약학회지 (YAKHAK HOEJI)

  • 제55권1호
  • /
  • Pages.64-68
  • /
  • 2011
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  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

황련해독탕 중 3종 생리활성 물질의 HPLC-DAD 동시 정량분석법 확립

Simultaneous Analysis of three Marker Components in Hwangryunhaedok-tang by HPLC-DAD

  • Yang, Hye-Jin (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Weon, Jin-Bae (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Jin-Yeul (TKM Converging Research Division, Korea Institute of Oriental Medicine) ;
  • Ma, Choong-Je (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University)
  • 투고 : 2010.09.20
  • 심사 : 2011.02.11
  • 발행 : 2011.02.28
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초록

In this study, a high performance liquid chromatography-diode array detector method was established, for simultaneous determination of three compounds, berberine, palmatine and geniposide in Hwangryunhaedok-tang, To develop and validate method, $C_{18}$ column (5 ${\mu}M$, 4.6 mm${\times}$250 mm) was used with gradient mobile phase, water containing 0.1% trifluoroacetic acid (TFA) and MeOH at the column temperature of $30^{\circ}C$. UV wavelength was set at 230 and 280 nm. Validation of the chromatography method was evaluated by linearity, precision and accuracy test. Calibration curve of standard components showed good linearity ($R^2$ > 0.9999). The limits of detection (LOD) and limits of quantification (LOQ) varied from 0.05 to 0.17 ${\mu}g/ml$ and 0.15 to 0.53 ${\mu}g/ml$, respectively. The relative standard deviations (RSDs) data of intra-day and inter-day test were in less than 2.99% and 1.90%, respectively. The results of the accuracy test were in the range of 98.36 to 102.52% with RSDs values 0.32 to 1.98%. The results of validation indicated that this method was a very accurate and sensitive assay.

키워드

참고문헌

  1. Normile, D. : Asian medicine. The new face of traditional Chinese medicine. Science 299, 188 (2003). https://doi.org/10.1126/science.299.5604.188
  2. Jiang, W.-Y. : Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science. Trends. Pharmacol. Sci. 26, 558 (2005). https://doi.org/10.1016/j.tips.2005.09.006
  3. Wang, Z. G. and Ren, J. : Current status and future direction of Chinese herbal medicine. Trends. Pharmacol. Sci. 23, 347 (2003).
  4. Lu, A. P., Jia, H. W., Xiao, C. and Lu, Q. P. : Theory of traditional Chinese medicine and therapeutic method of diseases. World J. Gastroenterol. 10, 1854 (2004). https://doi.org/10.3748/wjg.v10.i13.1854
  5. Kong, M. J., Ha, N. N., Lee, H. Y., Kim, Y. T., Rho, S. J. and Kim, H. C. : Effect of Hwangryunhaedok-tang and its modified prescription on the recovery of spatial cognitive function in the brain ischemia induced by four-vessel occlusion in rats. Kor. J. Herbology 19, 161 (2004).
  6. Hwang, Y. S., Shin, C. Y., Huh, Y. and Ryu, J. H. : Hwangryun- Hae-Dok-tang (Huanglian-Jie-Du-Tang) extract and its constituents reduce ischemia-reperfusion brain injury and neutrophil infiltration in rats. Life Sci. 71, 2105 (2002). https://doi.org/10.1016/S0024-3205(02)01920-3
  7. Park, Y.-K., Jung, H.-W., Kim, C.-M., Choi, J.-S. and Kim, Y.-S. : Effects of Berberine on the production of inflammatory mediators from LPS-stimulated BV2 microglial cells. Kor. J. Herbology 22, 117 (2007).
  8. Lee, J.-G., Choi, J.-Y., Oh, J.-S., Jung, H.-W., Choi, E.-H., Lee, H.-S, Kim, J.-A., Chang, T.-S., Son, J.-K. and Lee, S.-H. : Isolation of melanin biosynthesis inhibitory compounds from the phellodendri cortex. Kor. J. Pharmacogn. 38, 387 (2007).
  9. Kim, G.-W. and Chung, M.-H. : Protective effects of geniposide and extract of Korean gardeniae fructus - on hepatic injury induced by toxic drugs in rats-. Kor. J. Pharmacogn. 25, 368 (1994).
  10. Klimek, B., Olszewska, M. A. and Tokar, M. : Simultaneous determination of flavonoids and phenylethanoids in the flowers of Verbascum densiflorum and V. phlomoides by highperformance liquid chromatography. Phytochem. Anal. 21, 150 (2009).
  11. Wei, H., Sun, L., Tai, Z., Gao, S., Xu, W. and Chen, W. : A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera. Anal. Chim. Acta. 662, 97 (2010). https://doi.org/10.1016/j.aca.2009.12.039
  12. Ryu, S. K., Jeon, J.-E., Kang, G. W., Kang, S. S. and Shin, J. H. : Simultaneous analysis of bioactive metabolites from Lonicera japonica flower buds by HPLC-DAD-MS/MS. Yakhak Hoeji 52, 446 (2008).
  13. Cao, X.-Y. and Wang, Z.-Z. : Simultaneous determination of four iridoid and secoiridoid glycosides and comparative analysis of Radix Gentianae Macrophyllae and their related substitutes by HPLC. Phytochem. Anal. 21, 348 (2010). https://doi.org/10.1002/pca.1206