Plant Biotechnology Reports

  • 제5권2호
  • /
  • Pages.169-175
  • /
  • 2011
  • /
  • 1863-5466(pISSN)
  • /
  • 1863-5474(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

The use of JIP test to evaluate drought-tolerance of transgenic rice overexpressing OsNAC10

  • Redillas, Mark C.F.R. (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Strasser, Reto J. (University of Geneva) ;
  • Jeong, Jin-Seo (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Kim, Youn-Shic (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Kim, Ju-Kon (School of Biotechnology and Environmental Engineering, Myongji University)
  • 투고 : 2010.12.08
  • 심사 : 2011.01.01
  • 발행 : 2011.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, the JIP test was exploited to assess drought-tolerance of transgenic rice overexpressing OsNAC10. Two types of promoters, RCc3 (root-specific) and GOS2 (constitutive), were used to drive the transcription factor OsNAC10, a gene involved in diverse functions including stress responses. Three-month-old plants were exposed to drought for 1 week and their fluorescence kinetics was evaluated. Our results showed that drought-treated non-transgenic plants (NT) have higher fluorescence intensity at the J phase (2 ms) compared to transgenic plants, indicating a decline in electron transport beyond the reduced plastoquinone ($Q_A^-$). As manifested by negative L bands, transgenic plants also showed higher energetic connectivity and stability over NT plants under drought conditions. Also, the pool size of the end electron acceptor at the photosystem I was reduced more in NT than in transgenic plants under drought conditions. Furthermore, the transgenic plants had higher $PI_{total}$, a combined parameter that reflects all the driving forces considered in JIP test, than NT plants under drought conditions. In particular, the $PI_{total}$ of the RCc3:OsNAC10 plants was higher than that of NT plants, which was in good agreement with their differences in grain yield. Thus, the JIP test proved to be practical for evaluating drought-tolerance of transgenic plants.

