Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Study on the Anti-inflammatory Effect of Yeonguemjiri-tang Water Extract

연금지리탕(連芩止痢湯) 물 추출물의 항염증작용에 관한 연구

  • Kim, Jung-Hwan (Department of Preventive Medicine, College of Oriental Medicine) ;
  • Lee, Jang-Suk (Department of Preventive Medicine, College of Oriental Medicine) ;
  • Kang, Ok-Hwa (Department of Oriental Pharmacy, College of Pharmacy) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy) ;
  • Lee, Ki-Nam (Department of Preventive Medicine, College of Oriental Medicine) ;
  • Chong, Myong-Soo (Department of Preventive Medicine, College of Oriental Medicine)
  • 김정환 (원광대학교 한의과대학 예방의학교실) ;
  • 이장석 (원광대학교 한의과대학 예방의학교실) ;
  • 강옥화 (원광대학교 약학대학 한약학과) ;
  • 권동열 (원광대학교 약학대학 한약학과) ;
  • 이기남 (원광대학교 한의과대학 예방의학교실) ;
  • 정명수 (원광대학교 한의과대학 예방의학교실)
  • Received : 2011.11.18
  • Accepted : 2011.12.15
  • Published : 2011.12.25
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Abstract

Yeonguemjiri-tang(連芩止痢湯, YGT) exhibits potent anti-inflammatory activity in widely intestine disease, but its mechanism undisclosed. To elucidate the molecular mechanisms of YGT on pharmacological and biochemical actions in inflammation, we examined the effect of YGT on pro-inflammatory mediators in phorbol 12-myristate 13-acetate (PMA) plus A23187-induced mast cell and lipopolysaccharide (LPS)-stimulated macrophages. The investigation focused on whether YGT inhibited pro-inflammatory cytokines such as interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in PMA plus A23187-induced HMC-1 cells and inflammatory madiators such as nitric oxide (NO), TNF-${\alpha}$, IL-6, iNOS, COX-2 in LPS-stimulated RAW 264.7 cells. We found that YGT inhibited LPS-induced NO, TNF-${\alpha}$ and IL-6 productions as well as the expressions of iNOS and COX-2. These results suggest that YGT has inhibitory effects on mast cell-mediated and macrophage-mediated inflammation.

