The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Discrimination of Chinese Glycyrrhiza uralensis and Uzbek Glycyrrhiza glabra Using Taste Sensor

미각센서를 이용한 중국산 감초와 우즈베키스탄산 광과감초의 감별

  • Choi, Go-Ya (Creative Research Team, Korea Institute of Oriental Medicine) ;
  • Kim, Young-Hwa (Creative Research Team, Korea Institute of Oriental Medicine) ;
  • Chae, Sung-Wook (Creative Research Team, Korea Institute of Oriental Medicine) ;
  • Lee, Hye-Won (Creative Research Team, Korea Institute of Oriental Medicine) ;
  • Ko, Byoung-Seob (Creative Research Team, Korea Institute of Oriental Medicine) ;
  • Lee, Mi-Young (Creative Research Team, Korea Institute of Oriental Medicine)
  • 최고야 (한국한의학연구원 창의연구실) ;
  • 김영화 (한국한의학연구원 창의연구실) ;
  • 채성욱 (한국한의학연구원 창의연구실) ;
  • 이혜원 (한국한의학연구원 창의연구실) ;
  • 고병섭 (한국한의학연구원 창의연구실) ;
  • 이미영 (한국한의학연구원 창의연구실)
  • Received : 2011.02.14
  • Accepted : 2011.03.10
  • Published : 2011.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Genetic analysis and taste pattern were performed to identify species between Glycyrrhiza uralensis and G. glabra which are officially listed in Korean Pharmacopoeia IX as origin of Gamcho(g$\={a}$nc$\v{a}$o, licorice root, Glycyrrhizae Radix et Rhizoma). Methods : Genetic analysis showed that identification between two species was done by comparing base sequence of ITS(intergenic transcribed spacer) and trnH-psbA regions from eleven Gamchoes sold in market. There was different taste pattern using by taste sensor in Glycyrrhiza uralensis and G. glabra. Results : Genetic analysis showed that six Gamchoes from China were identified as Glycyrrhiza uralensis and five Gamchoes from Uzbekistan were G. glabra. From the results of taste pattern, sourness and astringency of Glycyrrhiza uralensis from China were significantly higher than G. glabra from Uzbekistan, and aftertaste of astringency, aftertaste of umami, and saltiness of Glycyrrhiza uralensis were signicantly low as compared to G. glabra. There is no significant difference between two species in terms of bitterness, aftertaste of bitterness, and umami. Conclusions : Taken together, Glycyrrhiza uralensis from China and G. glabra from Uzbekistan were identified by taste sensor, and this technic could be applied to establishment of taste pattern marker for identification of different species located in various regions.

Keywords

References

  1. 식품의약품안전청. 대한약전 제9개정. 2007 : 903.
  2. 식품의약품안전청. 원색 한약재감별도감. 2009 : 17-9, 530-1.
  3. 楊光興, 劉力, 劉偉新. 新疆5種甘草紫外光譜, 薄層層析及其薄層掃描的鑑別硏究. 新疆中醫藥. 1997 ; 15(1) : 33-4.
  4. 田進國, 任健, 婁紅祥, 高彦慧, 傅密寧, 傅克治. 四種甘草的紅外光譜鑑別. 中藥材. 1997 ; 20(4) : 175-6.
  5. 김재욱, 길기정, 이영종. 감초 분말의 현미조직에 관한 연구. 대한본초학회지. 2003 ; 18(4) : 101-7.
  6. 이영종, 조진희. 감초의 형태 감별에 관한 연구. 대한본초학회지. 2004 ; 19(3) : 47-52.
  7. 劉寶玲, 王英華, 劉曉芳. 栽培甘草與野生甘草的形態組織學硏究. 中國藥事. 2005 ; 19(7) : 431-2.
  8. Kondo K, Shiba M, Yamaji H, Morota T, Zhengmin C, Huixia P, Shoyama Y. Species identification of licorice using nrDNA and cpDNA genetic markers. Biol Pharm Bull. 2007 ; 30(8) : 1497-502. https://doi.org/10.1248/bpb.30.1497
  9. Anjiki N, Kawahara N, Goda Y. Evaluation of the Taste of Kampo Formulae by Taste-Sensing System (1). Natural Medicines. 2005 ; 59(4) : 164-70.
  10. Hayashi N, Chen R, Ikezaki H, Yamaguchi S, Maruyama D, Yamaguchi Y, Ujihara T, Kohata K. Techniques for Universal Evaluation of Astringency of Green Tea Infusion by the Use of a Taste Sensor System. Biosci Biotechnol Biochem. 2006 ; 70(3) : 626-31. https://doi.org/10.1271/bbb.70.626
  11. Kawahara N, Anjiki N, Hosoe J, Kim IH, Ikezaki H, Mikage M, Goda Y. Studies on Relationship between Taste and Content of Sulfur Dioxide in Crude Drugs Obtained from the Japanese Market. Pharm Regul Sci. 2009 ; 40(3) : 129-35.
  12. Kobayashi Y, Habara M, Ikezazki H, Chen R, Naito Y, Toko K. Advanced Taste Sensors Based on Artificial Lipids with Global Selectivity to Basic Taste Qualities and High Correlation to Sensory Scores. Sensors. 2010 ; 10 : 3411-43. https://doi.org/10.3390/s100403411
  13. White TJ, Bruns T, Lee S, Taylor T. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In : Innis MA, Gelfand DH, Shinsky JJ, White TJ, eds. In PCR Protocol s : A guide to methods and applications. San Diego : Academic Press. 1990 : 315-22.
  14. Demesure B, Sodzi N, Petit RJ. A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol. 1995 ; 4(1) : 129-31. https://doi.org/10.1111/j.1365-294X.1995.tb00201.x
  15. 주영승. 운곡본초학 총론. 서울 : 서림재. 2002 : 377.
  16. 國家藥典委員會. 中華人民共和國藥典. 北京 : 中國醫藥科技出版社. 2010 : 80.
  17. 厚生労働省. 第十五改正日本薬局方. 2006 : 1197.
  18. 식품의약품안전청. 한약재 관능검사지침 I. 2006 : 17.
  19. Wu ZY, Raven PH, Hong DY, eds. Flora of China. Vol. 10 (Fabaceae). Beijing : Science Press, St. Louis : Missouri Botanical Garden Press. 2010 : 509-10.