생명과학회지 (Journal of Life Science)

  • 제21권4호
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  • Pages.589-595
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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신규 분리균주 Streptomyces sp. BCNU 1030의 in vitro 항균활성

In vitro Antimicrobial Activity of a New Isolate Streptomyces sp. BCNU 1030

  • 방지훈 (창원대학교 생명공학협동과정 및 생물학과) ;
  • 최혜정 (창원대학교 생명공학협동과정 및 생물학과) ;
  • 안철수 (조아제약(주)) ;
  • 김동완 (창원대학교 미생물학과) ;
  • 정영기 (동아대학교 생명공학과) ;
  • 주우홍 (창원대학교 생명공학협동과정 및 생물학과)
  • Bang, Ji-Hun (Interdisciplinary Program in Biotechnology and Department of Biology, Changwon National University) ;
  • Choi, Hye-Jung (Interdisciplinary Program in Biotechnology and Department of Biology, Changwon National University) ;
  • Ahn, Cheol-Soo (Cho-A Pharm. Co, LTD.) ;
  • Kim, Dong-Wan (Department of Microbiology, Changwon National University) ;
  • Jeong, Yong-Kee (Department of Biotechnology, Dong-A University) ;
  • Joo, Woo-Hong (Interdisciplinary Program in Biotechnology and Department of Biology, Changwon National University)
  • 투고 : 2011.02.22
  • 심사 : 2011.03.01
  • 발행 : 2011.04.30
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초록

질병를 유발하는 미생물들의 항생물질에 대한 증가하는 내성문제를 극복할 수 있는 새로운 항생물질을 개발하고자 항생물질을 생산하는 방선균을 탐색하여 특성화하는데 본 연구는 초점을 맞추고 있다. 충청북도 일대의 토양으로부터 PDA, starch casein agar 등의 배지를 이용하여 100여 개의 방선균주를 순수분리하였다. 순수분리된 방선균주 중 메티실린 내성 황색포도상구균에 가장 활성이 뛰어난 BCNU 1030을 선발하여 형태학적, 생화학적, 분자생물학적 방법으로 동정한 결과 Streptomyces 속으로 동정되었다. 분리균주인 BCNU 1030은 MRSA를 포함한 그람양성 및 그람음성세균에 대한 폭넓은 항균스펙트럼을 가졌다. BCNU 1030의 디클로로메탄 추출물은 MRSA CCARM 3090에 대하여 최소$0.78\;{\mu}g/ml$의 농도에서 저해활성이 있음이 확인되었다. 그러므로 BCNU 1030은 새로운 anti-MRSA 항생물질의 개발에 있어서 가능성을 가지고 있는 것으로 판단된다.

This work focused on screening and characterizing antibiotic-producing actinomycetes to develop new antibiotics that can overcome the growing resistance of disease-causing microbes. One-hundred actinomycetes strains were isolated from soil samples from Chungcheongbuk-do, Korea using various kinds of actinomycetes isolation media, including a starch casein agar medium and potato dextrose agar (PDA). Among them, strain BCNU 1030 was determined to show strong antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). Biochemical, physiological, and 16S rRNA sequence analyses indicated that strain BCNU 1030 belonged to the genus Streptomyces. Strain BCNU 1030 exhibited antibiotic activity against a wide range of bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of BCNU 1030 dichloromethane extract was determined to be $0.78\;{\mu}g/ml$ for MRSA CCARM 3090. Therefore, Streptomyces sp. BCNU 1030 has potential for anti-MRSA drug development.

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