키워드

참고문헌

  1. Baker NR, Oxborough K (2004) Chlorophyll fluorescence as a probe of photosynthetic productivity. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, Dordrecht, pp 65-82.
  2. Baker NR, East TM, Long SP (1983) Chilling damage to photosynthesis in young Zea mays. J Exp Bot 34:189-197. https://doi.org/10.1093/jxb/34.2.189
  3. Ballottari M, Dall'Osto L, Morosinotto T, Bassi R (2007) Contrasting behavior of higher plant photosystem I and II antenna systems during acclimation. J Biol Chem 282:8947-8958. https://doi.org/10.1074/jbc.M606417200
  4. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Mol Breed 5:493-503.
  5. Chernev P, Golstev V, Zaharieva I, Strasser RJ (2006) A highly restricted model approach quantifying structural and functional parameters of photosystem II probed by the chlorophyll a fluorescence rise. Ecol Eng Prot 2:19-29.
  6. Garg AK, Kim JK, Owens TG, Ranwala AP, Choi YD, Kochian LV, Wu RJ (2002) Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc Natl Acad Sci USA 99:15898-15903. https://doi.org/10.1073/pnas.252637799
  7. Govindjee (1995) Sixty-three years since Kautsky: chlorophyll a fluorescence. Aust J Plant Physiol 22:131-160. https://doi.org/10.1071/PP9950131
  8. Haldiman P, Strasser RJ (1999) Effects of anaerobiosis as probed by the polyphasic chlorophyll a fluorescence rise kinetic in pea (Pisum sativum L.). Photosynth Res 62:67-83. https://doi.org/10.1023/A:1006321126009
  9. Haup-Herting S, Fock HP (2000) Exchange of oxygen nd its role in energy dissipation during drought stress in tomato plants. Physiol Plant 110:489-495. https://doi.org/10.1111/j.1399-3054.2000.1100410.x
  10. Horton P, Ruban AV, Walters RG (1996) Regulation of light harvesting in green plants. Annu Rev Plant Physiol Plant Mol Biol 47:655-684. https://doi.org/10.1146/annurev.arplant.47.1.655
  11. Ismaelov MA, Zulfugarov IS, Aliev JA (1998) The effect of water deficit on wheat thylakoid membranes. ISPT congress abstract
  12. Ito Y, Kastura Y, Maruyama K, Taji T, Kobayashi M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2006) Functional analysis of DREB1/CBF-type transcription factors involve in coldresponsive gene expression in transgenic rice. Plant Cell Physiol 47:141-153.
  13. Jang IC, Nahm BH, Kim JK (1999) Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system. Mol Breed 5:453-461. https://doi.org/10.1023/A:1009665314850
  14. Jeong JS, Park YT, Jung H, Park SH, Kim JK (2009) Rice NAC proteins act as homodimers and heterodimers. Plant Biotechnol Rep 3:127-134. https://doi.org/10.1007/s11816-009-0081-z
  15. Jeong JS, Kim YS, Baek KH, Jung HR, Ha SH, Choi YD, Kim MK, Reuzeau C, Kim JK (2010) Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant Physiol 153:185-197. https://doi.org/10.1104/pp.110.154773
  16. Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis- the basics. Annu Rev Plant Physiol Plant Mol Biol 42:313-349. https://doi.org/10.1146/annurev.pp.42.060191.001525
  17. Lazar D, Llik P, Naus J (1997) An appearance of K-peak in fluorescence induction depends on the acclimation of barley leaves to higher temperatures. J Lumin 72-74:595-596.
  18. Long SP, Humphries SW, Falkowski PG (1994) Photoinhibition of photosynthesis in nature. Annu Rev Plant Physiol Mol Biol 45:633-662. https://doi.org/10.1146/annurev.pp.45.060194.003221
  19. Munday JCM, Govindjee (1969) Light-induced changes in the fluorescence yield of chlorophyll a in vivo III. The dip and peak in the fluorescence transient off Chlorella pyrenoidosa. Biophys J 9:1-21.
  20. Nakashima K, Trans LS, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi- Shinozaki K (2007) Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-response gene expression in rice. Plant J 51:617-630. https://doi.org/10.1111/j.1365-313X.2007.03168.x
