Mycobiology

The Korean Society of Mycology (한국균학회)
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Regeneration of Ectomycorrhizal Fungal Isolates Following Deep Freezer Storage

  • Obase, Keisuke (College of Forest and Environmental Sciences, Kangwon National University) ;
  • Lee, Sang-Yong (College of Forest and Environmental Sciences, Kangwon National University) ;
  • Chun, Kun-Woo (College of Forest and Environmental Sciences, Kangwon National University) ;
  • Lee, Jong-Kyu (College of Forest and Environmental Sciences, Kangwon National University)
  • Received : 2011.03.24
  • Accepted : 2011.05.13
  • Published : 2011.06.30
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Abstract

Mycelial growth and survival ratio of ectomycorrhizal fungi were determined after storage at $-70^{\circ}C$ for 1, 3, or 6 mon. Seventeen of 23 ectomycorrhizal fungi did not survive after storage for more than 6 mon, whereas Cenococcum geophilum, Lepista nuda, and some species of Rhizopogon and Suillus did survive.

Keywords

References

  1. Smith SE, Read DJ. Mycorrhizal symbiosis. 2nd ed. London: Academic Press; 1997.
  2. Rinaldi AC, Comandini O, Kuyper TW. Ectomycorrhizal fungal diversity: separating the wheat from the chaff. Fungal Divers 2008;33:1-45.
  3. Quoreshi AM. The use of mycorrhizal biotechnology in restoration of disturbed ecosystem. In: Siddiqui ZA, Akhtar MS, Futai K, editors. Mycorrhizae: sustainable agriculture and forestry. Dordrecht: Springer; 2008. p. 303-20.
  4. Smith D, Onions AH. The preservation and maintenance of living fungi. 2nd ed. Wallingford: CAB International; 1994.
  5. Ellis JJ, Roberson JA. Viability of fungus cultures preserved by lyophilization. Mycologia 1968;60:399-405. https://doi.org/10.2307/3757169
  6. Bosmans J. Ten years lyophilization of pathogenic fungi. Mycopathol Mycol Appl 1974;53:13-23. https://doi.org/10.1007/BF02127195
  7. Tan CS, Stalpers JA, van Ingen CW. Freeze-drying of fungal hyphae. Mycologia 1991;83:654-7. https://doi.org/10.2307/3760221
  8. Qiangqiang Z, Jiajun W, Li L. Storage of fungi using sterile distilled water or lyophilization: comparison after 12 years. Mycoses 1998;41:255-7. https://doi.org/10.1111/j.1439-0507.1998.tb00334.x
  9. Ryan MJ, Smith D, Jeffries P. A decision-based key to determine the most appropriate protocol for the preservation of fungi. World J Microbiol Biotechnol 2000;16:183-6. https://doi.org/10.1023/A:1008910006419
  10. Hwang SW. Long-term preservation of fungus cultures with liquid nitrogen refrigeration. Appl Microbiol 1966;14:784-8.
  11. Pertot E, Puc A, Kremser M. Lyophilization of nonsporulating strains of the fungus Claviceps. Eur J Appl Microbiol 1977;4:289-94. https://doi.org/10.1007/BF00931266
  12. Sundari SK, Adholeya A. Freeze-drying vegetative mycelium of Laccaria fraterna and its subsequent regeneration. Biotechnol Tech 1999;13:491-5. https://doi.org/10.1023/A:1008937719759
  13. Singh SK, Upadhyay RC, Yadav MC, Tiwari M. Development of a novel lyophilization protocol for preservation of mushroom mycelial cultures. Curr Sci 2004;87:568-70.
  14. Carmichael JW. Frozen storage for stock cultures of fungi. Mycologia 1956;48:378-81. https://doi.org/10.2307/3755355
  15. Kramer CL, Mix AJ. Deep freeze storage of fungus cultures. Trans Kans Acad Sci 1957;60:58-64. https://doi.org/10.2307/3627003
  16. Hwang SW. Effects of ultra-low temperatures on the viability of selected fungus strains. Mycologia 1960;52:527-9. https://doi.org/10.2307/3755974
  17. Ito T, Nakagiri A. Viability of frozen cultures of Basidiomycetes after fifteen-year storage. Microbiol Cult Coll 1996;12:67-78.
  18. Kitamoto Y, Suzuki A, Shimada S, Yamanaka K. A new method for the preservation of fungus stock cultures by deepfreezing. Mycoscience 2002;43:143-9. https://doi.org/10.1007/s102670200021
  19. Obase K, Cha JY, Lee JK, Lee SY, Lee JH, Chun KW. Ectomycorrhizal fungal communities associated with Pinus thunbergii in the eastern coastal pine forests of Korea. Mycorrhiza 2009;20:39-49. https://doi.org/10.1007/s00572-009-0262-1
  20. Marx DH. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections: I. antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology 1969;59:153-63.
  21. R Development Core Team. R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2010.

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