Mycobiology

The Korean Society of Mycology (한국균학회)
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Optimal Culture Conditions for Mycelial Growth of Lignosus rhinocerus

  • Lai, W.H. (Agro-Biotechnology Institute, Ministry of Science, Technology and Innovation, c/o Malaysian Agricultural Research and Development Institute) ;
  • Murni, M.J. Siti (Malaysian Institute of Chemical and Bioengineering Technology, Kuala Lumpur University) ;
  • Fauzi, D. (School of Biosciences & Biotechnology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia) ;
  • Mazni, O. Abas (Strategic Livestock Research Centre, Malaysian Agricultural Research and Development Institute) ;
  • Saleh, N.M. (Agro-Biotechnology Institute, Ministry of Science, Technology and Innovation, c/o Malaysian Agricultural Research and Development Institute)
  • Received : 2011.04.28
  • Accepted : 2011.05.19
  • Published : 2011.06.30
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Abstract

Lignosus rhinocerus is a macrofungus that belongs to Polyporaceae and is native to tropical regions. This highly priced mushroom has been used as folk medicine to treat diseases by indigenous people. As a preliminary study to develop a culture method for edible mushrooms, the cultural characteristics of L. rhinocerus were investigated in a range of culture media under different environmental conditions. Mycelial growth of this mushroom was compared on culture media composed of various carbon and nitrogen sources in addition to C/N ratios. The optimal conditions for mycelial growth were $30^{\circ}C$ at pH 6 and 7. Rapid mycelial growth of L. rhinocerus was observed on glucose-peptone and yeast extract peptone dextrose media. Carbon and nitrogen sources promoting mycelial growth of L. rhinocerus were glucose and potassium nitrate, respectively. The optimum C/N ratio was approximately 10 : 1 using 2% glucose supplemented as a carbon source in the basal media.

Keywords

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