Plant Biotechnology Reports

  • 제5권3호
  • /
  • Pages.245-254
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  • 2011
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  • 1863-5466(pISSN)
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  • 1863-5474(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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An easy and efficient protocol in the production of pflp transgenic banana against Fusarium wilt

  • Yip, Mei-Kuen (Institute of Plant and Microbial Biology, Academia Sinica) ;
  • Lee, Sin-Wan (Tissue Culture Section, Taiwan Banana Research Institute) ;
  • Su, Kuei-Ching (Institute of Plant and Microbial Biology, Academia Sinica) ;
  • Lin, Yi-Hsien (Institute of Plant and Microbial Biology, Academia Sinica) ;
  • Chen, Tai-Yang (Institute of Plant and Microbial Biology, Academia Sinica) ;
  • Feng, Teng-Yung (Institute of Plant and Microbial Biology, Academia Sinica)
  • 투고 : 2011.03.02
  • 심사 : 2011.04.12
  • 발행 : 2011.07.31
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초록

This study describes an efficient protocol for Agrobacterium tumefaciens-mediated transformation of two subgroups of genotype AAA bananas (Musa acuminata cv. Pei Chiao and Musa acuminata cv. Gros Michel). Instead of using suspension cells, cauliflower-like bud clumps, also known as multiple bud clumps (MBC), were induced from sucker buds on MS medium containing $N^6$-Benzylaminopurine (BA), Thidiazuron (TDZ), and Paclobutrazol (PP333). Bud slices were co-cultivated with A. tumefaciens C58C1 or EHA105 that carry a plasmid containing Arabidopsis root-type ferredoxin gene (Atfd3) and a plant ferredoxin-like protein (pflp) gene, respectively. These two strains showed differences in transformation efficiency. The EHA105 strain was more sensitive in Pei Chiao, 51.3% bud slices were pflp-transformed, and 12.6% slices were Atfd3-transformed. Gros Michel was susceptible to C58C1 and the transformation efficiency is 4.4% for pflp and 13.1% for Atfd3. Additionally, gene integration of the putative pflp was confirmed by Southern blot. Resulting from the pathogen inoculation assay, we found that the pflp transgenic banana exhibited resistance to Fusarium oxysporum f. sp. cubense tropical race 4. This protocol is highly advantageous to banana cultivars that have difficulties in setting up suspension cultures for the purpose of quality improvement through genetic transformation. In addition, this protocol would save at least 6 months in obtaining explants for transformation and reduce labor for weekly subculture in embryogenic cell suspension culture systems.