  21. Oh SJ, Kim Ys, Kwon CW, Park HK, jeong JS, Kim JK (2009) Overexpression of transcription factor AP37 in rice improves grain yield under drought conditions. Plant Physiol 150:1368-1379. https://doi.org/10.1104/pp.109.137554
  22. Oukarroum A, Madidi SE, Schansker G, Strasser RJ (2007) Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and rewatering. Environ Exp Bot 60:438-446. https://doi.org/10.1016/j.envexpbot.2007.01.002
  23. Rohacek K, Bartak M (1999) Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. Photosynthetica 37:339-363. https://doi.org/10.1023/A:1007172424619
  24. Sayed OH (2003) Chlorophyll fluorescence as a tool in cereal crop research. Photosynthetica 41:321-330.
  25. Smit MF, Van Heerden PDR, Pienaar JL, Weissflog L, Strasser RJ, Kruger GHJ (2009) Effect of trifluoroacetate, a persistent degradation product of fluorinated hydrocarbons, on C3 and C4 crop plants. Plant Physiol Biochem 47:623-634. https://doi.org/10.1016/j.plaphy.2009.02.003
  26. Strasser RJ (1978) The grouping model of plant photosynthesis. In: Akoyunoglou G, Argyroudi-Akoyunoglou JH (eds) Chloroplast development. Elsevier/North-Holland Biomedical Press, New York, pp 513-524.
  27. Strasser RJ (1981) The grouping model of plant photosynthesis: heterogeneity of photosynthetic units in thylakoids. In: Akoyunoglou G (ed) Photosynthesis III. Structure and molecular organization of the photosynthetic apparatus. Balaban International Science Services, Philadelphia.
  28. Strasser RJ, Tsimilli-Michael M, Dangre D, Rai M (2007) Biophysical phenomics reveals functional building blocks of plants systems biology: a case study for the evaluation of the impact of mycorrhization with Piriformospora indica. In: Varma A, Oelmuler R (eds) Advanced techniques in soil microbiology. Soil biology series, vol I. Springer, Berlin, pp 319-341.
  29. Strasser RJ, Srivastava A, Govindjee (1995) Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol 61:32-42. https://doi.org/10.1111/j.1751-1097.1995.tb09240.x
  30. Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, Dordrecht, pp 321-362.
  31. Tezara W, Mitchell VJ, Driscoll SO, Lawlor OW (1999) Water stressinhibits plant photosynthesis by decreasing coupling factor andATP. Nature 401:914-917. https://doi.org/10.1038/44842
  32. Van Heerden PDR, Strasser RJ, Kru¨ger GHJ (2004)Reduction of dark chilling stress in N2-fixing soybean by nitrate as indicated by chlorophyll a fluorescence kinetics. Physiol Plant 121:239-249. https://doi.org/10.1111/j.0031-9317.2004.0312.x
  33. Vincour B, Altman A (2005) Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Opin Biotechnol 16:123-132. https://doi.org/10.1016/j.copbio.2005.02.001
  34. Xu D, Duan X, Wang B, Hong B, Ho T, Wu R (1996) Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol 110:249-257.
  35. Yordanov I, Velikova V, Tsonev T (2000) Plant responses to drought, acclimation, and stress tolerance. Photosynthetica 38:171-186. https://doi.org/10.1023/A:1007201411474
  36. Yusuf MA, Kumar D, Rajwanshi R, Strasser RJ, Tsimilli-Michael M, Govindjee Sarin NB (2010) Overexpression of c-tocopherol methyl transferase gene in transgenic Brassica juncea plants alleviates abiotic stress: physiological and chlorophyll a fluorescence measurements. Biochim Biophys Acta 1797:1428-1438. https://doi.org/10.1016/j.bbabio.2010.02.002
  37. Zhu XG, Govindjee, Baker NR, deSturler E, Ort DR, Long SP (2005) Chlorophyll a fluorescence induction kinetics in leaves predicted from a model describing each discrete step of excitation energy and electron transfer associated with Photosystem II. Planta 223:114-133. https://doi.org/10.1007/s00425-005-0064-4
  38. Zubek S, Turnau K, Tsimilli-Michael M, Strasser RJ (2009) Response of endangered plant species to inoculation with arbuscular mycorrhizal fungi and soil bacteria. Mycorrhiza 19:113-123. https://doi.org/10.1007/s00572-008-0209-y

