The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Antioxidant Activity of Sesami Semen Nigrum on Leydig TM3 cells

흑지마(黑芝麻)가 Leydig cell의 항산화에 미치는 영향

  • Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Chung, Kyu-Jin (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Chang, Won-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 정규진 (경희대학교 한의과대학 처방제형학교실) ;
  • 장원규 (경희대학교 한의과대학 처방제형학교실) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Received : 2011.02.18
  • Accepted : 2011.03.10
  • Published : 2011.03.30
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Abstract

Objectives : The purpose of this study was to estimate the antioxidant activity of Sesami Semen Nigrum extract (SSN) on mouse Leydig cells, TM3. Methods : Cell viability assays were performed. The protective effects of SSN against hydrogen peroxide-induced oxidative stress in Leydig cells were examined by measuring cell viability. Lipid peroxidation levels and antioxidant enzyme concentrations such as SOD and catalase were measured. Results : Cell viability of Leydig cells increased with SSN concentration. Cell viability of Leydig cells was 136.66% when SSN concentration was $50{\mu}g/ml$. Cell viability of the hydrogen peroxide group was statistically decreased (p<0.01) compared with the control group. Antioxidant effect of SSN was measured and the protective effect of SSN concentration were 5, 10, $50{\mu}g/ml$. LPO were decreased significantly at 5, $50{\mu}g/ml$ of SSN concentrations. SOD activity was increased at 1, 10, $50{\mu}g/ml$ of SSN concentrations. Catalase activity was significantly increased at 123.7, 133.3 and 131.9 units/mg protein when SSN concentrations were 5, 10 and $50{\mu}g/ml$, respectively. Conclusions : In conclusion, Sesami Semen Nigrum extract has antioxidant activities in Leydig cells against oxidative stress.

Keywords

References

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