생명과학회지 (Journal of Life Science)

  • 제21권3호
  • /
  • Pages.358-367
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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DOI QR코드

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대두부산물의 지방세포분화 유도유전자의 발현저해 및 전지방세포 분화 억제 효과

Suppressive Effects of By-Product Extracts from Soybean on Adipocyte Differentiation and Expression of Obesity-Related Genes in 3T3-L1 Adipocytes

  • Choi, Mi-Sun (Department of Biological Science, Andong National University) ;
  • Kim, Jee-In (Department of Biological Science, Andong National University) ;
  • Jeong, Jin-Boo (Department of Medicinal Plant Resource, Andong National University) ;
  • Lee, Su-Bok (Department of Biological Science, Andong National University) ;
  • Jeong, Jae-Nam (Department of Biological Science, Andong National University) ;
  • Jeong, Hyung-Jin (Department of Medicinal Plant Resource, Andong National University) ;
  • Seo, Eul-Won (Department of Biological Science, Andong National University) ;
  • Kim, Taek-Yoon (Quality Control Section, Hahoefood Co.) ;
  • Kwon, Oh-Jun (Industrial Evaluation Agency for Gyeongbuk Province) ;
  • Lim, Jae-Hwan (Department of Biological Science, Andong National University)
  • 투고 : 2010.10.27
  • 심사 : 2010.12.09
  • 발행 : 2011.03.30
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초록

대두는 여러 종류의 파이토케미컬을 함유하고 있으며 이에 의한 항산화, 항염증, 항비만 효능 등이 있음이 잘 알려져 있다. 대두 부산물 성분의 비만억제효능을 확인하기 위하여 Oil-Red O 염색법과 정량 PCR을 통하여 지방세포분화억제 및 지방생성억제 효능을 분석하였다. 분화유도물질인 isobutylmethylanthine (IBMX), dexamathasone 및 insulin 처리에 따른 지방세포 분화유도와 함께 대두부산물-침지수, 순물, 두유-의 처리는 세포손상 없이 지방세포분화 연관 유전자-PPAR${\gamma}$, Fabp4, Scd1, adipsin, apolipoprotein (APOE), adiponectin (ADIPOQ)-의 발현을 감소시키는 효능을 보였다. 또한 대두에서 잘 알려진 isoflavone -daidzein과 genistein- 분석을 통하여 대두 부산물에 포함된 두 종류의 isoflavone의 함량을 확인하였다. 결과적으로 대두부산물에는 지방세포분화유도 유전자의 발현억제를 통한 전지방세포의 분화 및 지방세포의 지방생성을 억제하고 있음을 확인하였다. 따라서 두부생산 시 발생하는 대두부산물은 지방세포분화 억제 효능을 바탕으로 이를 이용하는 건강기능 식품제조에 활용될 수 있을 것으로 판단된다.

Soybean is known to contain various phytochemicals that are related to anti-oxidant, anti-inflammatory and anti-obesity effects in mice and humans. The anti-obesity effect of by-product extracts from soybean on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil Red-O assay and quantitative PCR. In inducing differentiation of 3T3-L1 pre-adipocytes in the presence of an adipogenic cocktail, isobutylmethylanthine (IBMX), dexamathasone, and insulin, treatment with filtrated soybean soaked water, soybean milk, and soycurd residue from soybean curd processing significantly decreased mRNA expression of obesity-related gene such as PPAR${\gamma}$, Fabp4, and Scd1, adipsin, apolipoprotein (APOE) and adiponectin (ADIPOQ) without any significant cytotoxicity. We also determined the well-known isoflavones in soybean, such as daidzein and genistein, in the by-product extracts. Taken together, we suggest that soybean by-product extract showed anti-obesity effect by suppressing adipocyte related gene expression, and that by-products collected during soybean curd processing may be a good candidate as an ingredient in health care products.

