Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Intestinal Growth and Development of Weanling Pigs in Response to Dietary Supplementation of Antibiotics, Phytogenic Products and Brewer's Yeast plus Bacillus Spores

  • Lee, C.-Young (Regional Animal Industry Center, Gyeongnam National University of Science and Technology) ;
  • Lim, Jung-Won (Regional Animal Industry Center, Gyeongnam National University of Science and Technology) ;
  • Ko, Young-Hyun (Regional Animal Industry Center, Gyeongnam National University of Science and Technology) ;
  • Kang, Sun-Young (Regional Animal Industry Center, Gyeongnam National University of Science and Technology) ;
  • Park, Man-Jong (Regional Animal Industry Center, Gyeongnam National University of Science and Technology) ;
  • Ko, Tae-Gu (Bio Resource Institute) ;
  • Lee, Ji-Hoon (Bio Resource Institute) ;
  • Hyun, Young (Bio Resource Institute) ;
  • Jeong, Kyu-Sik (Department of Pathology, College of Veterinary Medicine, Kyungpook National University) ;
  • Jang, In-Surk (Regional Animal Industry Center, Gyeongnam National University of Science and Technology)
  • Received : 2011.03.28
  • Accepted : 2011.06.17
  • Published : 2011.06.30
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Abstract

A total of 96 crossbred weanling barrows aged 21 days were randomly allocated to 32 pens of a new nursery to investigate the effects of antibiotics, phytogenics, and probiotics on intestinal growth and development. The animals were fed a set of three-phase basal diets containing 0.3% zinc oxide (CON) or the basal diets supplemented with 353 ppm of a combination of tiamulin, neomycin, chlortetracycline, and oxytetracycline (ANTI), 75 ppm triterpenoid saponin plus 150 ppm mixed saccharides (HERB; Sacchapin$^{(R)}$), or $1{\times}10^7$ brewer's yeasts plus $8{\times}10^7$ spores of each of Bacillus licheniformis and Bacillus subtilis per kilogram feed (PROBIO; Yeasture Plus 2B$^{(R)}$) for five weeks. Thirty-two pigs representing as many pens were slaughtered at the end of the feeding trial, after which morphological measures and digestive enzyme activities of intestinal mucosa were determined. Weight gain and gain:feed of the pigs were not affected by the dietary treatments (TRT) during the overall feeding trial. Total intestinal length was greater in PROBIO than in ANTI (P<0.05). Wet mucosa weight of the duodenum was not affected by TRT. However, jejunal mucosa weight was greater in PROBIO than in any other group sum of mucosa weights of the duodenum and jejunum was greater (P<0.05) in PROBIO than in ANTI and HERB. The height and width of duodenal villus were not affected by TRT, but crypt depth decreased (P<0.05) in response to HERB and PROBIO vs CON. Specific activities of alkaline phosphatase, sucrase, maltase, lactase, and leucine aminopeptidase in the duodenum and jejunum were not changed by TRT. In conclusion, results suggest that the present dietary treatments have no effects on growth performance of weanling pigs and that of PROBIO enhances intestinal growth and development under a clean experimental setting.

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References

  1. Alexopoulos, C., Georgoulaskis, I. E., Tzivara, A., Kyriakis, C. S., Govaris, A. and Kyriakis, S. C. 2004. Field evaluation of the effect of a probiotic-containing Bacillus licheniformis and Bacillus subtilis spores on the health status, performance, and carcass quality of grower and finisher pigs. J. Vet. Med. A. Physiol. Pathol. Clin. Med. 51:306-312. https://doi.org/10.1111/j.1439-0442.2004.00637.x
  2. Broom, L. J., Miller, H. M., Kerr, K. G. and Knapp, J. S. 2006. Effects of zinc oxide and Enterococcus faecium SF68 dietary supplementation on the performance, intestinal microbiota and immune status of weaned piglets. Res. Vet. Sci. 80:45-54. https://doi.org/10.1016/j.rvsc.2005.04.004
  3. Colina, J. J., Lewis, A. J., Miller, P. S. and Fischer, R. L. 2001. Dietary manipulation to reduce aerial ammonia concentrations in nursery pig facilities. J. Anim. Sci. 79:3096-3103. https://doi.org/10.2527/2001.79123096x
  4. Collington, G. K., Parker, D. S. and Armstrong, D. G. 1990. The influence of inclusion of either an antibiotic and a probiotic in the diet on the development of digestive enzyme activity in the pig. Br. J. Nutr. 64:59-70. https://doi.org/10.1079/BJN19900009
