Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Study on Norovirus Genotypes in Busan, Korea

부산지역에서 분리된 norovirus 유전자형 연구

  • Kim, Nam-Ho (Busan Metropolitan City Institute of Health and Environment) ;
  • Park, Eun-Hee (Busan Metropolitan City Institute of Health and Environment) ;
  • Park, Yon-Koung (Busan Metropolitan City Institute of Health and Environment) ;
  • Min, Sang-Kee (Busan Metropolitan City Institute of Health and Environment) ;
  • Jin, Seong-Hyeon (Busan Metropolitan City Institute of Health and Environment) ;
  • Park, So-Hyun (Busan Metropolitan City Institute of Health and Environment)
  • 김남호 (부산광역시 보건환경연구원 역학조사과) ;
  • 박은희 (부산광역시 보건환경연구원 역학조사과) ;
  • 박연경 (부산광역시 보건환경연구원 역학조사과) ;
  • 민상기 (부산광역시 보건환경연구원 역학조사과) ;
  • 진성현 (부산광역시 보건환경연구원 역학조사과) ;
  • 박소현 (부산광역시 보건환경연구원 역학조사과)
  • Received : 2011.03.31
  • Accepted : 2011.05.16
  • Published : 2011.06.30
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Abstract

Norovirus (NoV) causes major acute non-bacterial gastroenteritis in humans. NoV genus is a member of the family Caliciviridae, which is transmitted by contaminated food and water or from human to human. Many genotypes of genogroups I and II have been reported because of their high genetic diversity. To obtain molecular epidemiological information on gastroenteritis sporadic cases in Busan, Korea, we analyzed the nucleotide sequences of NoV strains detected during 2008~2010. We performed one step RT-PCR amplifying the open reading frame (ORF) 2 (capsid region) followed by semi-nested PCR. Fecal samples were collected from 4,071 acute gastroenteritis, and genotypes of the 421 positive samples were determined by sequence analysis. Based on partial sequence of capsid region, 7 NoV were categorized into genogroup I and 13 into genogroup II. Prevalent genotypes among gastroenteritis patients within Busan were GII.4, GI.6, GII.5 in 2008~2010. The results of this study will contribute to the currently available epidemiological data and improve public health and hygiene via development of diagnostic methods and sustainable surveillance.

2008년부터 2010년까지 최근 3년 동안 부산지역에서 산발적으로 발생한 급성 위장관염 환자를 대상으로 유전자를 검사한 결과 4,101건 중 426건(10.4%)에서 노로바이러스를 확인하였다. 연도별 검출현황은 2008년에 14.7%(222/1,506), 2009년에 6.9%(95/1,384), 2010년에 9.0%(109/1,211)로 나타났다. 월별 분석 결과는 2008년에는 3월에 35.7%(50/140)로 가장 높은 검출율을 보였고, 2009년 역시 3월에 21.9%(23/105)로 높게 나타났으며, 2010년에는 1월에 23.8%(29/122)로 높은 검출율을 보여 겨울절기에 노로바이러스가 유행하는 것을 알 수 있었다. 반면 매해 7-8월 여름절기에는 노로바이러스가 거의 분리되지 않았다. 연령별 로는 1세 영아군과 13-19세 중등학생군에서 각각 20.9%로 가장 높은 검출율을 나타내었으며, 2-6세 소아군에서 17.5%, 20-29세군에서 13.4%, 7-12세 초등학생군에서 12.7%, 30-39세군에서 9.1%, 0세 신생아군에서 8.7%, 50-59세군에서 7.2%, 60-69세군과 70세이상군에서 각각 6.7%, 40-49세 4.5%로 확인되었다. 노로바이러스 양성 검체 340건에서 유전자형을 분석한 결과 GI군 7종류, GII군 13종류로 총 20종류가 검출되어 다양한 유전자형의 노로바이러스들이 유행함을 알 수 있었다. 연구 결과 부산지역에서는 GI군이 21.8%(76/348), GII군이 78.2%(272/348)로 GII군이 우세하여 유행하였고, 유전자형 총 20종 중 GII.4형이 49.1%로 가장 많이 검출되었다.

Keywords

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