Journal of Plant Biotechnology

  • 제38권2호
  • /
  • Pages.169-175
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  • 2011
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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애기장대에서 두 액포막 칼슘펌프 돌연변이에 의하여 유도되는 세포사멸 표현형의 액포수식효소(VPE) 돌연변이에 의한 억제

The vacuolar processing enzyme (VPE) mutation suppresses an HR-like cell death induced by the double knockout mutant of vacuolar Ca2+-ATPases in Arabidopsis

  • 박형철 (경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작 연구소) ;
  • 이상민 (경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작 연구소) ;
  • 김호수 (경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작 연구소) ;
  • 정우식 (경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작 연구소)
  • Park, Hyeong-Cheol (Division of Applied Life Science (BK21 program), and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Sang-Min (Division of Applied Life Science (BK21 program), and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Kim, Ho-Soo (Division of Applied Life Science (BK21 program), and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Chung, Woo-Sik (Division of Applied Life Science (BK21 program), and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
  • 투고 : 2011.04.15
  • 심사 : 2011.04.29
  • 발행 : 2011.06.30
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초록

칼슘은 동물과 마찬가지로 식물에서도 다양한 신호를 전달하는 2차 매개체로 잘 알려져 있다. 특히, 세포사멸 현상을 유도하는 신호전달에 칼슘의 관여는 잘 보고되어 있다. 최근 발표된 논문에서 액포막에 존재하는 $Ca^{2+}$-ATPases(ACA4와 ACA11)가 이중으로 돌연변이된 식물체의 잎에서 세포사멸 표현형을 관찰했으며, 이러한 세포사멸 현상이 salicylic acid (SA)에 의존적이라고 보고했다. 또한 앞선 연구들에서 vacuolar processing enzymes (VPEs)이 생물학적 또는 비생물학적 스트레스에 의해서 유발되는 SA에 의해서 활성화 된다고 보고하였다. 본 연구에서는 액포막 $Ca^{2+}$-ATPases (ACA4와 ACA11)가 이중으로 돌연변이 된 식물체에서 VPEs의 유전자 발현이 상당히 증가되어 있고 효소 활성도 증가됨을 확인했다. 이 결과를 바탕으로, aca4/aca11/avpe와 aca4/aca11/${\gamma}$vpe 그리고, aca4/aca11/avpe/${\gamma}$vpe의 삼중과 사중 돌연변이체를 구축했다. 이들과 aca4/aca11의 이중 돌연변이체의 세포사멸 표현형을 비교 관찰한 결과, 삼중과 사중 돌연변이에서 세포사멸 현상이 일정기간 억제되는 것을 관찰했다. 또한, 삼중과 사중의 돌연변이체에서 VPEs의 효소 활성이 많이 감소되는 현상으로 나타났다. 결론적으로, $Ca^{2+}$-ATPases (ACA4와 ACA11)가 이중으로 돌연변이된 식물체에서는 SA가 유발되며, 이러한 SA에 의해서 VPEs의 단백질이 활성화되어 세포사멸 현상이 발생할 것으로 사료된다.

Calcium ($Ca^{2+}$) signals have been implicated in regulating plant development and responses to the environmental stresses including a programmed cell death pathway. In animals and plants, cytosolic $Ca^{2+}$ signals have been involved in the activation of programmed cell death (PCD). Recently, we reported that disruption of Arabidopsis vacuolar $\b{A}$utoinhibited $\underline{C}a^{2+}$-$\b{A}$TPases (ACAs), ACA4 and ACA11, resulted in the activation of a salicylic acid-dependent programmed cell death pathway. Although extensive studies have revealed various components of a PCD in plants, executors to directly induce PCD are well unknown. Here, we provide that the vacuolar processing enzymes (VPEs) are involved in a PCD induced by the double knockout mutant of vacuolar $Ca^{2+}$-ATPases in Arabidopsis. The gene expression of VPE was rapidly up-regulated and the enzyme activity of VPE was increased in the double mutant plants. We also generated aca4/aca11/avpe, aca4/aca11/${\gamma}$vpe and aca4/aca11/avpe/${\gamma}$vpe mutant plants. Although cell death phenotype of the double mutant plants was not completely disappeared in the triple and quadruple mutant plants, the triple and quadruple mutant plants showed to significantly delay cell death phenotype of the double mutant plants. These results suggest that the VPE is involved in the HR-like cell death in the double mutant of vacuolar $Ca^{2+}$-ATPases in Arabidopsis.

키워드

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