Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characterization of ERp29 and ADP-Ribosylation Factor 5 Interaction

ERp29와 ADP-ribosylation factor 5의 결합특성

  • Kwon, Ki-Sang (Department of Anatomy, School of Medicine, Chungnam National University) ;
  • Seog, Dae-Hyun (Department of Biochemistry, College of Medicine, Inje University) ;
  • Kim, Seung-Whan (Department of Emergency Medicine, Chungnam National University Hospital) ;
  • Yu, Kweon (Aging Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, O-Yu (Department of Anatomy, School of Medicine, Chungnam National University)
  • 권기상 (충남대학교 의학전문대학원 해부학교실) ;
  • 석대현 (인제대학교 의과대학 생화학교실) ;
  • 김승환 (충남대학교병원 응급의학과) ;
  • 유권 (한국생명공학연구원 장수연구센타) ;
  • 권오유 (충남대학교 의학전문대학원 해부학교실)
  • Received : 2011.03.28
  • Accepted : 2011.04.25
  • Published : 2011.04.30
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Abstract

ERp29 is a endoplasmic reticulum (ER) lumenal resident protein that shows sequence similarity to the protein disulfide isomerase family. Its biological function is thought to play a role in the processing of secretory proteins within the ER, possibly by participating in the folding of proteins in the ER. Although some data on ERp29 have been reported, its normal functions are still unclear. To gain insights into the function of ERp29, we identified ARF5 protein as a protein that interacts with ERp29 using yeast two-hybrid screening and GST pull-down assay. Interaction between ERp29 and ARF5 was detected under normal cell conditions but not under ER stress conditions. This result may provide a clue for understanding ERp29 biological functions.

ERp29는 endoplasmic reticulum (ER) lumen에 존재하는 단백질로 protein disulfide isomerase (PDI) family에 속한다. 비록 관련 연구 결과는 조금 있지만 정확한 생물학적인 기능은 아직 분명하지 않지만, 분비단백질과정과 단백질 folding에 관여하는 것으로 알려 지고 있다. ERp29의 기능 연구를 위하여 yeast two-hybrid screening/GST pull-down assay방법을 사용하여 ERp29-결합단백질인 ADP-ribosylation factor 5 (ARF5)를 동정하였다. 이들의 결합은 정상적인 세포생리상태에서는 결합하지만 ER stress 상태에서는 떨어졌다. 이 결과는 ERp29의 기능 연구를 위하여 하나의 실마리를 제공할 것이다.

Keywords

References

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