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호두의 화장품 기능성

Functional Properties of Walnut in Cosmetics

  • 김미경 (영동대학교 고령친화산업기업지원센터) ;
  • 김진성 (경북대학교 식품과학부) ;
  • 조분성 (경북대학교 식품과학부) ;
  • 김정환 (경북대학교 나노소재공학부) ;
  • 이인철 (영동대학교 고령친화산업기업지원센터) ;
  • 이명섭 (영동대학교 화장품과학과) ;
  • 조영제 (경북대학교 식품공학부, 식품생물산업연구소)
  • Kim, Mee-Kyung (Center for Senior Industry, Youngdong University) ;
  • Kim, Jin-Sung (School of Food Science, Kyungpook National University) ;
  • Jo, Bun-Sung (School of Food Science, Kyungpook National University) ;
  • Kim, Jeung-Hoan (School of Nano Material Engineering. Kyungpook National University) ;
  • Lee, In-Cheol (Center for Senior Industry, Youngdong University) ;
  • Lee, Myung-Sup (Department of Cosmetic Science and Technology, Youngdong University) ;
  • Cho, Young-Je (School of Food Science & Biotechnology, Food &Bio-Industry Research Institute, Kyungpook National University)
  • 투고 : 2011.04.04
  • 심사 : 2011.05.18
  • 발행 : 2011.06.30

초록

호두과육으로부터 용매별로 추출한 추출액중의 phenolic compound 함량은 열수, 에탄올, 메탄올, 아세톤 추출물 순서로 24.3, 34.4, 32.5, 15.1 mg/g의 함량을 나타내어 에탄올 추출물의 phenolic compound 함량이 가장 높았으며, 인체에 무해한 추출 용매인 열수 및 에탄올 농도별 추출물의 phenolic compound 비교한 결과 60% 에탄올 추출물에서 34.7 mg/g과 열수 추출물에서 24.6 mg/g 함량의 phenolic compound를 나타내었다. 호두추출물의 항산화 활성 중 전자공여능의 경우 열수추출물에서 78.1%, 60% 에탄올추출물에서 80.6%의 활성을 나타내었으며, ABTS radical cation decolorization 측정에서는 열수추출물과 60% 에탄올추출물 각각 98.1%와 98.3%의 높은 항산화 활성을 나타내었다. Antioxidant protection factor (PF)의 경우는 열수추출물과 60% 에탄올추출물에서 각각 $1.1{\pm}0.2$ PF와 $1.1{\pm}0.4$ PF로 높지는 않았으며, TBARs inhibitory activity 측정에서는 열수추출물과 60% 에탄올추출물에서 각각 60%와 75%로 비교적 높은 항산화력을 가졌음이 확인되었다. 호두추출물의 항염증효과를 살펴본 결과 100 ${\mu}g/ml$의 농도에서 50%의 NO발현이 억제되었고, western blot에 의한 iNOS 및 COX-2의 억제효과는 대조군에 비해 100 ${\mu}g/ml$의 농도에서 10% 정도의 낮은 저해 효과를 나타내었다. Tyrosinase의 저해 활성의 경우 60% 에탄올추출물에서 43%의 저해율을 보였으며, 수렴효과의 경우 60% 에탄올추출물에서 55%의 억제효과를 나타내어, 호두추출물은 미백 및 주름개선용 화장품소재로서 개발이 가능하다고 판단되었다. 본 연구는 고령친화산업 기업지원센터의 연구비 지원에 의하여 수행되었으며, 이에 감사드립니다.

The phenolic compounds of walnut extracts by various solvents were shown to be 24.3 mg/g in hot water, 34.4 mg/g in ethanol, 32.5 mg/g in methanol and 15.1 mg/g in acetone. In a comparison of phenolic compounds from hot water and different concentrations of ethanol, which are harmless, 60% ethanol extract and hot water extract were 34.7 mg/g, 24.6 mg/g. The electron donating ability (EDA) of walnut extracts in hot water and 60% ethanol were 78.1% and 80.6%. According to ABTS radical cation decolorization for antioxidant activity, hot water and 60% ethanol extract showed high antioxidant activities of 98.1% and 98.3%. Antioxidant protection factor (PF) were $1.1{\pm}0.2$ PF and $1.1{\pm}0.4$ PF in hot water and 60% ethanol extract. In TBARs inhibitory activity, each extract showed high antioxidant activities at 60% and 75%. Anti-inflammation effects of walnut extract were tested, and inhibition of NO was 50% in 100 ${\mu}g/ml$ phenolics. Inhibitory activity against iNOS and COX-2 were shown, through Western blot, to be 10% in 100 ${\mu}g/ml$ phenolics. Tyrosine inhibitory activity of 60% ethanol extract was 43%, and astringent effect of 60% ethanol extract was 55%. These results suggest that walnut extracts are suitable for functional cosmetics requiring skin-whitening and anti-wrinkle activity.

키워드

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