생명과학회지 (Journal of Life Science)

  • 제21권3호
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  • Pages.335-340
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Saccharomyces cerevisiae와 Pichia stipitis를 이용한 오탄당과 육탄당으로부터 에탄올 생산에 관한 비교연구

Comparative Study on Ethanol Production with Pentose and/or Hexose by Saccharomyces cerevisiae and/or Pichia stipitis

  • 김중곤 (부산대학교 나노융합기술학과) ;
  • 안정훈 (KAIST 부설 한국과학영재학교)
  • 투고 : 2010.10.20
  • 심사 : 2011.02.18
  • 발행 : 2011.03.30
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초록

포도당과 자일로스는 자연계에서 가장 풍부한 물질이며 이들은 효모에 의해 에탄올로 전환될 수 있다. 본 연구에서는 Saccharomyces cerevisiae와 Pichia stipitis을 이용하여 분리배양, 공동배양 그리고 순차배양 등의 조합을 통해 가장 효과적인 발효의 방법을 찾고자 하였다. 분리배양에서 S. cerevisiae은 29.4 g/l의 포도당을 발효하여 14.5 g/l의 에탄올을 생산한 반면에 자일로스를 이용하지 못했다. 그렇지만 P. stipitis은 포도당뿐만 아니라 자일로스도 분해하여 각각 포도당 29.4 g/l로부터 11.9 g/l의 에탄올을, 자일로스 29.0 g/l로부터 11.6 g/l의 에탄올을 생산하였다. 포도당과 자일로스 혼합배양에서, S. cerevisiae은 13.4 g/l의 에탄올을 생산한 반면에 P. stipitis은 21.1 g/l의 에탄올을 생산하였다. 공동배양과 순차배양에서, 공동배양이 18.6 g/l, 순차배양이 12.4 g/l의 에탄올을 생산하여 공동배양이 더 효과적인 것으로 나타났다. 두 효모의 생장에서 영양분의 효과를 보기 위해 yeast nitrogen base (YNB)을 S. cerevisiae가 포도당을 소모한 시점에 첨가하니 자일로스의 소비량과 미생물의 성장이 증가하였고 54.6 g/l의 당 혼합배양액에서 22.5 g/l의 에탄올을 생산하여 0.41 g/g의 수득율을 나타내었다.

Glucose and xylose are the most abundant materials in nature which can be used to produce ethanol by yeast fermentation. Three combinations of cultivation with glucose and xylose were carried out; separated, co-culture, and sequential fermentation with Saccharomyces cerevisiae and Pichia stipitis. In the separated fermentation, S. cerevisiae fermented glucose to produce 14.5 g/l ethanol from 29.4 g/l glucose but hardly used xylose. However, P. stipitis utilized not only glucose but also xylose to produce ethanol 11.9 g/l and 11.6 g/l from 29.4 g/l glucose and 29.0 g/l xylose, respectively. In the mixture of glucose and xylose, P. stipitis fermented both sugars, producing 21.1 g/l ethanol while S. cerevisiae fermented only glucose, producing 13.4 g/l ethanol. In the co-culture and sequential fermentation, the co-culture showed more efficient ethanol productivity with 18.6 g/l ethanol than the sequential fermentation with 12.4 g/l ethanol. To investigate the effect of nutrients in the growth of microorganisms and ethanol production, yeast nitrogen base (YNB) was used in the sequential fermentation with S. cerevisiae and P. stipitis. YNB supplemented some nutrients which S. cerevisiae used up in the broth and the culture showed increased growth rate, increased consumption of xylose, and increased ethanol productivity producing 22.5 g/l ethanol from 54.6 g/l sugar with a yield of 0.41 g/g.

키워드

참고문헌

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