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Chlrorophylls and their Degradation Products using High Performance Liquid Chromatography (HPLC), with Data from Suspended and Sinking Particulate Matter in Prydz Bay, Antarctica

  • Noh, Il (Division of Marine Environment and Bioscience, Korea Maritime University)
  • 투고 : 2011.05.18
  • 심사 : 2011.06.22
  • 발행 : 2011.06.30

초록

Suspended and sinking particles were collected in austral summer during ODP Leg 119 to the Indian Ocean sector of the Antarctic Ocean. Field work was carried out at four sampling sites in Prydz Bay. Two of these sites were located in the Outer Bay, and two in the Inner Bay. At the four locations, a total of ten deployments of a sediment trap array were made. The concentrations of chlorophylls and their degradation products both in suspended and sinking particulate matter in Prydz Bay were analyzed using HPLC. Chlorophylls a and c were the dominant algal pigments both in suspended and sinking particles. Because of the abundance of fecal pellets at Site 740, the mean fluxes at 200 m averaged 6 fold greater than that at 50 m. This implies that a dense swarm of zooplankters, presumably large copepods and/or salps, may "feed and excrete" mainly in between 100-200 m depths at this site, closest to land in Prydz Bay. Interestingly, The flux of phaeophorbide a was generally similar in magnitude to that of chlorophyll a throughout the study areas. This is an evidence that materials escaping from near-surface regions in austral summer derive mainly from the gazing of zooplankters. "New production" from sediment-trapped CHL pigment fluxes in Prydz Bay was estimated using f-ratio of 0.15, ranging from 520 to $1,605\;{\mu}gC\;m^{-2}\;day^{-1}$.

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참고문헌

  1. Abaychi, J.K. and Riley, J.P.(1979), "The determination of phytoplankton pigments by high-performance liquid chromatography", Anal. Chim. Acta, Vol 107, pp. 1-11. https://doi.org/10.1016/S0003-2670(01)93190-3
  2. Allanson, B.R., Hart, R.C., and Lutjeharms, J.R.E.(1981), "Observations on the nutrients, chlorophyll and primary production of the Southern Ocean south of Africa", South African J. Antarctic Res., Vol. 10/11, pp. 3-14.
  3. Alldredge, A.L.(1979), "The chemical composition of microscopic aggregates in two neritic seas", Limnol. Oceanogr., Vol. 254, pp. 855-866.
  4. American Public Health Association.(1989), "High-performance liquid chromatographic determination of algal chlorophylls and their degradation products", pp. 1039. In: Standard Methods for the Examination of Water and Wastewater, 17th Edition, Washington, D.C.
  5. Barrett, J. and Jeffrey, S.W.(1971), "A note on the occurrence of chlorophyllase in marine algae", J. Exp. Mar. Biol. Ecol., Vol. 7, pp. 255-262. https://doi.org/10.1016/0022-0981(71)90008-6
  6. Bidigare, R.R.(1989), "Photosynthetic pigment composition of the brown tide alga: Unique chlorophyll and carotenoid derivatives", pp. 57-75, In: Novel Phytoplankton Blooms (E Cosper, E. J. Carpenter and M. Bricelj, eds.), Coastal and Estuarine Studies, Vol. 35. Springer-Verlag, Berlin.
  7. Bidigare, R.R., Kennicutt, M.C., and Brooks, J.M.(1985), "Rapid determination of chlorophylls and degradationproducts by high performance liquid chromatography", Limnol. Oceanogr., Vol. 30, pp. 432-435. https://doi.org/10.4319/lo.1985.30.2.0432
  8. Bidigare, R.R., Frank, T.J., Zastrow C., and Brooks, J.M.(1986), "The distribution of algal chlorophylls and their degradation products in the Southern Ocean", Deep-Sea Res., Vol. 33, pp. 923-937. https://doi.org/10.1016/0198-0149(86)90007-5
  9. Biggs, D.C., Berkowitz, S.P., Altabet, M.A., Bidigare, R.R, DeMaster, D.J, Dunbar, R.B., Leventer, A.N., Macko, S.A., Nittroluer, C.A., and Ondrusek, M.E.(1988), "Upper-ocean particulate fluxes in the Weddell Sea", Proc. ODP, Init. Repts., 113: pp. 77-86, College Stadon, TX (Ocean Drilling Program).