피인용 문헌

  1. How genetic modification of roots affects rhizosphere processes and plant performance vol.63, pp.9, 2012, https://doi.org/10.1093/jxb/err399
  2. Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice vol.159, pp.3, 2011, https://doi.org/10.1104/pp.112.194928
  3. Chlorophyll a Fluorescence Transient Analysis of Transgenic Potato Overexpressing D-galacturonic Acid Reductase Gene for Salinity Stress Tolerance vol.53, pp.4, 2011, https://doi.org/10.1007/s13580-012-0035-1
  4. OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field vol.11, pp.1, 2011, https://doi.org/10.1111/pbi.12011
  5. Characterization of the stress-inducible OsNCED3 promoter in different transgenic rice organs and over three homozygous generations vol.237, pp.1, 2011, https://doi.org/10.1007/s00425-012-1764-1
  6. Photosynthetic acclimation to drought stress in Agave salmiana Otto ex Salm-Dyck seedlings is largely dependent on thermal dissipation and enhanced electron flux to photosystem I vol.122, pp.1, 2014, https://doi.org/10.1007/s11120-014-0008-6
  7. Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane vol.53, pp.3, 2015, https://doi.org/10.1007/s11099-015-0111-8
  8. Impact of Cuscuta australis infection on the photosynthesis of the invasive host, Mikania micrantha, under drought condition vol.15, pp.4, 2011, https://doi.org/10.1111/wbm.12077
  9. Overexpression of the OsbZIP66 transcription factor enhances drought tolerance of rice plants vol.11, pp.1, 2011, https://doi.org/10.1007/s11816-017-0430-2
  10. Expression Analysis of Sound Vibration-Regulated Genes by Touch Treatment in Arabidopsis vol.8, pp.None, 2017, https://doi.org/10.3389/fpls.2017.00100
  11. Chlorophyll fluorescence as a tool for nutrient status identification in rapeseed plants vol.136, pp.3, 2011, https://doi.org/10.1007/s11120-017-0467-7
  12. Decrease in the Photosynthetic Performance of Temperate Grassland Species Does Not Lead to a Decline in the Gross Primary Production of the Ecosystem vol.9, pp.None, 2011, https://doi.org/10.3389/fpls.2018.00067
  13. Overexpression of OsNAC14 Improves Drought Tolerance in Rice vol.9, pp.None, 2011, https://doi.org/10.3389/fpls.2018.00310
  14. Responses of growth and photosynthetic fluorescent characteristics in Ottelia acuminata to a water-depth gradient vol.33, pp.1, 2011, https://doi.org/10.1080/02705060.2018.1443841
  15. Differential ecophysiological responses and resilience to heat wave events in four co-occurring temperate tree species vol.13, pp.6, 2018, https://doi.org/10.1088/1748-9326/aabcd8
  16. Causes of reduced leaf‐level photosynthesis during strong El Niño drought in a Central Amazon forest vol.24, pp.9, 2011, https://doi.org/10.1111/gcb.14293
  17. Effects of drought stress on photosynthesis and photosynthetic electron transport chain in young apple tree leaves vol.7, pp.11, 2018, https://doi.org/10.1242/bio.035279
  18. Overexpression of OsTF1L, a rice HD‐Zip transcription factor, promotes lignin biosynthesis and stomatal closure that improves drought tolerance vol.17, pp.1, 2019, https://doi.org/10.1111/pbi.12951
  19. Adaptation of photosynthesis to water deficit in the reproductive phaseof a maize (Zea mays L.) inbred line vol.57, pp.2, 2011, https://doi.org/10.32615/ps.2019.047
  20. Selenium improves the transport dynamics and energy conservation of the photosynthetic apparatus of in vitro grown Billbergia zebrina (Bromeliaceae) vol.57, pp.4, 2011, https://doi.org/10.32615/ps.2019.105
  21. Salinity variation effects on photosynthetic responses of the mangrove species Rhizophora mangle L. growing in natural habitats vol.57, pp.4, 2011, https://doi.org/10.32615/ps.2019.121
  22. Characterization of Photosynthetic Phenotypes and Chloroplast Ultrastructural Changes of Soybean ( Glycine max ) in Response to Elevated Air Temperatures vol.11, pp.None, 2011, https://doi.org/10.3389/fpls.2020.00153
  23. Effects of Nitrogen Fertilization on Forage Production and Nutritive Nalue of Geukdong 6, Teosinte Hybrid [Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis] vol.40, pp.1, 2011, https://doi.org/10.5333/kgfs.2020.40.1.37
  24. Special issue in honour of Prof. Reto J. Strasser - Can be performance indexes used to select plant growth-promoting rhizobacteria? vol.58, pp.spec, 2011, https://doi.org/10.32615/ps.2019.149
  25. Special issue in honour of Prof. Reto J. Strasser - Origin rather than mild drought stress influenced chlorophyll a fluorescence in contrasting silver fir (Abies alba Mill.) provenances vol.58, pp.spec, 2011, https://doi.org/10.32615/ps.2020.011
  26. Implications of intra-seasonal climate variations on chlorophyll a fluorescence and biomass in winter barley breeding program vol.58, pp.4, 2011, https://doi.org/10.32615/ps.2020.053
  27. Heterologous activation of the Hevea PEP16 promoter in the rubber-producing laticiferous tissues of Taraxacum kok-saghyz vol.10, pp.None, 2020, https://doi.org/10.1038/s41598-020-67328-4
  28. Differential response of photosystem II and I photochemistry in leaves of two Crambe abyssinica Hochst lineages submitted to water deficit vol.58, pp.5, 2011, https://doi.org/10.32615/ps.2020.065
  29. Disentangling the photosynthesis performance in japonica rice during natural leaf senescence using OJIP fluorescence transient analysis vol.48, pp.2, 2011, https://doi.org/10.1071/fp20104
  30. Impact of saline solution on growth and photosystem II during in vitro cultivation of Bromelia antiacantha (Bromeliaceae) vol.72, pp.None, 2011, https://doi.org/10.1590/2175-7860202172018
  31. Comparative Study of Drought Stress Effects on Traditional and Modern Apple Cultivars vol.10, pp.3, 2011, https://doi.org/10.3390/plants10030561
  32. Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice vol.22, pp.14, 2011, https://doi.org/10.3390/ijms22147656
  33. Analyses of OJIP transients in leaves of two epiphytic orchids under drought stress vol.27, pp.4, 2021, https://doi.org/10.1590/2447-536x.v27i4.2334
  34. PSII photochemistry responses to drought stress in autochthonous and modern sweet cherry cultivars vol.59, pp.4, 2011, https://doi.org/10.32615/ps.2021.045
  35. Photosynthetic acclamatory response of Panicum antidotale Retz. populations to root zone desiccation stress vol.84, pp.None, 2024, https://doi.org/10.1590/1519-6984.252735