Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Role of Dual Flagella in the Pathogenesis of Vibrio parahaemolyticus

  • Lee, Hwa-Gyu (Department of Food Science and Biotechnology, College of Ocean Science and Technology, Kunsan National University) ;
  • Jeong, Byung-Gon (Department of Environmental Engineering, College of Engineering, Kunsan National University) ;
  • Park, Kwon-Sam (Department of Food Science and Biotechnology, College of Ocean Science and Technology, Kunsan National University)
  • Received : 2011.03.01
  • Accepted : 2011.05.16
  • Published : 2011.06.30
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Abstract

Vibrio parahaemolyticus possesses two flagella systems: polar and lateral flagella for swimming in liquid and swarming on solid surfaces or in viscous environments. To elucidate the pathogenic role of these dual flagella systems, we constructed single- and double-deletion mutants of the lafA and flhAB flagellum genes and investigated their biofilm formation, cell adhesion, and colonization of the small intestine of suckling mice. The double-mutant strain was more impaired in biofilm formation than either of the single-mutant strains. In addition, the lafA, flhAB, and double-mutant strains showed 40%, 45%, and 60%, respectively, lower adherence to HeLa cells than the wild-type strain. Moreover, the lafA, flhAB, and double-mutant strains exhibited 49%, 5.6 and 6.7 times, respectively, lower colonization in a competition assay than the wild-type strain. These findings indicated that polar flagella were more important than lateral flagella for the pathogenesis of V. parahaemolyticus.

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