  10. Biggs, D.C., Berkowitz, S.P., Altabet, M.A., Bidigare, R.R., DeMaster, D.J., Macko, S.A., Ondrusek, M.E., and Noh, I.(1989), "Cooperative study of upper ocean particulate fluxes", Proc. ODP, Init. Repts., 119: pp. 109-120.
  11. Bogdanov, D.V., Sokolov, V.A., and Khromov, N.S.(1969), "Regions of high biological and commercial productivity in Gulf of Mexico and Caribbean Sea", Oceanology, Vol. 8, pp. 371-381.
  12. Brewer, P.G., Nozaki, Y., Spencer, D.W., and Fleer, A.P.(1980), "Sediment trap experiments in th edeep North Atlantic: isotopic and elemental f1uxes", J. Mar. Res., Vol. 38, pp. 703-728.
  13. Deuser, W.G. and Ross, E.H.(1980), "Seasonal changes in the f1ux of organic carbon to the deep Sargasso Sea", Nature, Vol. 283, pp. 364-365. https://doi.org/10.1038/283364a0
  14. Deuser, W.G., Ross, E.H., and Anderson, R.F.(1981), "Seasonality in the supply of sediment to the deep Sargasso Sea and implication for the rapid transfer of matter to the deep ocean", Deep-Sea Res., Vol. 28, pp. 495-505. https://doi.org/10.1016/0198-0149(81)90140-0
  15. Dugdale, R.C. and Goering, J.(1967). "Uptake of new and regenerated forms of nitrogen in primary productivity" Limnol. Oceanogr., Vol. 12, pp. 196-206. https://doi.org/10.4319/lo.1967.12.2.0196
  16. Dunbar, R.B.(1984). "Sediment trap experiments on the Antarctic continental margin", Antarctic J. U. S., Ann. Rev. Iss., Vol. 19, pp. 70-71.
  17. Dymond, J., Fisher, K., Clauson, M., Cobler, R., Gardner, W., Berger, W., Richardson, M., Soutar, A., and Dunbar, R.(1981), "A sediment trap intercomparison study in the Santa Barbara basin", Earth Planet, Sci. Lett., Vol. 53, pp. 409-418. https://doi.org/10.1016/0012-821X(81)90045-5
  18. El-Sayed, S.Z.(1968), "Primary productivity of the Antarctic and subantarctic", pp. 119-135. In: Primary Productivity and Benthic Marine Algae of the Antarctic and Subantarctic. Antarctic Map Folio series, Folio 10.(V. Bushnell, ed.). American GeographicaI Society, New York.
  19. El-Sayed, S.Z.(1970), "On the productivity of the Southern Ocean (Atlantic and Pacific sectors)", pp. 119-135. In: Antarctic Ecology (A. Holdgate, ed.). Vol 1. Academic Press, New York.
  20. El-Sayed, S.Z. and Turner, J.T.(1977), "Productivity of the Antarctic and tropical subtropical regions: A comparative study", pp. 463-504. In: Polar Oceans (M.J. Dunbar, ed.). Arctic Institute of North America, Calgary, Alberta, Canada.
  21. El-Sayed, S.Z. and Taguchi, S.(1981), "Primary production and standing crop of phytoplankton along the ice-edge in the Weddell Sea", Deep-Sea Res., Vol. 28A, No. 9, pp. 1017-1032.
  22. El-Sayed, S.Z. and Weber, L.H.(1982), "Spatial and primary productivity in the southwest Atlantic and the Scotia Sea", Polar Biol., Vol. 1, pp. 83-90.
