Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Enhancement of centelloside production from cultured plants of Centella asiatica by combination of thidiazuron and methyl jasmonate

  • Yoo, Nam-Hee (Industrial Cooperation Foundation, Chonbuk National University) ;
  • Kim, Ok-Tae (Planning and Coordination Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Jung-Bong (Functional Food and Nutrition Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Sun-Hee (Department of Biology, Chonnam National University) ;
  • Kim, Young-Chang (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Bang, Kyong-Hwan (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hyun, Dong-Yun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cha, Seon-Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Min-Young (Jeonnam Agricultural Research and Extension Service) ;
  • Hwang, Baik (Department of Biology, Chonnam National University)
  • Received : 2010.08.09
  • Accepted : 2011.02.28
  • Published : 2011.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of made-cassoside and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation.

Keywords

References

  1. Bae KH, Choi YE, Shin CG, Kim YY, Kim YS (2006) Enhanced ginsenoside productivity by combination of ethephon and methyl jasmonate in ginseng (Panax ginseng C.A. Meyer) adventitious root cultures. Biotechnol Lett 28:1163-1166 https://doi.org/10.1007/s10529-006-9071-1
  2. Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci USA 89:2389-2393 https://doi.org/10.1073/pnas.89.6.2389
  3. Hartmann MA, Benveniste P (1987) Plant membrane sterols: isolation, identification and biosynthesis. Methods Enzymol 148:632-650
  4. He JX, Fujioka S, Li TC, Kang SG, Seto H, Takatsuto S, Yoshida S, Jang JC (2003) Sterols regulate development and gene expression in Arabidopsis. Plant Physiol 131:1258-1269 https://doi.org/10.1104/pp.014605
  5. Kim OT, Kim MY, Hong MH, Ahn JC, Hwang B (2004a) Stimulation of asiaticoside production from Centella asiatica whole plant cultures by elicitors. Plant Cell Rep 23:339-344 https://doi.org/10.1007/s00299-004-0826-7
  6. Kim OT, Kim MY, Huh SM, Ahn JC, Seong NS, Hwang B (2004b) Effect of growth regulators on asiaticoside production in whole plant cultures of Centella asiatica (L.) Urban. J Plant Biol 47:361-365
  7. Kim OT, Kim MY, Ahn JC, Hwang B (2005a) Up-regulation of asiaticoside biosynthesis by methyl jasmonate and thidiazuron in Centella asiatica L. Urban. Korean J Med Crop Sci 13:233-236
  8. Kim OT, Kim MY, Hwang SJ, Ahn JC, Hwang B (2005b) Cloning and molecular analysis of cDNA encoding cycloartenol synthase from Centella asiatica (L.) Urban. Biotechnol Bioprocess Eng 10:16-22 https://doi.org/10.1007/BF02931177
  9. Kim YS, Yeung EC, Hahn EJ, Paek KY (2007) Combined effects of phytohormone, indole-3-butyric acid, and methyl jasmonate on root growth and ginsenoside production in adventitious root cultures of Panax ginseng C.A. Meyer. Biotechnol Lett 29:1789-1792 https://doi.org/10.1007/s10529-007-9442-2
  10. Lee JE, Kim JY, Park J, Hong S, Kim J, Hyun C, Kim SY, Park DH (2006) Asiaticoside induces human collagen I synthesis through TGF$\beta$ receptor I kinase (T$\beta$RI kinase)-independent smad signaling. Planta Med 72:324-328 https://doi.org/10.1055/s-2005-916227
  11. Liu XN, Zhang XQ, Sun JS (2007) Effects of cytokinins and elicitors on the production of hypericins and hyperforin metabolites in Hypericum sampsonii and Hypericum perforatum. Plant Growth Regul 53:207-214 https://doi.org/10.1007/s10725-007-9220-0
  12. Lui M, Dai Y, Li Y, Huang F, Gong Z, Meng Q (2008) Madecassoside isolated from Centella asiatica herbs facilitates burn wound healing in mice. Planta Med 74:809-815 https://doi.org/10.1055/s-2008-1074533
  13. Mangas S, Bonfill M, Osuna L, Moyano E, Tortoriello J, Cusido RM, Pinol MT, Palazon J (2006) The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants. Phytochemistry 67:2041-2049 https://doi.org/10.1016/j.phytochem.2006.06.025
  14. Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Cytokinin activity of N-phenyl-$\aleph$-1,2,3-thiadiazol-5- ylurea (thidiazuron). Phytochemistry 21:1509-1511 https://doi.org/10.1016/S0031-9422(82)85007-3
  15. Nkembo KM, Lee JB, Hayashi T (2005) Selective enhancement of scopadulcic acid B production in the cultured tissue of Scoparia dulcis by methyl jasmonate. Chem Pharm Bull 53:780-782 https://doi.org/10.1248/cpb.53.780
  16. Saniewski M, Nowacki J, Czapski J (1987) The effect of methyl jasmonate on ethylene production and ethylene-forming enzyme activity in tomatoes. J Plant Physiol 129:199-203
  17. Weidhase RA, Lehmann J, Kramell H, Sembder G, Parthier B (1987) Degradation of ribulose-1, 5-biphosphate carboxylase and chlorophyll in senescing barley leaf segments triggered by jasmonic acid methyl ester and counteraction by cytokinin. Physiol Plant 69:161-166 https://doi.org/10.1111/j.1399-3054.1987.tb01961.x

Cited by

  1. Biotechnological production of centellosides in cell cultures of Centella asiatica (L) Urban vol.14, pp.6, 2011, https://doi.org/10.1002/elsc.201300164
  2. Chemical elicitors versus secondary metabolite production in vitro using plant cell, tissue and organ cultures: recent trends and a sky eye view appraisal vol.126, pp.1, 2011, https://doi.org/10.1007/s11240-016-0985-6
  3. Effects of methyl jasmonate on the growth and triterpenoid production of diploid and tetraploid Centella asiatica (L.) Urb. hairy root cultures vol.9, pp.1, 2011, https://doi.org/10.1038/s41598-019-54460-z