gangwon University College of Medicine) ;
  • Jeong, Hoo-Gn (International Cooperation Department, Korea Association of Health Promotion) ;
  • Chhakda, Tep (National Center for Parasitology, Entomology & Malaria Control) ;
  • Sinuon, Muth (National Center for Parasitology, Entomology & Malaria Control) ;
  • Socheat, Duong (National Center for Parasitology, Entomology & Malaria Control) ;
  • Eom, Kee-Seon S. (Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine)
  • 투고 : 2011.03.31
  • 심사 : 2011.04.22
  • 발행 : 2011.06.30
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초록

We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples, Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.

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참고문헌

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피인용 문헌

  1. The Parasitological, Immunological, and Molecular Diagnosis of Human Taeniasis with Special Emphasis on Taenia solium Taeniasis vol.1, pp.4, 2014, https://doi.org/10.1007/s40475-014-0028-5
  2. Epidemiology and genetic diversity of Taenia asiatica : a systematic review vol.7, pp.1, 2014, https://doi.org/10.1186/1756-3305-7-45
  3. Prevalence of Intestinal Helminths among Inhabitants of Cambodia (2006-2011) vol.52, pp.6, 2014, https://doi.org/10.3347/kjp.2014.52.6.661
  4. Comparative analysis of the miRNA profiles from Taenia solium and Taenia asiatica adult vol.8, pp.9, 2014, https://doi.org/10.5897/ajmr12.1218
  5. Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections vol.27, pp.2, 2011, https://doi.org/10.1128/cmr.00122-13
  6. Intestinal Parasite Infections in Symptomatic Children Attending Hospital in Siem Reap, Cambodia vol.10, pp.5, 2015, https://doi.org/10.1371/journal.pone.0123719
  7. Needs of exploring the burden of recent onset seizures due to neurocysticercosis and challenges in southeast Asia focusing on scenario in Malaysia vol.10, pp.4, 2011, https://doi.org/10.1016/j.apjtm.2017.03.024
  8. Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development vol.147, pp.4, 2011, https://doi.org/10.1017/s0031182019001665
  9. Molecular Diagnosis of Taenia saginata Tapeworms from Two Residents of Northern Cambodia vol.58, pp.2, 2020, https://doi.org/10.3347/kjp.2020.58.2.201
  10. Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia vol.13, pp.1, 2020, https://doi.org/10.1186/s13071-020-04095-1