키워드

참고문헌

  1. Adlercreutz, H., Y. Mousavi, J. Clark, K. Hockerstedt, E. Hamalainen, K. Wahala, T. Makela, and T. Hase. 1992. Dietary phytoestrogens and cancer: in vitro and in vivo studies. J. Steroid Biochem. Mol. Biol. 41, 331-337. https://doi.org/10.1016/0960-0760(92)90359-Q
  2. Ahn, I. S., K. Y. Pack, and M. S. Do. 2007. Weight control mechanisms and antiobesity functional agents. J. Korean Soc. Food Sci. Nutr. 36, 503-513. https://doi.org/10.3746/jkfn.2007.36.4.503
  3. Anne, W. H. and B. H. Joyce. 2001. Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis. Am. J. Physiol. Cell Physiol. 280, C807-C813.
  4. Bae, E. A., T. W. Kwon, and G. S. Moon. 1997. Isoflavone contents and antioxidative effects of soybean, soybean curd and their by-products. J. Korean Soc. Food Sci. Nutr. 26, 371-375.5.
  5. Barnes, S., H. Kim, T. G. Peterson, and J. Xu. 1998. Isoflavones and cancer-the estrogen paradox. Korean Soybean Digest 15, 81-93.
  6. Bondet. V., W. Brand-Williams, and C. Berset. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. Food Sci. Technol. 30, 609-615.
  7. Brun, R. P. and B. M. Spiegelman. 1997. PPAR gamma and the molecular control of adipogenesis. J. Endocrinol. 155, 217-218. https://doi.org/10.1677/joe.0.1550217
  8. Chin, H. S., K. J. Pack, S. H. Pack, and J. K. Kim. 2009. The effects of herbal extract mixture on anti-obesity. J. Korean Soc. Food Sci. Nutr. 38, 32-38. https://doi.org/10.3746/jkfn.2009.38.1.032
  9. Choi, J. S., T. W. Kwon, and J. S. Kim. 1996. Isoflavone contents in some varieties of soybean. Foods Biotechnol. 5, 167-167.
  10. Choi, Y. B., K. S. Kim, and H. S. Sohn. 1995. Recovery of soy-oligosaccharides using calcium oxide. J. Food Sci. 27, 225-229.
  11. Cook, K. S., D. L. Groves, H. Y. Min, and B. M. Spiegelman. 1985. A developmentally regulated mRNA from 3T3 adipocytes encodes a novel serine protease homologue. Proc. Natl. Acad. Sci. USA 82, 6480-6484. https://doi.org/10.1073/pnas.82.19.6480
  12. Dnizot, F. D. and L. Rita. 1986. Rapid colorimetric assay for cell growth and survival: Modification to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 22, 271-277.
  13. Flier, J. S. and E. Maratos-Flier. 1998. Obesity and the hypothalamus: novel peptides for new pathways. Cell 92, 437-440. https://doi.org/10.1016/S0092-8674(00)80937-X
  14. Fruhbeck, G., J. Gomez-Ambrosi, F. J. Muruzabal, and M. A. Burrell. 2001. The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation. J. Physiol. Endocrinol. Metab. 280, E827-E847.
  15. Galvez, A. F. and B. O. de Lumen. 1999. A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nat. Biotechnol. 17, 495-500. https://doi.org/10.1038/8676
  16. Gregoire, F. M., C. M. Smas, and H. S. Sul. 1998. Understanding adipocyte differentiation. Physiol. Rev. 78, 783-809.
  17. Hackler, L. R. and B. R. Stillings. 1967. Amino acid compostion of heat-processed soymilk and its correlation with nutritive value. Cereal. Chem. 44, 70-77.
  18. Harold, E. S., E. A. Darrell, I. F. Evan, and A. M. John. 2007. A review of the interaction among dietary antioxidants and reactive oxygen species. J. Nutr. Biochem. 18, 567-579. https://doi.org/10.1016/j.jnutbio.2006.10.007
  19. Hwang, J., J. Wang, P. Morazzoni, H. N. Hodis, and A. Sevanian. 2003. The phytoestrogen equol increases nitric oxide availability by inhibiting superoxide production : an antioxidant mechanism for cell-mediated LDL modificarion. Free Radic. Biol. Med. 34, 1271-1282. https://doi.org/10.1016/S0891-5849(03)00104-7