  23. Eppley, R.W. and Peterson, B.J.(1979), "Particulate organic matter flux and planktonic new production in the deep ocean", Nature, 282, pp. 677-680. https://doi.org/10.1038/282677a0
  24. Eppley, R.W., Renger E.H., and Betzer P.R.(1983), "The residence time of particulate organic carbon in the surface layer of the ocean", Deep-Sea Res., Vol. 30, pp. 311-323. https://doi.org/10.1016/0198-0149(83)90013-4
  25. Evans, C. A., O'Reilly, J. E., and Thomas, J.P.(1987), "A Handbook for the Measurement of Chlorophyll a and Primary Production", BIOMASS Scientific Series No. 8. 114pp, Texas A&M Univ., College Stadon, Texas.
  26. Fogg, G.E.(1977), "Aquatic primary production in the Antarctic", Phil. Trans R. Soc. Lond. Vol. B 279, pp. 27-38.
  27. Gallagher, J.C., Wood, A.M., and Alberte, R.S.(1984), "Ecotyptic differentiation in the marine diatom Skeletonema costatum: influence of light intensity on the photosynthetic apparatus", Mar. Biol., Vol. 82, pp. 121-134. https://doi.org/10.1007/BF00394096
  28. Hayes, P.K., Whitaker, T.M., and Fogg, G.E.(1984), "The distribution and nutrient status of phytoplankton in the Southern Ocean between 20 and 70 $^{\circ}W$", Polar Biol., Vol. 3, pp. 153-165. https://doi.org/10.1007/BF00442647
  29. Holm-Hansen, O.(1985), "Nutrient cycles in Antarctic Marine Ecosystems", pp. 6-10. In: Antarctic Nutrient Cycles and Food Webs (W. R. Siegfried, P.R. Condy, and R. M. Laws, eds.). Springer-Verlag, Berlin.
  30. Holm-Hansen, O., El-Sayed, S.Z., Franceschini, G.A., and Cuhel, R.L.(1977), "Primary production and the factors controlling phytoplankton growth in Southern Ocean", pp. 11-50. In: Adaptations within Antarctic Ecosystems; Proceedings of the third SCAR Symposium on Antarctic Biology (G.A. Llano, ed.). National Academy of Sciences, Washington, D.C.
  31. Honjo, S.(1978), "Sedimentation of materials in the Sargasso Sea at a 5367 m deep station", J. Mar. Res., Vol. 36, pp. 469-492.
  32. Honjo, S.(1982), "Seasonality and interaction of biogenic and lithogenic particulate flux at the Panama Basin", Science, Vol. 218, pp. 883-884 https://doi.org/10.1126/science.218.4575.883
  33. Jeffrey, S.W.(1968), "Quantitative thin-layer chromatography of chlorophylls and carotenoids from marine algae", Biochim. Biophys. Acta, Vol 162, pp. 271-285. https://doi.org/10.1016/0005-2728(68)90109-6
  34. Jeffrey, S.W.(1972), "Preparation and some properties of crystalline chlorophyll $c_1\;and\;c_2$ from marine algae", Biochim. Biophys. Acta, Vol. 279, pp. 15-33. https://doi.org/10.1016/0304-4165(72)90238-3
  35. Jeffrey, S.W.(1974), "Profiles of photosynthetic pigments in the ocean using thin-layer chromatography", Mar. Biol., Vol. 26, pp. 101-110. https://doi.org/10.1007/BF00388879
  36. Jeffrey, S.W.(1981), "An improved thin-layer chromatographic technique for marine phytoplankton pigments", Limnol. Oceanogr., Vol. 16, pp. 191-197.
  37. Jeffrey, S.W. and Humphrey, G.F.(1975), "New spectrophotometric equations for determining chlorophylls a, b, $c_1\;and\;c_2$ in higher plants, algae and natural phytoplankton", Biochemie und Physiologie der Pflanzen, Vol. 167, pp. 191-194.
