Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Analysis of anatoxin-a in aqueous and cyanobacterial samples from korean lakes by liquid chromatography with fluorescence detection

액체크로마토그래피-형광검출법에 의한 호소시료의 아나톡신-a 분석

  • Lee, In-Jung (Nakdong River Environment Research Center, National Institute of Envirnonmental Research) ;
  • Lee, Chul-Gu (Nakdong River Environment Research Center, National Institute of Envirnonmental Research) ;
  • Heo, Seong-Nam (Nakdong River Environment Research Center, National Institute of Envirnonmental Research) ;
  • Lee, Jae-Gwan (Nakdong River Environment Research Center, National Institute of Envirnonmental Research)
  • 이인정 (국립환경과학원 낙동강물환경연구소) ;
  • 이철구 (국립환경과학원 낙동강물환경연구소) ;
  • 허성남 (국립환경과학원 낙동강물환경연구소) ;
  • 이재관 (국립환경과학원 낙동강물환경연구소)
  • Received : 2011.05.04
  • Accepted : 2011.05.18
  • Published : 2011.06.25
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Abstract

Anatoxin-a is a cyanobacterial neurotoxin with a high toxicity produced by Anabaena, Aphanizomenon and Oscillatoria. Water bloom, formed by Anabaena has been occurring frequently in Lake Yeongchun. It is need to develop a sensitive method for determination of anatoxin-a to control potential hazard in raw water resources. In this study, we developed a highly sensitive analytical method of anatoxin-a using solid phase extraction (SPE) and high performance liquid chromatography (HPLC) with fluorescence detection. Anatoxin-a was converted into a highly fluorescent derivative using 4-fuoro-7-nitro-2,1,3-benzoxadiazole (NBF-F). The method was evaluated in terms of linearity of calibration curve, recovery and repeatability, and the adequate values were obtained. The method detection limit was $0.034\;{\mu}g/g$ and $0.022\;{\mu}g/L$ for algal and water samples, respectively. The concentrations of anatoxin-a were measured in algal and water samples from Lake Andong, Yeongchun and Daechung and ranged from $0.135\;{\mu}g/g$ to $10.979\;{\mu}g/g$ in algal samples and not detected in water samples.

아나톡신-a는 Anabaena, Aphanizomenon, Oscillatoria 등의 남조류에서 생성되는 신경독소로 강한 독성을 나타낸다. 국내에서는 영천호 등의 호소에서 최근 Anabaena에 의한 수화현상이 늘어나고 안전한 상수원수의 확보를 위하여 아나톡신-a의 미량분석이 필요한 실정이다. 본 연구에서는 아나톡신-a를 고상추출한 뒤 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F)로 형광 유도체화시켜 고성능액체크로마토그래프-형광검출기(HPLC-FLD)로 분석하였다. 시험방법 validation 결과 직선성, 회수율, 재현성에서 모두 좋은 값을 나타내었으며, 방법검출한계(MDL)은 조체 시료의 경우 $0.034\;{\mu}g/g$, 물 시료의 경우 $0.022\;{\mu}g/L$로 비교적 좋은 감도를 얻을 수 있었다. 국내 안동호, 영천호 및 대청호에서 채취한 조체와 물 시료에서 아나톡신-a의 농도를 분석한 결과 조체 시료에서는 $0.135\sim10.979\;{\mu}g/g$의 농도로 검출되었으며, 물 시료에서는 검출되지 않았다.

Keywords

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