Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Method Development of Verapamil in Presence of NSAIDs using RP-HPLC Technique

  • Sultana, Najma (Dow University of Health Sciences) ;
  • Arayne, M. Saeed (Dow University of Health Sciences) ;
  • Waheed, Abdul (Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi)
  • Received : 2010.12.07
  • Accepted : 2011.05.23
  • Published : 2011.07.20
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Abstract

Verapamil is a calcium channel blocker and is classified as a class IV anti-arrhythmic agent. It is used in the control of supra ventricular tachyarrhythmias, and in the management of classical and variant angina pectoris. It is also used in the treatment of hypertension and used as an important therapeutic agent for angina pectoris, ischemic heart disease, hypertension and hypertrophic cardiomyopathy. Verapamil commonly co-administered with NSAIDs (non-steroidal anti-inflammatory drugs) i.e. diclofenac sodium, flurbiprofen, Ibuprofen, mefanamic acid and meloxicam. A simple and rapid RP-HPLC method for simultaneous determination and quantification of verapamil and NSAIDs was developed and validated. The mobile phase constituted of acetonitrile: water (55:45) whose pH was adjusted at 2.7 and pumped at a flow rate of 2.0 mL $min^{-1}$ at 230 nm. The proposed method is simple, precise, accurate, low cost and least time consuming for the simultaneous determination of verapamil and NSAIDs which can be effectively applied for the analysis of human serum.

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