  5. Cowan, M. M. 1999. Plant products as antimicrobial products. Clin. Microbiol. Rev. 12:564-582.
  6. Cromwell, G. L. 2001. Antimicrobial and promicrobial agents. In: Lewis, A. J. and Southern, L. L. (Eds.), Swine Nutrition, second ed. CRC Press, Boca Raton, FL, USA, pp. 401-426.
  7. Dahlqvist, A. 1968. Assay of the intestinal disaccharidase. 1968. Anal. Biochem. 22:99-107. https://doi.org/10.1016/0003-2697(68)90263-7
  8. Davis, M. E., Parrott, T., Brown, D. C., de Rodas, B. Z., Johnson, Z. B, Maxwell, C. V. and Rehberger, T. 2008. Effect of a Bacillus-based direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growingfinishing pigs. J. Anim. Sci. 86:1459-1467. https://doi.org/10.2527/jas.2007-0603
  9. Domeneghini, C., Di Giancamillo, A., Arrighi, S. and Bosi, G. 2006. Gut-trophic feed additives and their effects upon the gut structure and intestinal metabolism. State of the art in the pig, and perspectives towards humans. Histol. Histopathol. 21:273-283.
  10. Fethiere, R. and Miles, R. D. 1987. Intestinal tract weight of chicks fed an antibiotics and probiotics. Nutr. Rep. Int. 36: 1305-1309.
  11. Francis, G., Kerem, Z., Makkar, H. P. S. and Becker, K. 2002. The biological action of saponins in animal systems: a review. Br. J. Nutr. 88:587-605. https://doi.org/10.1079/BJN2002725
  12. Hill, G. M., Mahan, D. C., Carter S. D., Gromwell, G. L., Ewan, R. C., Harrold, R. L., Lewis, A. J., Miller, P. S., Shurson, G. C. and Veum, T. L. 2001. Effect of pharmacological concentrations of zinc oxide with or without the inclusion of an antibacterial agent on nursery pig performance. J. Anim. Sci. 79:934-941. https://doi.org/10.2527/2001.794934x
  13. Ilsley, S. E., Millers, H. M. and Kamel, C. 2005. Effects of dietary quillaja saponin and curcumin on the performance and immune status of weaned piglets. J. Anim. Sci. 83:82-88. https://doi.org/10.2527/2005.83182x
  14. Jang, I. S., Ko, Y. H., Kang, S. Y. and Lee, C. Y. 2007. Effect of a commercial essential oil on growth performance, digestive enzyme activity and intestinal microflora population in broiler chickens. Anim. Feed Sci. Technol. 134:304-315. https://doi.org/10.1016/j.anifeedsci.2006.06.009
  15. Jang, I. S., Ko, Y. H. Yang, H. Y. Ha, J. S., Kim, J. Y., Kim, J. Y., Kang, S. Y., Yoo, D. H. Nam, D. S., Kim, D. H. and Lee, C. Y. 2004. Influence of essential oil components on growth performance and the functional activity of the pancreas and small intestine in broiler chickens.Asian-Aust. J. Anim. Sci. 17:394-400. https://doi.org/10.5713/ajas.2004.394
  16. Kang, P., Xiao, H. L., Hou, Y. Q., Ding, B. Y., Liu, Y. L., Zhu, H. L., Hu, Q. Z., Hu, Y. and Yin, Y. L. 2010. Effects of Astragalus polysaccharides, Achyranthes bidentata polysaccharides and Acantbepanax senticosus saponin on the performance and immunity in weaned pigs. Asian-Aust. J. Anim. Sci. 23, 750-756. https://doi.org/10.5713/ajas.2010.90526
  17. Kelly, D., Smyth, J. A. and McCracken, K. J. 1991. Digestive development of the early-weaned pig. 2. Effect of level of food intake on digestive enzyme activity during the immediate post-weaning period. Br. J. Nutr. 65:181-188. https://doi.org/10.1079/BJN19910079
  18. Kong, X. F., Wu, G. Y., Liao, Y. P., Hou, Z. P., Liu, H. J., Yin, F. G., Li, T. J., Huang, R. L., Zhang, Y. M., Deng, D., Kang, P., Wang, R. X., Tang, Z. Y., Yang, C. B., Deng, Z. Y., Xiong, H., Chu, W. Y., Ruan, Z., Xie, M. Y. and Yin, Y. L. 2007. Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance, serum metabolites and intestinal health in early-weaned piglets. Livest. Sci. 108: 272-275. https://doi.org/10.1016/j.livsci.2007.01.079
