Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Extracts from Sarcocarp, Peels, and Seeds of Avocado on Osteoblast Differentiation and Osteoclast Formation

아보카도 과육, 과피 및 씨 추출물이 조골세포 분화 및 파골세포 형성에 미치는 영향

  • Kim, Mi-Jin (Dept. of Food Science and Technology, Keimyung University) ;
  • Im, Nam-Kyung (Dept. of Food Science and Technology, Keimyung University) ;
  • Yu, Mi-Hee (Dept. of Food Science and Technology, Keimyung University) ;
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, In-Seon (Dept. of Food Science and Technology, Keimyung University)
  • 김미진 (계명대학교 식품가공학과) ;
  • 임남경 (계명대학교 식품가공학과) ;
  • 유미희 (계명대학교 식품가공학과) ;
  • 김현정 (계명대학교 전통미생물자원개발 및 산업화 연구센터) ;
  • 이인선 (계명대학교 식품가공학과)
  • Received : 2011.03.14
  • Accepted : 2011.06.27
  • Published : 2011.07.31
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Abstract

Avocado (Persea americana Mill., Family Lauraceae) is an important subtropical crop in the Americas where it has been cultivated for several thousand years. To investigate the bioactivities of avocado, which acts on bone formation, we prepared methanol extracts from the sarcocarp, peels, and seeds of avocado. The methanol extracts of peels and seeds showed higher bone-forming activity than avocado sarcocarp extracts accompanied by MC3T3-E1 osteoblast proliferation and alkaline phosphatase (ALP) activity. Additionally, the extracts of sarcocarp and peel from avocado also decreased tartrate-resistant acid phosphatase (TRAP) activity against differentiation of osteoclasts, derived from mouse bone marrow macrophages. The hexane fraction from avocado peels showed strong bone-forming activity accompanied by osteoblast proliferation and ALP activity (170.7${\pm}$8.4%), and the ethyl acetate fraction from avocado peel decreased TRAP activity (5.2${\pm}$0.3%) and differentiated osteoclasts at 50 ${\mu}g$/mL. Therefore, avocado is expected to be a natural source for developing medicinal agents to prevent bone-related diseases, such as osteoporosis, by increasing osteoblast differentiation and reducing osteoclast activity.

본 연구에서는 아보카도가 골 형성에 미치는 영향을 검토하고자 아보카도 과육, 과피 및 씨로 나누어 각각 메탄올 추출물을 제조하여 osteoblastic MC3T3-E1 cells을 이용한 골 형성능과 마우스 골수 세포로부터 유래된 파골세포를 이용한 골 흡수능을 측정하였다. 아보카도 과육 추출물을 제외한 과피 및 씨 추출물은 조골세포의 증식 및 ALP 활성을 증가시켰으며, 파골세포에 대해서는 아보카도 과육 및 과피추출물에서 세포독성 없이 TRAP 활성을 억제하는 것을 확인하였다. 또한 아보카도 과피의 핵산 분획은 조골세포의 증식 및 ALP 활성을 크게 증가시켰으며, 아보카도 과피 에틸아세테이트 분획은 파골세포의 분화지표인 TRAP 활성을 크게 억제하였다. 따라서 아보카도 과피는 조골세포의 증식과 파골세포의 억제에 관여할 수 있는 우수한 소재로 향후 골다공증의 치료제로서의 개발 가능성을 가진 천연물 소재로 생각된다.

