한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제19권3호
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  • Pages.177-184
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  • 2011
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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짚신나물 물 추출물의 항산화 활성 및 미백효과에 관한 연구

Antioxidant and Whitening Effects of Agrimonia pilosa Ledeb Water Extract

  • 김태혁 (강원대학교 생명건강공학과) ;
  • 김정미 (강원대학교 생명건강공학과) ;
  • 백종미 (강원대학교 생명건강공학과) ;
  • 김태우 (강원대학교 생명건강공학과) ;
  • 김대중 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 박정해 (화진화장품 기술연구소) ;
  • 최면 (강원대학교 생명건강공학과)
  • Kim, Tae-Hyuk (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Jeong-Mi (Department of Bio-Health Technology, Kangwon National University) ;
  • Baek, Jong-Mi (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Tae-Woo (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Dae-Jung (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Park, Jeong-Hae (Hwajin Cosmetic R&D Center) ;
  • Choe, Myeon (Department of Bio-Health Technology, Kangwon National University)
  • 투고 : 2011.02.16
  • 심사 : 2011.06.17
  • 발행 : 2011.06.30
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초록

This study was performed to assess the antioxidant activities and whitening effects of Agrimonia pilosa Ledeb on melanin synthesis. The whitening effects of Agrimonia pilosa Ledeb water extracts were examined by in vitro mushroom tyrosinase assay and B16BL6 melanoma cells. We assessed inhibitory effect of Agrimonia pilosa Ledeb water extract on expression of melanogenic enzyme proteins including tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2) in B16BL6 cells. Inhibitory effect of Agrimonia pilosa Ledeb onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activitie. Our results indicated that Agrimonia pilosa Ledeb water extract effectively inhibited free radical generation. In DPPH and hydroxy radical scavenging activity, Agrimonia pilosa Ledeb water extract had a potent anti-oxidant activity in a dose-dependent manner. They significantly inhibited tyrosinase activity in vitro and in B16BL6 melanoma cells. Also, Agrimonia pilosa Ledeb suppressed the expression of tyrosinase in B16BL6 melanoma cells. These results show that Agrimonia pilosa Ledeb inhibited melanin production on the melanogenesis. The underlying mechanism of Agrimonia pilosa Ledeb on whitening activity may be due to the inhibition of tyrosinase activity. We suggest that Agrimonia pilosa Ledeb may be useful as new natural active ingredients for antioxidant and whitening cosmetics.