  19. Lalles, J. P., Bosi, P., Smidt, H. and Stokes, C. R. 2007. Nutritional management of gut health in pigs around weaning. Proc. Nutr. Soc. 66:260-268. https://doi.org/10.1017/S0029665107005484
  20. Lee, K. W. 2002. Essential oils in Broiler Nutrition. Thesis. Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
  21. Leser, T. D., Knarreborg, A. and Worm, J. 2008. Germination and outgrowth of Bacillus subtilis and Bacillus licheniformis spores in the gastrointestinal tract of pigs. J. Appl. Microbiol. 104:1025-1033. https://doi.org/10.1111/j.1365-2672.2007.03633.x
  22. MIFAFF. 2004. Classification and Specification of Hazardous Feeds (published in Korean; translated into English). Notification No. 2004-72, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.
  23. MIFAFF, 2010. Classification and Specification of Hazardous Feeds (published in Korean; translated into English). Notification No. 2010-30, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.
  24. Manzanilla, E. G., Nofrarías, M. Anguita, M., Castillo, M., Perez, J. F., Martin-Orue, S. M., Kamel, C. and Gasa. J. 2006. Effects of butyrate, avilamycin, and a plant extract combination on the intestinal equilibrium of early-weaned pigs. J. Anim. Sci. 84:2743-2751. https://doi.org/10.2527/jas.2005-509
  25. Miles, R. D., Butcher, G. D., Henry, P. R. and Littell, P. R. 2006. Effect of antibiotic growth promoters on broiler performance, intestinal growth parameters and quantitative morphology. Poult. Sci. 85:476-485. https://doi.org/10.1093/ps/85.3.476
  26. Ohashi, Y and Ushida, K. 2009. Health-beneficial effects of probiotics: its mode of action. Anim. Sci. J. 80:361-371. https://doi.org/10.1111/j.1740-0929.2009.00645.x
  27. Olas, B., Wachowicz, B., Stochmal, A. and Oleszek, W. 2003. Inhibition of oxidative stress in blood platelets by different phenolics from Yucca schidigera Roezl. bark. Nutrition 19:633-640. https://doi.org/10.1016/S0899-9007(03)00036-4
  28. Owusu-Asiedu, A., Nyachoti, C. M. and Marquardt, R. R. 2003. Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody, zinc oxide, fumaric acid, or antibiotic. J. Anim. Sci. 81:1790-1798. https://doi.org/10.2527/2003.8171790x
  29. Phillips, I. 2007. Withdrawal of growth-promoting antibiotics in Europe and its effects in relation to human health. Int. J. Antimicrob. Agents 30:101-107. https://doi.org/10.1016/j.ijantimicag.2007.02.018
  30. Ryniba, I. V., Liu, H., Gor, Y. and Feinmark, S. J. 1997. Regulation of leukotriene A4 hydrolase activity in endothelial cells by phosphorylation. J. Biol. Chem. 272:31865-31871. https://doi.org/10.1074/jbc.272.50.31865
  31. Sehm, J., Lindermayer, H., Dummer, C., Treutter, D. and Pfaffl, M. W. 2007. The influence of polyphenol rich apple pomace or red-wine pomace diet on the gut morphology in weaning piglets. J. Anim. Physiol. Anim. Nutr. 91:289-296. https://doi.org/10.1111/j.1439-0396.2006.00650.x
  32. Snary, E. L., Kelly, L. A., Davison, H. C., Teale, C. J. and Wooldridge, M. 2004. Antimicrobial resistance: a microbial risk assessment perspective. J. Antimicrob. Chemother. 53:906-917. https://doi.org/10.1093/jac/dkh182
  33. Solomon, S. E. and Tullett, S. G. 1988. The effect of virginiamycin on the ileum of the domestic fowl. 1. Light and scanning electron microscope observations. Anim. Tech. 39:157-160.