  38. Jeffrey, S.W. and Hallegraeff, G.M.(1980), "Studies on phytoplankton species and photosynthetic pigments in a warm core eddy of the East Austrailian Current. I. Summer populations", Mar. Ecol. Prog. Ser., Vol. 3, pp. 285-294. https://doi.org/10.3354/meps003285
  39. Jennings, J.C., Gordon, L.I., and Nelson, D.M.(1984), "Nutrient depletion indicates high primary productivity in the Weddell Sea", Nature, Vol. 309, pp. 51-54 https://doi.org/10.1038/309051a0
  40. Karl, D.M., Knauer, G.A., Martin, J.H., and Ward, B.B.(1984), "Chemolithotrophic bacterial production in association with rapid sinking particles: Implication of oceanic carbon cycles and mesopelagic food webs", Nature, Vol. 309, pp. 54-56. https://doi.org/10.1038/309054a0
  41. Karl, D.M., G. A. Knauer, and Martin, J.H.(1988), "Downward flux of particulate organic matter in the ocean:a particle decomposition paradox", Nature, Vol 332, pp. 438-441. https://doi.org/10.1038/332438a0
  42. Knauer, G.A., Hebel, D., and Cippriano, F.(1982), "Marine snow: Major site of primary production in coa.stal waters", Nature, Vol. 300, pp. 630-631. https://doi.org/10.1038/300630a0
  43. Knauer, G.A., Redalje D.G., Harrison W.G. and Karl, D.M.(1990), "New production at the VERTEX time-series site", Deep-Sea Res., Vol. 37(7), pp. 1121-1134 https://doi.org/10.1016/0198-0149(90)90054-Y
  44. Lorenzen, C.J.(1972), "Extinction of light in the ocean by phytoplankton", J. Cons. Int. Explor. Mer., Vol. 34, pp. 262-267. https://doi.org/10.1093/icesjms/34.2.262
  45. Lorenzen, C.J.(1976), "Primary production in the sea", pp. 173-185. In: Ecology of the Seas (D. H. Cushing and J. J. Walsh, eds.). Blackwell Scientific Publications, Oxford.
  46. Mantoura, R.F. and Llewellyn, C.A.(1983), "The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography", Anal. Chim. Acta, Vol. 151, pp. 297-314. https://doi.org/10.1016/S0003-2670(00)80092-6
  47. Marra, J., Houghton, R.W., Boardman, D.C., and Neale, P.J.(1982), "Variability in surface chlorophyll a at a shelf break front", J. Mar. Res., Vol. 40, pp. 575-591.
  48. McCave, I. N.(1975), "Vertical flux of particles in the ocean", Deep-Sea Res., Vol. 22, pp. 491-502.
  49. Noh. I.(1996), "Algal pigments and their degradation products in suspended and sinking particulate material in the Gulf of Mexico", Journal of the Korean Environmental Sciences Society, Vol. 5(2), pp 153-170.
  50. Noh, I.(2011 in press), "Carotenoid pigments from suspended and sinking particulate matter in Prydz Bay, Antarctica", Journal of the Korean Environmental Sciences Society.
  51. Parsons, T.R., Stronach, J., Borstad, G.A., Louttit, G., and Petty, R.I.(1981), "Biological fronts in the Strait of Georgia, British Colombia", Mar. Ecol. Prog. Ser., Vol. 6, pp. 237-242. https://doi.org/10.3354/meps006237
  52. Rowe, G.T. and Gardner, W.D.(1979), "Sedimentation rates in the slope water of the northwest Atlantic Ocean measured directly with sediment traps", J. Mar. Res., Vol. 37, pp. 581-600.