Keywords

References

  1. 李兆華 編著. 傳染病證治從新, 河北人民出版社, 1981.
  2. 신민교. 임상본초학, 서울, 영림사, pp 280-282, 387, 400-404, 417-418, 487, 488, 598-600, 658-660, 1997.
  3. 王崇國 主編. 臨床辨病傳方治療從書, 傳染病, 辨病傳方治療, 人民衛生出版社, p 246, 2000.
  4. Higuchi, M., Hisgahi, N., Taki, H., Osawa, T. Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-argininedependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. J Immunol 144: 1425-1431, 1990.
  5. Kim, K.M., Yu, K.W., Kang, D.H., Koh, J.H., Hong, B.S., Suh, H.J. Anti-stress and anti-fatigue effects of fermented rice bran. Biosci Biotechnol Biochem 65(10):2294-2296, 2001. https://doi.org/10.1271/bbb.65.2294
  6. Stamler, J.S., Singel, D.J., Loscalzo, J. Biochemistry of nitric oxide and its redox-activated forms. Science Dec; 18. 258(5090):1898-1902, 1992. https://doi.org/10.1126/science.1281928
  7. Ehrlich, P. Beitrage zur kenntnis der Anilinfarbunger und ihrer Verwendung in drmikroskopischen Technik. Arch Mikrosk Anast 13: 263-277, 1877. https://doi.org/10.1007/BF02933937
  8. Kim, J.A., Kim, D.K., Kang, O.H., Choi, Y.A., Park, H.J., Choi, S.C., Kim, T.H., Yun, K.J., Nah, Y.H., Lee, Y.M. Inhibitory effect of luteolin on TNF-alpha-induced IL-8 production in human colon epithelial cells. Int Immunopharmacol 5(1):209-217, 2005. https://doi.org/10.1016/j.intimp.2004.09.027
  9. Mahida, Y.R., Ceska, M., Effenberger, F., Kurlak, L., Lindley, I., Hawkey, C.J. Enhanced synthesis of neutrophil-activating peptide-1/interleukin-8 in active ulcerative colitis. Clin Sci (Lond) Mar; 82(3):273-275, 1992. https://doi.org/10.1042/cs0820273
  10. Izzo, R.S., Witkon, K., Chen, A.I., Hadjiyane, C., Weinstein, M.I., Pellecchia, C. Neutrophil-activating peptide (interleukin-8) in colonic mucosa from patients with Crohn's disease. Scand J Gastroenterol 28(4):296-300, 1993. https://doi.org/10.3109/00365529309090244
  11. Izzo, R.S., Witkon, K., Chen, A.I., Hadjiyane, C., Weinstein, M.I., Pellecchia, C. Neutrophil-activating peptide (interleukin-8) in colonic mucosa from patients with Crohn's disease. Scand J Gastroenterol 28(4):296-300, 1993. https://doi.org/10.3109/00365529309090244
  12. Janeway, C.A. Immunobiology. 5th ed., London, Churchill-Livingstone, pp 3-80, 2001.
  13. Tizard, I.R. Immunology, An introduction inflammation. 2nd ed.,Saunders College Publishing, New York, NY, USA. pp 423-441, 1986.
  14. Willoughby, D.A. Heberden Oration, 1974. Human arthritis applied to animal models. Towards a better therapy. Ann Rheum Dis 34: 471-478, 1975. https://doi.org/10.1136/ard.34.6.471
  15. Gordon, J.R., Burd, P.R., Galli, S.J. Mast cells as a source of multifunctional cytokines, Immunol Today 11: 458-464, 1990. https://doi.org/10.1016/0167-5699(90)90176-A
  16. Mekori, Y.A., Metcalfe, D.D. Mast cells in innate immunity, Immunol Rev 173: 131-140, 2000. https://doi.org/10.1034/j.1600-065X.2000.917305.x
  17. Williams, C.M., Galli, S.J. The diverse potential effector and immunoregulatory roles of mast cells in allergic disease. J Allergy Clin Immunol 105: 847-859, 2000. https://doi.org/10.1067/mai.2000.106485
  18. Newman, S.L. Macrophages in host defense against Histoplasma capsulatum. Trends Microbiol 7(2):67-71, 1999. https://doi.org/10.1016/S0966-842X(98)01431-0
  19. Morrison, D.C., Ryan, J.L. Endotoxins and disease mechanisms. Annu Rev Med 38: 417-732, 1987. https://doi.org/10.1146/annurev.me.38.020187.002221
  20. Tracey, K.J., et al. Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia. Nature 330: 662-664, 1987. https://doi.org/10.1038/330662a0
  21. 이귀녕, 권오현. 임상병리파일, 서울, 의학문화사, p 717, 718, 721, 754, 2003.
  22. 대한내과학회. HARRISON'S 내과학(2). 서울, MIP, p 1861, 2004.
  23. Toivari, M., Maki, T., Suutarla, S., Eklund, K.K. Ethanol inhibits IgE-induced degranulation and cytokine production in cultured mouse and human mast cells. Life Sci 67: 2795-2806, 2000. https://doi.org/10.1016/S0024-3205(00)00863-8
  24. Na, H.J., Moon, P.D., Ko, S.G., Lee, H.J., Jung, H.A., Hong, S.H., Seo, Y., Oh, J.M., Lee, B.H., Choi, B.W., Kim, H.M. Sargassum hemiphyllum inhibits atopic allergic reaction via the regulation of inflammatory mediators. J Pharmacol Sci 97: 219-226, 2005. https://doi.org/10.1254/jphs.FP0040326
  25. Lee, D.H., Kim, S.H., Eun, J.S., Shin, T.Y. Mosla dianthera inhibits mast cell-mediated allergic reactions through the inhibition of histamine release and inflammatory cytokine production. Toxicol Appl Pharmacol 216: 479-484, 2006. https://doi.org/10.1016/j.taap.2006.06.007
  26. Meyer, G.K., Neetz, A., Brandes, G., Tsikas, D., Butterfield, J.H., Just, I., Gerhard, R. Clostridium difficile toxins A and B directly stimulate human mast cells. Infect Immun 75: 3868-3876, 2007. https://doi.org/10.1128/IAI.00195-07
  27. Lindemann, R.A., Economou, J.S., Rothrmel, H. Production of interleukin-1 and tumor necrosis factor by human peripheral blood monocytes activated by periodontal bacteria and extracted lipopolysaccharide. J Dent Res 67(8):1131-1135, 1988. https://doi.org/10.1177/00220345880670081401
  28. Forstermann, U., Schmidt, H.H., Pollock, J.S., Sheng, H., Mitchell, J.A., Warner, T.D., Nakane, M., Murad, F. Isoforms of nitric oxide synthase characterization and purification from different cell types. Biochem Pharmaco 42(10):1849-1857, 1991. https://doi.org/10.1016/0006-2952(91)90581-O
  29. Alderton, W.K., Cooper, C.E., Knowles, R.G. Nitric oxide synthases; structure, function and inhibition. Biochem J 357(Pt.3):593-615, 2001. https://doi.org/10.1042/bj3570593
  30. Tezuka, Y., Irikawa, S., Kaneko, T., Banskota, A.H., Nagaoka, T., Xiong, Q., Hase, K., Kadota, S. Screening of Chinese herbal drug extracts for inhibitory activity on nitric oxide production and identification of an active compound of Zanthoxylum bugeanum. J Ethnopharmacol 77: 209-217, 2001. https://doi.org/10.1016/S0378-8741(01)00300-2
  31. Kim, R.G., Shin, K.M., Chun, S.K., Ji, S.Y., Seo, S.H., Park, H.J., Choi, J.W., Lee, K.T. In vitro antiinflammatory activity of the essential oil from Ligularia fischeri var. spiciformis in murine macrophage RAW 264.7 cells. Yakhak Hoeji 46: 343-347, 2002.
  32. 서은성. 가미청상방풍탕의 항염증효과에 대한 실험적 연구, 원광대학교 대학원, 2005.
  33. 문태철, 정규찬, 손건호, 김현표, 강삼식, 장현욱. 천연물로부터 사이클로옥시게나제-2 저해제 검색. 약학회지 42: 214-219, 1998.
  34. 서영준. 발암과정에 있어서 cyclooxygenase-2의 역할 및 그 저해를 통한 화학 암 예방. 분자세포생물학뉴스 13: 8-17, 2001.