키워드

참고문헌

  1. Arinaitwe GS, Remy H, Strosse R, Sagi L (2004) Agrobacterium and particle bombardment-mediated transformation of wide range of banana cultivars. In: Mohan Jain S, Swennen R (eds) Banana improvement: cellular, molecular biology and induced mutations, ch. 29. Science Publishers, Enfield
  2. Becker DK, Dugdale B, Smith MK, Harding RM, Dale JL (2000) Genetic transformation of Cavendish banana (Musa spp. AAA group) cv. 'Grand Nain' via microprojectile bombardment. Plant Cell Rep 19:229-234 https://doi.org/10.1007/s002990050004
  3. Belarmino MM, Mii M (2000) Agrobacterium-mediated genetic transformation of a phalaenopsis orchid. Plant Cell Rep 19:435-442 https://doi.org/10.1007/s002990050752
  4. Dayakar BV, Lin HJ, Chen CH, Ger MJ, Lee BH, Pai CH, Chow D, Huang HE, Hwang SY, Chung MC, Feng TY (2003) Ferredoxin from sweet pepper (Capsicum annuum L.) intensifying harpinpss- mediated hypersensitive response shows an enhanced production of active oxygen species (AOS). Plant Mol Biol 51:913-924 https://doi.org/10.1023/A:1023061303755
  5. De Garcıa E, Villarroel C (2007) Transgenic plantain (cv. Harton) plants resistant to herbicide basta obtained by electroporation. In:Litz RE, Scorza R (eds) Proceedings of the international symposium on biotechnology of temperate fruit crops and tropical species ISHS Acta Hort 738. ISHS, Belgium, pp 509-514
  6. Deblaere R, Bytebier B, De Greve H, Deboeck F, Schell J, Van Montagu M, Leemans J (1985) Efficient octopine Ti plasmidderived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Res 13:4777-4788 https://doi.org/10.1093/nar/13.13.4777
  7. FAO (2007) Agricultural Production Statistics Database (FAOSTAT), http://faostat.fao.org/
  8. Ganapathi TR, Higgs NS, Balint-Kurti PJ, Arntzen CJ, May GD, Van Eck JM (2001) Agrobacterium-mediated transformation of embryogenic cell suspensions of the banana cultivar Rasthali (ABB). Plant Cell Rep 20:157-162 https://doi.org/10.1007/s002990000287
  9. Harding RM, Burns TM, Hafner G, Dietzgen RG, Dale JL (1993) Nucleotide sequence of on component of the component of the banana bunchy top virus genome contains a putative replicase gene. J Gen Virol 74:323-328 https://doi.org/10.1099/0022-1317-74-3-323
  10. Hiei Y, Komari T, Kubo T (1997) Transformation of rice mediated by Agrobacterium tumefaciens. Plant Mol Biol 35:205-218 https://doi.org/10.1023/A:1005847615493
  11. Huang HE, Ger MJ, Yip MK, Chen CY, Pandey AK, Feng TY (2004) A hypersensitive response was induced by virulent bacteria in transgenic tobacco plants overexpressing a plant ferredoxin-like protein (PFLP). Physiol Mol Plant Pathol 64:103-110 https://doi.org/10.1016/j.pmpp.2004.05.005
  12. Huang HE, Ger MJ, Chen CY, Yip MK, Chung MC, Feng TY (2006) Plant ferredoxin-like protein (PFLP) exhibits an anti-microbial ability against soft-rot pathogen Erwinia carotovora subsp. carotovora in vitro and in vivo. Plant Sci 171:17-23 https://doi.org/10.1016/j.plantsci.2006.01.007
  13. Huang HE, Lee CA, Kuo CG, Chen HM, Ger MJ, Tsai YU, Chen YU, Lin MK, Feng TY (2007) Resistance enhancement of transgenic tomato to bacterial pathogens by the heterologous expression of sweet pepper ferredoxin-I protein (PFLP). Phytopathology 97:900-906 https://doi.org/10.1094/PHYTO-97-8-0900
  14. Hwang SC (2002) Application of tissue culture technology for controlling Fusarium wilt of banana. Plant Pathol Bull 11:57-61 (English abstract)
  15. Hwang S, Ko W (2004) Cavendish banana cultivars resistant to Fusarium wilt acquired through somaclonal variation in Taiwan. Plant Dis 88:580-588 https://doi.org/10.1094/PDIS.2004.88.6.580
  16. Khanna H, Becker D, Kleidon J, Dale J (2004) Centrifugation assisted Agrobacterium tumefaciens-mediated transformation (CAAT) of embryogenic cell suspensions of banana (Musa spp. Cavendish AAA and Lady finger AAB). Mol Breed 14:239-252 https://doi.org/10.1023/B:MOLB.0000047771.34186.e8
  17. Liau CH, You SJ, Prasad V, Hsiao HH, Lu JC, Yang NS, Chan MT (2003) Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid. Plant Cell Rep 21:993-998 https://doi.org/10.1007/s00299-003-0614-9
  18. Lichtenstein CP, Draper J (1985) Genetic engineering of plants. In: Glover DM (ed) DNA cloning: a practical approach, vol 2. IRL Press, Washington, DC, pp 78-119
  19. Lin HJ, Cheng HY, Chen CH, Huang HC, Feng TY (1997) Plant amphipathic proteins delay the hypersensitive response caused by harpinPss and Pseudomonas syringae pv. syringae. Physiol Mol Plant Pathol 51:367-376 https://doi.org/10.1006/pmpp.1997.0121
  20. May GD, Afza R, Mason HS, Wiecko A, Novak FJ, Arntzen CJ (1995) Generation of transgenic banana (Musa accuminata) plants via Agrobacterium mediated transformation. Biotechnology 13:486-492 https://doi.org/10.1038/nbt0595-486