  34. Stein, H. H. and Kil, Y. 2006. Reduced use of antibiotic growth promoters in diets fed to weanling pigs: dietary tools, part 2. Anim. Biotechnol. 17:217-231. https://doi.org/10.1080/10495390600957191
  35. Stutz, M. W., Johnson, S. L. and Judith, F. R. 1983. Effects of diet, bacitracin, and body weight restrictions on the intestine of broiler chicks. Poult. Sci. 62:1626-1632. https://doi.org/10.3382/ps.0621626
  36. Thymann, T., Sorensen, K. U., Hedemann, M. S., Elnif, J., Jensen, B. B., Banga-Mboko, H., Leser, T. D. and Sangild, P. T. 2007. Antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure in weanling pigs. Br. J. Nutr. 97:1128-1137. https://doi.org/10.1017/S0007114507691910
  37. van der Peet-Schwering, C. M., Jansman, A. J., Smidt, H. and Yoon, I. 2007. Effects of yeast culture on performance, gut integrity, and blood cell composition of weanling pigs. J. Anim. Sci. 85:3099-3109. https://doi.org/10.2527/jas.2007-0110
  38. Veselinova, A., Ivanov, N. and Chotinski, D. 1987. Morphological changes in broilers produced by feeding antibiotics. Vet. Med. Nauki. 24:9-14.
  39. Visek W. J. 1978. The mode of growth promotion by antibiotics. J. Anim. Sci. 46:1447-1469. https://doi.org/10.2527/jas1978.4651447x
  40. Wenk, C. 2000. Recent advances in animal feed additives such as metabolic modifiers, antimicrobial agents, probiotics, enzymes and highly available minerals. Review. Asian-Aust. J. Anim. Sci. 13:86-95. https://doi.org/10.5713/ajas.2000.86
  41. Windisch, W. M., Schedle, K., Plitzner, C. and Kroismayr, A. 2008. Use of phytogenic products as feed additives for swine and poultry. J. Anim. Sci. 86(Suppl.):E140-E148. https://doi.org/10.2527/jas.2007-0459
  42. Yen, J. T. and Pond, W. G. 1993. Effects of carbadox, copper, or Yucca shidigera extract on growth performance and visceral weight of young pigs. J. Anim. Sci. 71:2140-2146. https://doi.org/10.2527/1993.7182140x
  43. Yin, F., Yin, Y., Kong, X., Liu, Y., He, Q., Li, T., Huang, R., Hou, Y., Shu, X., Tan, L., Chen, L., Gong, J., Kim, S. W. and Wu, G. 2008. Dietary supplementation with Acanthopanax senticosus extract modulates gut microflora in weaned piglets. Asian-Aust. J. Anim. Sci. 21:1330-1338. https://doi.org/10.5713/ajas.2008.70583
  44. Zhao, J., Harper, A. F., Estienne, M. J., Webb, Jr., K. E., McElroy, A. P. and Denbow, D. M. 2007. Growth performance and intestinal morphology responses in early weaned pigs to supplementation of antibiotic-free diets with an organic copper complex and spray-dried plasma protein in sanitary and nonsanitary environments. J. Anim. Sci. 85:1302-1310. https://doi.org/10.2527/jas.2006-434

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