Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Synthesis and Structural Studies of an Organic Complex and its Association with BSA

  • Meng, Fa-Yan (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Yu, Sheng-Rong (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Liang, Li-Xi (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Zhong, Xue-Ping (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Wang, Li (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Zhu, Jin-Mei (College of Chemistry and Chemical Engineering, Guangxi University) ;
  • Lin, Cui-Wu (College of Chemistry and Chemical Engineering, Guangxi University)
  • Received : 2011.05.06
  • Accepted : 2011.05.19
  • Published : 2011.07.20
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Abstract

The self-assembly of one novel organic complex based on chlorogenic acid (HCA) and 2,2'-bipyridine (2,2'-bipy) has been synthesized and characterized. The complex achieved by hydrogen-bonding interactions, adopted a 1:1 stoichiometry in a solid state. The proton transfer occurred from the carboxyl oxygen to the aromatic nitrogen atom to form salts CA${\cdot}$(2,2'-Hbipy), the 2,2'-Hbipy molecule individually occupies the pseudo-tetragonum that is formed with CA. In this paper, the interactions of CA${\cdot}$(2,2'-Hbipy) with bovine serum albumin (BSA) were studied by fluorescence spectrometry. For CA${\cdot}$(2,2'-Hbipy), HCA and 2,2'-bipy, the average quenching constants for BSA were $2.4384{\times}10^4$, $4.653{\times}10^3$, and $3.059{\times}10^3\;L{\cdot}mol^{-1}$, respectively. The mechanism for protein fluorescence quenching is apparently governed by a static quenching process. The Stern-Volmer quenching constants and corresponding thermodynamic parameters ${\Delta}$H, ${\Delta}$G and ${\Delta}$S were calculated. The binding constants and the number of binding sites were also investigated. The conformational changes of BSA were observed from synchronous fluorescence spectra.

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