대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

배양한 흰쥐 대뇌세포의 저산소증 모델에서 황련(黃連)이 유전자 표현에 미치는 영향

Effects of Gene expression by Coptidis chinesis FRANCH. in a Hypoxic Model of Cultured Rat Cortical Cells

  • 황주원 (동국대학교 한의과대학 내과학교실) ;
  • 김경훈 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실) ;
  • 문일수 (동국대학교 의과대학 해부학교실)
  • Hwang, Joo-Won (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Kim, Kyung-Hun (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Shin, Gil-Cho (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Guk University) ;
  • Moon, Il-Soo (Dept. of Anatomy, College of Medicine, Dong-Guk University)
  • 발행 : 2011.06.30
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초록

Objectives : The purpose of this investigation was to evaluate the effects of Coptidis chinesis FRANCH. on the alteration of gene expression in a hypoxic model using cultured rat cortical cells. Methods : E18 rat cortical cells were grown in neurobasal medium containing B27 supplement. On 12 DIV, water extract from Coptidis chinesis FRANCH. was added ($20{\mu}g/ml$) to the culture media 4 hrs. On 14 DIV, cells were given hypoxic insult (2% $O_2$/5% $CO_2$, $37^{\circ}C$, 3 hrs), returned to normoxia and cultured for another 24 hrs. Total RNA was extracted from Coptidis chinesis FRANCH. treated and untreated cultures and alterations in the gene expression were analysed by microarray using rat 5K-TwinChips. Results : Effects on some of the genes whose functions were implicated in neural viability were as follows: the expression of apoptosis-related genes such as Clu (Global M = 1.3), of presynaptic inhibition's genes such as Penk-rs (Global M = 1.97), and of innate immuniti's such as Crp (Global M = 1.95), Defensin (Global M = 2.14), and Dnase1l3 (Global M = 1.57) increased. The expression of neurotrophic genes such as S100b (Global M = 1.42), and $NF{\kappa}B$ (Global M = 2.04) increased. Conclusions : Analysing the genes expressed on microarray, shows Coptidis chinesis FRANCH.protects cells by increasing viability and neural nutrition.

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