Keywords

References

  1. Cho GJ, Park HT, Shin JH, Kim T, Hur JY, Kim YT, Lee KW, Kim SH. 2009. The relationship between reproductive factors and metabolic syndrome in Korean postmenopausal women: Korea National Health and Nutrition Survey. Menopause 16: 998-1003. https://doi.org/10.1097/gme.0b013e3181a03807
  2. Parfit AM. 1988. Bone remodeling: relationship to the amount and structure of bone the pathogenesis and prevention of fractures. In Osteoporosis, Etiology, Diagnosis and management. Reven Press, New York, NY, USA. p 45-93.
  3. Grigoriadis AE, Wang ZQ, Cecchini MG, Hofstetter W, Felix R, Fleisch HA, Wagner EF. 1994. c-Fos: a key regulator of osteoclast-macrophage lineage determination and bone remodeling. Science 266: 443-448. https://doi.org/10.1126/science.7939685
  4. Hwang KK, Huh NK, Lee JH. 2000. Studies on the signaling molecules in RANK, an osteoclast differentiation receptor. Oral Biol Res 24: 245-255.
  5. Vaananen HK, Zhao H, Mulari M, Halleen JM. 2000. The cell biology of osteoclast function. J Cell Sci 113: 377-381.
  6. Suda TJ, Nakamura I, Jimi E, Takahashi N. 1997. Regulation of osteoclast function. J Bone Miner Res 12: 869-879. https://doi.org/10.1359/jbmr.1997.12.6.869
  7. Del Fattpre A, Teti A, Rucci N. 2008. Osteoclast receptors and signaling. Arch Biochem Biophys 473: 147-160. https://doi.org/10.1016/j.abb.2008.01.011
  8. Oh HJ. 2005. Therapy of osteoporosis in climacteric. J Korea Acad Fam Med 21: 20-27.
  9. Pols HA, Felsenberg D, Hanley DA, Stenpan J, Munoz- Torres M, Wilkin TJ, Qin-sheng G, Galich AM, Vandormael K, Yates AJ, Stych B. 1999. Multinational, placebo-controlled, randomized trial of the effects of alendronate on bone density and fracture risk in postmenopausal women with low bone mass: results of the FOSIT study. Fosamax International Trial Study Group. Osteoporos Int 9: 461-468. https://doi.org/10.1007/PL00004171
  10. Cassidy A. 1996. Physiological effects of phyto-estrogens in relation to cancer and other human health risks. Proc Nutr Soc 55: 399-417. https://doi.org/10.1079/PNS19960038
  11. Boonen S, Broos P, Dequeker J, Bouillon R. 1997. The prevention or treatment of age-related osteoporosis in the elderly by systemic recombinant growth factor therapy (rhIGF-I or rhTGF beta): a perspective. J Intern Med 242: 285-290. https://doi.org/10.1046/j.1365-2796.1997.00133.x
  12. Han KY, Yang D, Chang EJ, Lee Y, Huang H, Sung SH, Lee ZH, Kim YC, Kim HH. 2007. Inhibition of osteoclast differentiation and bone resorption by sauchinone. Biochem Pharmacol 74: 911-923. https://doi.org/10.1016/j.bcp.2007.06.044
  13. Kim JH, Ki JY, Ann JY, Park HJ, Kim HJ, Kwak HB, Oh JM, Kim YK. 2010. Inhibitory effects of Achyranthis bidentatae Radix on osteoclast differentiation and bone resorption. Kor J Herbology 25: 65-74.
  14. Kwak HB, Kim JH, Kim DJ, Kwon YM, Oh JM, Kim YK. 2008. Effect of water extract of deer antler in osteoclast differentiation. Kor J Ori Med Physiol Pathol 22: 891-895.
  15. Park CK, Kim HJ, Kwak HB, Lee TH, Bang MH, Kim CM, Lee Y, Chung DK, Baek NI, Kim J, Lee ZH, Kim HH. 2007. Inhibitory effects of Stewartia koreana on osteoclast differentiation and bone resorption. Int Immunopharmacol 7: 1507-1516. https://doi.org/10.1016/j.intimp.2007.07.016
  16. Tsai HY, Lin HY, Fong YC, Wu JB, Chen YF, Tsuzuki M, Tang CH. 2008. Paeonol inhibits RANKL-induced osteoclastogenesis by inhibiting ERK, p38 and NF-kB pathway. Eur J Pharmacol 588: 124-133. https://doi.org/10.1016/j.ejphar.2008.04.024
  17. Wattel A, Kamel S, Mentaverri R, Lorget F, Prouillet C, Petit JP, Fardelonne P, Brazier M. 2003. Potent inhibitory effect of naturally occurring flavonoids quercetin and kaempferol on in vitro osteoclastic bone resorption. Biochem Pharmacol 65: 35-42. https://doi.org/10.1016/S0006-2952(02)01445-4
  18. Lee YS. 2001. Effect of isoflavones on proliferation and oxidative stress of MC3T3-E1 osteoblast-like cells. Korea Soybean Digest 18: 35-42.
  19. Hierro MT, Tomas MC, Fermandez-martin F, Santa-Maria G. 1992. Determination of the triglyceride composition of avocado oil by high-performance liquid chromatography using a light-scattering detector. J Chromatogr 607: 329-338. https://doi.org/10.1016/0021-9673(92)87092-M
  20. Ding H, Chin YW, Kinghorn AD, D'Ambrosio SM. 2007. Chemopreventive characteristics of avocado fruit. Semin Cancer Biol 17: 386-394. https://doi.org/10.1016/j.semcancer.2007.04.003
  21. Duester KC. 2001. Avocado fruit is a rich source of betasitosterol. J Am Diet Assoc 101: 404-405. https://doi.org/10.1016/S0002-8223(01)00102-X