  53. Sakshaug, E. and Holm-Hansen, O.(1984), "Factors governing pelagic production in po1ar oceans", pp. 1-17. In: Marine Phytoplankton and Productivity (L. Bolis and R. Gilles, eds.). Springer-Verlag, Berlin
  54. Seliger, H.H., McKinley, K.R., Biggley,, W.H., Rivkin, R.B., and Aspden, K.R.H.(1981), "Phytoplankton patchiness and frontal regions", Mar. Biol., Vol. 61, pp. 119-131. https://doi.org/10.1007/BF00386651
  55. Shanks, A.L. and Trent, J.D.(1979), "Marine Snow: Microscale nutrient patches", Limnol. Oceanogr., Vol. 24, pp. 850-854. https://doi.org/10.4319/lo.1979.24.5.0850
  56. Silver, M.W., Shanks, A.L., and Trent, J.D.(1978), "Marine snow: microplankton habitat and sources of small-scale patchiness in pelagic populations", Science, Vol. 201, pp. 371-373. https://doi.org/10.1126/science.201.4353.371
  57. Smith, W.O.Jr., and Nelson, D.M.(1985), "Phytoplankton bloom produced by a receding ice edge in the Ross Sea: Spatial coherence with the density field", Science, Vol. 227, pp. 163-166. https://doi.org/10.1126/science.227.4683.163
  58. Steele, J.H.(1962), "Environmental control of photosynthesis in the sea", Limnol. Oceanogr., Vol. 7, pp. 137-150. https://doi.org/10.4319/lo.1962.7.2.0137
  59. Stephens, F.C.(1989), "Effects of ultraviolet light on photosynthesis and pigments of Antarctic marine phytoplankton", PhD. Dissertation, Texas A&M University, 121pp.
  60. Strickland, J.D.H. and Parsons, T.R.(1972), "A Practical Handbook of Seawater Analysis", Fish. Res. Board Can. Bull. 167, 2nd ed. Ottawa, Canada.
  61. Suess, E.(1980), "Particulate organic carbon flux in the oceans: surface productivity and oxygen utilization", Nature, Vol. 288, pp. 260-263. https://doi.org/10.1038/288260a0
  62. Tilzer, M.M., von Bodungen, B., and Smetacek, V.(1985), "Light-dependence of phytoplankton photosynthesis in the Antarctic Ocean: Implications for regulating productivity", pp. 60-69. In: Antarctic Nutrient Cycles and Food Webs (W. R. Siegfried, P. R. Condy, m1d R. M. Laws, eds.). Springer-Verlag, Berlin.
  63. Tilzer, M.M., Elbraechter, M., Gieskes, W.W., and Beese, B.(1986), "Light-temperature interactions in the control of photosynthesis in Antarctic phytoplankton", Polar Biol., Vol. 5, pp. 105-111. https://doi.org/10.1007/BF00443382
  64. Trent, J.D., Shanks, A.L., and Silver, M.W.(1978), "In situ and laboratory measurements on macroscopic aggregates in Monterey Bay, California", Limnol. Oceanogr., Vol. 23, pp. 626-635. https://doi.org/10.4319/lo.1978.23.4.0626
  65. Urrere, M.A. and Knauer, G.A.(1981), "Zooplankton fecal pellet fluxes and vertical transport of particulate organic material in the pelagic environment", J. Plank. Res., Vol. 3, pp. 369-387. https://doi.org/10.1093/plankt/3.3.369
  66. Weber, L.H. and El-Sayed, S.Z.(1985), "Spatial variability of phytoplankton and the distribution and abundance of krill in the Indian sector of the Southern Ocean", pp. 284-293. In: Antarctic Nutrient Cycles and Food Webs (W. R. Siegfried, P. R. Condy, and R. M. Laws, eds.). Springer-Verlag, Berlin.
  67. Welschmeyer, N.A. and Lorenzen, C.J.(1985), "Chlorophyll budgets: zooplankton grazing and phytoplankton growth in a temperate fjord and the central pacific gyres", Limnol. Oceanogr., Vol. 30, pp. 1-21. https://doi.org/10.4319/lo.1985.30.1.0001

피인용 문헌

  1. Carotenoid Pigments from Suspended and Sinking Particulate Matter in Prydz Bay, Antarctica vol.20, pp.11, 2011, https://doi.org/10.5322/JES.2011.20.11.1357