  21. Molina AB, Fabregar E, Sinohin VG, Yi G, Viljoen A (2009) Recent occurrence of Fusarium oxysporum f. sp. cubense tropical race 4 in Asia. Acta Hortic 828:109-116
  22. Murashige T, Skoog F (1962)Arevisedmediumfor rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  23. Novak FJ, Afza R, Van Duren M, Perea-Dallos M, Conger BV, Tang X (1989) Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking (ABB) bananas (Musa spp.). Biotechnology 7:154-159 https://doi.org/10.1038/nbt0289-154
  24. Ploetz RC (2006) Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology 96:653-656 https://doi.org/10.1094/PHYTO-96-0653
  25. Sagi L, Remy S, Panis B, Swennen R, Volckaert G (1994) Transient gene expression in electroported banana (Musa spp. cultivar 'Bluggoe', ABB group) protoplasts isolated from regenerable embryogenic cell suspensions. Plant Cell Rep 13:262-266
  26. Sagi L, Panis B, Remy S, Schoofs H, De Smet K, Remy S, Swennen R, Cammue BPA (1995a) Genetic transformation of banana and plantain (Musa spp.) via particle bombardment. Biotechnology 13:481-485 https://doi.org/10.1038/nbt0595-481
  27. Sagi L, Remy S, Verelst B, Panis B, Cammue BPA, Volckaert G, Swennen R (1995b) Transient gene expression in transformed banana (Musa spp. cultivar 'Bluggoe') protoplasts and embryogenic cell suspension. Euphytica 85:89-95 https://doi.org/10.1007/BF00023934
  28. Sagi L, Remy S, Verelst B, Swennen R, Panis B (1995c) Stable and transient genetic transformation of banana (Musa spp.) protoplasts and cells. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Plant protoplasts and genetic engineering VI, vol 34. Springer, Berlin, pp 214-227
  29. Sambrook J, Fritsch EF, Maniatis T (1989) In: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 9.31-9.57
  30. Schenk PM, Sagi L, Remans T, Dietzgen RG, Bernard MJ, Graham MW, Manners JM (1999) A promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants. Plant Mol Bio 39:1221-1230 https://doi.org/10.1023/A:1006125229477
  31. Sherwood RT, Hagedorn DJ (1958) Determining common root rot potential of pea fields. Wis Agric Exp Stn Bull 531:12
  32. Smith RH, Hood E (1995) Agrobacterium tumefaciens transformation of monocotyledons. Crop Sci 35:301-309 https://doi.org/10.2135/cropsci1995.0011183X003500020001x
  33. Tang K, Sun X, Hu Q, Wu A, Lin CH, Lin HJ, Twyman RM, Christou P, Feng TY (2001) Transgenic rice plants expressing the ferredoxin-like protein (AP1) from sweet pepper show enhanced resistance to Xanthomonas oryzae pv oryzae. Plant Sci 160:1035-1042 https://doi.org/10.1016/S0168-9452(01)00351-X
  34. Tripathi L, Tripathi JN, Hughes JdA (2005) Agrobacterium-mediated transformation of plantain (Musa spp.) cultivar Agbagba. Afr J Biotechnol 4:1378-1383
  35. Tripathi L, Tripathi JN, Tushemereirwe WK (2008) Rapid and efficient production of transgenic East African Highland Banana (Musa spp.) using intercalary meristematic tissues. Afr J Biotechnol 7:1438-1445
  36. Tushemereirwe WK, Kangire A, Smith J, Ssekiwoko F, Nakyanzi M, Kataama D, Musiitwa C, Karyeija R (2003) An out break of banana bacterial wilt in Uganda. InfoMusa 12:2-6
  37. Van den Berg N, Berger D, Hein I, Birch PRJ, Wingfield MJ, Viljoen A (2007) Tolerance in banana to Fusarium wilt is associated with early up-regulation of cell wall-strengthening genes in the roots. Mol Plant Pathol 8:333-341 https://doi.org/10.1111/j.1364-3703.2007.00389.x
  38. Viljoen A (2002) The status of Fusarium wilt (Panama disease) of banana in South Africa. S Afr J Sci 98:341-344
  39. Yip MK, Huang HE, Ger MJ, Chiu SH, Tsai YC, Lin CI, Feng TY (2007) Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants. Plant Cell Rep 26:449-457 https://doi.org/10.1007/s00299-006-0246-y
  40. You SU, Liau CH, Huang HE, Feng TY, Prasad V, Hsiao HH, Lu JC, Chan MT (2003) Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation. Planta 217:61-65

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  15. Genetically engineered bananas-From laboratory to deployment vol.175, pp.3, 2011, https://doi.org/10.1111/aab.12538
  16. A simple and rapid protocol for the genetic transformation of Ensete ventricosum vol.15, pp.1, 2011, https://doi.org/10.1186/s13007-019-0512-y
  17. The application of the ‘Gene-deletor’ technology in banana vol.140, pp.1, 2011, https://doi.org/10.1007/s11240-019-01714-3
  18. A comprehensive strategy for managing Fusarium wilt of banana vol.1272, pp.None, 2011, https://doi.org/10.17660/actahortic.2020.1272.9
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  20. Improvements in the Resistance of the Banana Species to Fusarium Wilt: A Systematic Review of Methods and Perspectives vol.7, pp.4, 2011, https://doi.org/10.3390/jof7040249
  21. The effect of a consortium of Penicillium sp. and Bacillus spp. in suppressing banana fungal diseases caused by Fusarium sp. and Alternaria sp. vol.131, pp.4, 2011, https://doi.org/10.1111/jam.15067