  22. Kim OK, Murakami A, Nakamura Y, Takeda N, Yoshizumi H, Ohigashi H. 2001. Novel nitric oxide and superoxide generation inhibitors, persenone A and B, from avocado fruit. J Agric Food Chem 48: 1557-1563.
  23. Vinson JA, Su X, Zubik L, Bose P. 2001. Phenol antioxidant quantity and quality in foods: fruits. J Agric Food Chem 49: 5315-5321. https://doi.org/10.1021/jf0009293
  24. Hashimura H, Ueda C, Kawabata J, Kasai T. 2001. Acetyl-CoA carboxylase inhibitors from avocado (Persea americana Mill.) fruits. Biosci Biotechnol Biochem 65: 1656-1658. https://doi.org/10.1271/bbb.65.1656
  25. Pursky D, Kobiler I, Fishman Y, Sims JJ, Midland SL, Keen NT. 1991. Identification of an antifungal compound in unripe avocado fruits and its possible involvement in the quiescent infections of Colletotrichum gloeosporioides. J Phytopathol 132: 319-327. https://doi.org/10.1111/j.1439-0434.1991.tb00127.x
  26. Lee SG, Yu MH, Lee SP, Lee IS. 2008. Antioxidant activities and induction of apoptosis by methanol extracts from avocado. J Korean Soc Food Sci Nutr 37: 269-275. https://doi.org/10.3746/jkfn.2008.37.3.269
  27. Soong YY, Barlow PJ. 2004. Antioxidant activity and phenolic content of selected fruit seeds. Food Chem 88: 411-417. https://doi.org/10.1016/j.foodchem.2004.02.003
  28. Kim JW, Lee HJ, Kang J, Ohk SH, Choi BK, Yoo YJ, Cho KS, Choi SH. 2000. The effect of cyclosporin A on osteoblast in vitro. J Korean Acad Periodontol 30: 747-757.
  29. Green LM, Reade JL, Ware CF. 1984. Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods 70: 257-268. https://doi.org/10.1016/0022-1759(84)90190-X
  30. Hotokezaka H, Sakai E, Kanaoka K, Saito K, Matsuo K, Kitaura H, Yoshida N, Nakayama K. 2002. U0126 and PD98059, specific inhibitors of MEK, accelerate differentiation of RAW264.7 cells into osteoclast-like cells. J Biol Chem 277: 47366-47372. https://doi.org/10.1074/jbc.M208284200
  31. Centrella M, McCarthy TL, Canalis E. 1987. Transforming growth factor beta is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone. J Biol Chem 262: 2869-2874.
  32. Letton RW, Fanti P, Malluche HH. 1990. Regulation of 25-hydroxy vitamin D3 metabolism in cultures of osteoblastic cells. J Bone Miner Res 5: 815-823.
  33. Stein GS, Lian JB, Owen TA. 1990. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEB J 4: 3111-3123.
  34. Bu SY, Hunt TS, Smith BJ. 2009. Dried plum polyphenols attenuate the detrimental effects of TNF-alpha on osteoblast function coincident with up-regulation of Runx2, Osterix and IGF-I. J Nutr Biochem 20: 35-44. https://doi.org/10.1016/j.jnutbio.2007.11.012
  35. Vali B, Rao LG, El-Sohemy A. 2007. Epigallocatechin-3-gallate increases the formation of mineralized bone nodules by human osteoblast-like cells. J Nutr Biochem 18: 341-347. https://doi.org/10.1016/j.jnutbio.2006.06.005
  36. Shin JM, Park CK, Shin E, Jo TH, Hwang IK. 2008. Effects of Scutellaria radix extract on osteoblast differentiation and osteoclast formation. Korean J Food Sci Technol 40: 674-679.
  37. Teitelbaum SL. 2000. Bone resorption by osteoclast. Science 289: 1504-1508. https://doi.org/10.1126/science.289.5484.1504
  38. Mok SK, Shin IIS. 1996. The effects of prostaglandin and dibutyryl cAMP on osteoblastic cell activity and osteoblast generation. J Wonkwang Dental Res Int 6: 43-62.
  39. Karst M, Gorny G, Galvin RJ, Oursler MJ. 2004. Roles of stromal cell RANKL, OPG, and M-CSF expression in biphasic TGF-$\beta$ regulation of osteoclast differentiation. J Cell Physiol 200: 99-106. https://doi.org/10.1002/jcp.20036
  40. Vaananen HK, Horton M. 1995. The osteoclast clear zone is a specialized cell extracellular matrix adhesion structure. J Cell Sci 108: 2729-2732.
  41. Lee JW. 2009. Inhibitory activity of medicinal plants against differentiation of osteoclasts. Kor J Pharmacogn 40: 83-88.
  42. Ji SH, Ahn DH, Jun MR. 2010. Effects of Petasites japonicus and Momordica charantia L. extracts on MC3T3-E1 osteoblast cells. J Korean Soc Food Sci Nutr 39: 203-209. https://doi.org/10.3746/jkfn.2010.39.2.203
  43. Woo WS. 2001. Methods for natural products chemistry. Seoul National University Press, Seoul, Korea. p 1-29.
  44. Auffray C, Sieweke MH, Geissmann F. 2009. Blood monocytes: development, heterogeneity, and relationship with dendritic cells. Annual Rev Immunol 27: 669-692. https://doi.org/10.1146/annurev.immunol.021908.132557

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