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참고문헌

  1. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 26:1199-1200.
  2. Boissy RE, Sakai C, Zhao H, Kobayashi T and Hearing VJ. (1998). Human tyrosinase related protein-1(TRP-1) does not function as a DHICA oxidase activity in contrast to murine TRP-1. Experimental Dermatology. 7:198-204. https://doi.org/10.1111/j.1600-0625.1998.tb00324.x
  3. Braca A, De Tommasi N, Di Bari L, Pizza C, Politi M and Morelli I. (2001). Antioxidant principles form Bauhinia terapotensis. Journal of Nature Products. 64:892-895. https://doi.org/10.1021/np0100845
  4. Chang MS, Kim HM, Yang WM, Kim DR, Park EH, Ko EB, Choi MJ, Kim HY, Oh JH, Shim KJ, Yoon JW and Park SK. (2007). Inhibitory effects of Nelumbo nucifera on tyrosinase activity and melanogenesis in clone M-3 melanocyte cells. Korea Journal of Herbology. 22:87-94.
  5. Cho WG. (2007). Comparision of drug delivery using hairless and pig skin. Journal of Korea Oil Chemists' Society. 24: 410-415
  6. Choe M, Kim DJ, Lee HJ, You JK, Seo DJ, Lee JH and Chung MJ. (2008). A study on the glucose-regulating enzymes and antioxidant activities of water extracts from medicinal herbs. Journal of Korean Society Food Science Nutrition. 37:542-547.
  7. Chung MJ, Sung NJ, Park CS, Kweon DK, Mantovani A, Moon TW, Lee SJ and Park KH. (2008). Antioxidative and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2 cells and in C57BL/6J mice. European Journal of Pharmaceutical Science. 578:159-170. https://doi.org/10.1016/j.ejphar.2007.09.036
  8. Chung MJ, Walker PA, Brown RW and Hogstr C. (2005). Zinc-mediated gene expression offers protection against H2O2- induced cytotoxicity. Toxicology and Applied Pharmacology. 205:225-236. https://doi.org/10.1016/j.taap.2004.10.008
  9. Jang SH, Yu EA, Han KS, Shin SC, Kim HK and Lee SG. (2008). Changes in total polyphenol contents and DPPH radical scavenging activity of Agrimonia pilosa according to harvest time and various part. Korean Journal of Medicinal Crop Science. 16:397-401
  10. Kim BM, Jun JY, Park YB and Jeong IH. (2006). Antioxidative activity of methanolic extracts from seaweeds. Journal of Korean Society Food Science Nutrition. 35:1097-1101 https://doi.org/10.3746/jkfn.2006.35.8.1097
  11. Kim HJ, Lim HW, Kim BH, Kim HS, Choi SW and Yoon CS. (2006). Studies on the anti-acne effect of Agrimonia pilosa Ledeb. Journal of Society of Cosmetic Scientists of Korea. 32:53-58
  12. Kim YM. (2010). The inhibitory effect of Agrimonia pilosa Ledeb extract on allergic reaction. Korean Journal of Medicinal Crop Science. 18:398-404
  13. Motogawa T. (2000). Development of new skin whitening agents. Inhibition of $\alpha$-MSH induced melanogenesis by Sophorae radix extracts. Fragrance Journal. 9:38-42.
  14. Negroiu G, Branza-Nichita N, Petrescu AJ, Dwek RA and Petrescu SM. (1999). Protein specific N-glycosylation of tyrosinase and tyrosinase-related protein-1 in B16 mouse melanoma cells. Journal of Biochemical Society. 344:659-665. https://doi.org/10.1042/0264-6021:3440659
  15. Oh HC, Lim KS, Hwang CY, Youn IH and Kim NK. (2007). A study on the melanin synthesis inhibition and whitening effect of Bombysis corpus. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 20:1-13.
  16. Park SH, Lee BY, Han CS, Kim JG, Kim KT, Kim KH and Kim YH. (2008). Whitening effect of Angelica keiskei Koidzumi extract by inhibition of endothelin-1 production and melanogenesis. Journal of Society Cosmetic Scientists Korea. 34:101-107.
  17. Park SH, Lee BY, Lee SH, Han CS, Kim JG, Kim KT, Kim KH and Kim YH. (2009). Whitening effect of dayflower (Commelina communis L.) extract by inhibition of N-linked glycosylation process and melanogenesis. Journal of Society Cosmetic Scientists Korea. 35:73-78.
  18. Prota G. (1990). Recent advances in the chemistry of melanogenesis in mammals. Journal of Investive Dermatology. 75:122-128.
  19. Ra KS, Suh HJ, Chung SH and Son JY. (1997). Antioxidant activity of solvent extract from onion skin. Korean Journal of Food Science Technology. 29: 595-601.
  20. Ryu SH, Jeon YS, Kwon MJ, Moon JW, Lee YS and Moon GS. (1997). Effect of kimchi extracts to reactive oxygen species in skin cell cytotoxicity. Journal of Korean Society Food Science Nutrition. 26: 814-821.
  21. Seiberg M, Paine C, Sharlow E, Andrade-Gordon P, Costanzo M, Eisinger M and Shapiro SS. (2000). The proteaseactivated receptor 2 regulates pigmentation via keratinocytemelanocyte interactions. Experimental Cell Research. 254:25-32. https://doi.org/10.1006/excr.1999.4692
  22. Shin JY. (2001). Screening of natural products that have activities against skin-aging. Korean Journal of Food Nutrition. 14:568-572.
  23. Yang H, Zheng S, Meijer L, Li SH, Leclerc S, Yu LL, Cheng JQ and Zhang SZ. (2005). Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cde25 tyrosine phosphatase, an activator of the mitosis-inducing p34cde2 Kinase. Journal of Zhejiang Univ. Science 6:656-663. https://doi.org/10.1631/jzus.2005.A0656
  24. Yoo YG, Joung MS, Choi JW and Kim JH. (2005). The study on the whitening effect of Ephedra sinica extract. Journal of Society Cosmetic Scientists Korea. 31:153-159.
  25. You JK, Chung MJ, Kim DJ, Seo DJ, Park JH, Kim TW andChoe M. (2009). Antioxidant and tyrosinase inhibitory effects of Paeonia suffruticosa water extract. Journal of Korean Society Food Science Nutrition. 38:292-296. https://doi.org/10.3746/jkfn.2009.38.3.292
  26. Zhang R, Lee HJ, Yoon YG, Kim SM, Kim HS, Li SH and An SK. (2009). The melanin inhibition, anti-aging and antiinflammation effects of Portulaca oleracea extracts on cells. Korean Society for Biotechnology and Bioengineering Journal. 24:397-402.

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  8. Whitening and Anti-wrinkle Effects of Korean Native Dendrobium moniliforme Methanol Extract vol.22, pp.5, 2014, https://doi.org/10.7783/KJMCS.2014.22.5.331
  9. Anti-melanogenesis and Anti-wrinkle Properties of Korean Native Dendrobium speciosum Ethanol Extract vol.29, pp.2, 2016, https://doi.org/10.7732/kjpr.2016.29.2.155
  10. Inhibitory Effect ofAgrimonia PilosaLeaf Extract on the UV-Induced Photoaging-Related Ion Channel, ORAI1, and the Enzymes Tyrosinase and Elastase vol.40, pp.1, 2016, https://doi.org/10.1111/jfbc.12171
  11. Applicability of Lindera obtusiloba Flower Extracts as Cosmetic Ingredients vol.15, pp.2, 2017, https://doi.org/10.20402/ajbc.2016.0088