  20. Kaufman, P. B., J. A. Duke, H. Brielmann, J. Boik, and J. E. Hoyt. 1997. A comparative survey of leguminous plants as sources of the isoflavones, genistein and daidzein: implications for human nutrition and health. J. Altern. Complement Med. 3, 7-12. https://doi.org/10.1089/acm.1997.3.7
  21. Kim, K. S., H. K. Chun, and H. S. Sohn. 1994. Purification of oligosaccharides from soybean using activated charcoal. Korean J. Food Sci. Technol. 3, 156-156.
  22. Kim, Y. J., B. H. Kim, S. Y. Lee, M. S. Kim, C. S. Pack, M. S. Rhee, K. H. Lee, and D. S. Kim. 2006. Screening of medicinal plants for development of functional food ingredients with anti-obesity. J. Korean Soc. Appl. Biol. Chem. 49, 221-226.
  23. Lau, D. C., B. Dhillon, H. Yan, P. E. Szmitko, and S. Verma. 2005. Adipokines: molecular links between obesity and atheroslcerosis. Am. J. Physiol. Heart Circ. Physiol. 288, 2031-2041. https://doi.org/10.1152/ajpheart.01058.2004
  24. Lee, C. H., P. Olson, and R. M. Evans. 2003. Minireview: lipid metabolism, metabolic diseases, and peroxisome proliferator-activated receptors. Endocrinology 144, 2201-2207. https://doi.org/10.1210/en.2003-0288
  25. MacDougald, O. A. and M. D. Lane. 1995. Transcriptional regulation of gene expression during adipocyte differentiation. Annu. Rev. Biochem. 64, 345-373. https://doi.org/10.1146/annurev.bi.64.070195.002021
  26. Mohamed-Ali, V., J. H. Pinkney, and S. W. Coppack. 1998. Adipose tissue as an endocrine and paracrine organ. Int. J. Obes. Relat. Metab. Disord. 22, 1145-1158. https://doi.org/10.1038/sj.ijo.0800770
  27. Potter, S. M. 1995.Overview of proposed mechanisms for the hypocholesterolemic effect of soy. J. Nutr. 125, 606S-611S.
  28. Ramirez-Zacarias, J. L., F. Castro-Munozledo, and W. Kuri-Harcuch. 1992. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97, 493-497. https://doi.org/10.1007/BF00316069
  29. Rosen, E. D. and O. A. MacDougald. 2006. Adipocyte differentiation from the inside out. Nat. Rev. Mol. Cell Biol. 7, 885-896. https://doi.org/10.1038/nrm2066
  30. Rosen, E. D., C. J. Walkey, P. Puigserver, and B. M. Spiegelman. 2000. Transcriptional regulation of adipogenesis. Genes Dev. 14, 1293-1307.
  31. Sharma, O. P., H. Adlercreutz, J. D. Strandberg, B. R. Zirkin, D. S. Coffey, and L. L. Exing. 1992. Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats. J. Steroid Biochem. Mol. Biol. 43, 557-564. https://doi.org/10.1016/0960-0760(92)90244-D
  32. Spiegelman, B. M. and J. S. Flier. 2001. Obesity and the regulation of energy balance. Cell 104, 531-543. https://doi.org/10.1016/S0092-8674(01)00240-9
  33. Steppan, C. M., S. T. Bailey, S. Bhat, E. J. Brown, R. R. Banerjee, C. M. Wright, H. R. Patel, R. S. Ahima, and M. A. Lazar. 2001. The hormone resistin links obesity to diabetes. Nature 409, 307-312. https://doi.org/10.1038/35053000
  34. Wei, H., L. Wei, F. Frenkel, R. Bowen, and S. Barnes. 1993. Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein. Nutr. Cancer 20, 1-20. https://doi.org/10.1080/01635589309514265
  35. Weiss, L. G., R. Hoffmann, E. Schreiber, H. Andres, E. Fuchs, and H. J. Kolb. 1986. Fatty-acid biosynthesis in man, a pathway of minor importance. Purification, optimal assay conditions, and organ distribution of fatty-acid synthesis. Biol. Chem. Hoppe Seyler. 367, 905-912. https://doi.org/10.1515/bchm3.1986.367.2.905

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