Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

DNA Polymorphism in SLC11A1 Gene and its Association with Brucellosis Resistance in Indian Zebu (Bos indicus) and Crossbred (Bos indicus×Bos taurus) Cattle

  • Kumar, Nishant (Genome Analysis Laboratory, Animal Genetics Division, Indian Veterinary Research Institute, Indian Council of Agricultural Research) ;
  • Ganguly, Indrajit (Genome Analysis Laboratory, Animal Genetics Division, Indian Veterinary Research Institute, Indian Council of Agricultural Research) ;
  • Singh, Rajendra (Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Indian Council of Agricultural Research) ;
  • Deb, Sitangsu M. (Genome Analysis Laboratory, Animal Genetics Division, Indian Veterinary Research Institute, Indian Council of Agricultural Research) ;
  • Kumar, Subodh (Genome Analysis Laboratory, Animal Genetics Division, Indian Veterinary Research Institute, Indian Council of Agricultural Research) ;
  • Sharma, Arjava (Genome Analysis Laboratory, Animal Genetics Division, Indian Veterinary Research Institute, Indian Council of Agricultural Research) ;
  • Mitra, Abhijit (Genome Analysis Laboratory, Animal Genetics Division, Indian Veterinary Research Institute, Indian Council of Agricultural Research)
  • Received : 2010.08.24
  • Accepted : 2011.01.03
  • Published : 2011.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

The PCR- restriction fragment length polymorphism (RFLP) in and around TM4 of SLC11A1 gene and its association with the incidences of brucellosis in Hariana breed (Bos indicus) and Holstein Friesian crossbred (Bos indicus${\times}$Bos taurus) cattle was examined. A fragment of 954 bp encoding the TM4 was amplified, and RFLP was identified by digestion of the amplicon independently with AluI and TaqI. The amplicon (GenBank Acc. No. AY338470 and AY338471) comprised of a part of exon V (<59 bp) and VII (62>), and entire intron 5 (423 bp), exon VI (71 bp) and intron 6 (339 bp). Digestion with AluI revealed the presence of two alleles viz, A (281, 255, 79 and 51 bp) and B (541, 255, 79 and 51 bp). The frequency of A allele was estimated as 0.80 and 0.73 in Hariana and crossbred cattle, respectively. Due to presence of a polymorphic TaqI site at intron 5, two alleles: T (552 and 402 bp) and Q (231, 321 and 402 bp) were identified. The frequency of T allele was estimated as 0.96 and 0.97, respectively. For association study, on the basis of serological tests and history of abortion, the animals were grouped into "affected" and "non-affected". However, no association could be established with the observed RFLPs.

Keywords

References

  1. Abel, L., F. O. Sanchez, J. Oberti, N. V. Thuc, L. V. Hoa, V. L. Lap, E. Skamene, P. H. Lagrange and E. Schurr. 1998. Susceptibility to leprosy is linked to the human NRAMP1 gene. J. Infect. Dis. 177:133-145. https://doi.org/10.1086/513830
  2. Adams, L. G. and J. W. Templeton. 1998. Genetic resistance to bacterial diseases of animals. Rev. Sci. Tech. 17:200-219.
  3. Ahvonen, P., E. Jansson and K. Aho. 1969. Marked cross-agglutination between Brucellae and a subtype of Yersinia enterocolitica. Acta. Pathol. Microbiol. Scand. 75(2):291-295.
  4. Altet, L., O. Francino, L. Solano-Gallego, C. Renier and A. Sanchez. 2002. Mapping and sequencing of the canine NRAMP1 gene and identification of mutations in leishmaniasis-susceptible dogs. Infect. Immun. 70(6):2763-2771. https://doi.org/10.1128/IAI.70.6.2763-2771.2002
  5. Barthel, R., J. Feng, J. A. Piedrathia, D. N. McMurray, J. W. Templeton and L. G. Adams. 2001. Stable transfection of the bovine NRAMP1 gene into murine RAW264.7 cells: Effect on Brucella abortus survival. Infect. Immun. 69:3110- 3119. https://doi.org/10.1128/IAI.69.5.3110-3119.2001
  6. Bellamy, R., C. Rumende, T. Corrah, K. P. W. J. McAdam, H. C. Whittle and A. V. S. Hill. 1998. Variation in the human NRAMP1 gene and susceptibility to tuberculosis on Africans. New Engl. J. Med. 338:640-644. https://doi.org/10.1056/NEJM199803053381002
  7. Borriello, G., R. Capparelli, M. Bianco, D. Fenizia, F. Alfano, F. Capuano, D. Ercolini, A. Parisi, S. Roperto and D. Iannelli. 2006. Genetic resistance to Brucella abortus in the water buffalo (Bubalus bubalis). Infect. Immun. 74:2115-2120. https://doi.org/10.1128/IAI.74.4.2115-2120.2006
  8. Bradley, D. J., B. A. Taylor, J. Blackwell, E. P. Evans and J. Freeman, 1979. Regulation of Leishmania populations within the host III. Mapping of the locus controlling susceptibility to visceral leishmaniasis in the mouse. Clin. Exp. Immunol. 37:7-14.
  9. Campbell, G. A. and L. G. Adams. 1992. The long-term culture of bovine monocyte-derived macrophages and their use in the study of intracellular proliferation of Brucella abortus. Vet. Immunol. Immunopathol. 34(3-4):291-305. https://doi.org/10.1016/0165-2427(92)90171-L
  10. Capparelli, R., F. Alfano, M. G. Amoroso, G. Borriello, D. Fenizia, A. Bianco, S. Roperto, F. Roperto and D. Iannelli. 2007a. Protective effect of the Nramp1 BB genotype against Brucella abortus in water buffalo (Bubalus bubalis). Infect. Immun. 75:988-996. https://doi.org/10.1128/IAI.00948-06
  11. Capparelli, R., G. Borriello, R. Marabelli, S. Roperto, F. Roperto and D. Iannelli. 2007b. The Nramp1 AA genotype confers susceptibility to Brucella abortus in water buffalo. Mamm. Genome. 18:137-143. https://doi.org/10.1007/s00335-006-0103-x
  12. Corbell, M. J. 1975. The serological relationship between Brucella spp., Yersinia enterocolitica serotype IX and Salmonella serotypes of Kauffmann-White group N. J Hyg (Lond). 75(1):151-171. https://doi.org/10.1017/S0022172400047173
  13. Coussens, P. M., M. J. Coussens, B. C. Tooker and W. P. Nobis. 2004. Structure of the bovine natural resistance associated macrophage protein (NRAMP 1) gene and identification of a novel polymorphism DNA Seq. 15(1):15-25. https://doi.org/10.1080/10425170310001638945
  14. Crocker, P. R., J. M. Blackwell and D. J. Bradley. 1984. Expression of the natural resistance gene Lsh in resident liver macrophages. Infect. Immun. 43(3):1033- 1040.
  15. Denis, M., A. Forget, M. Pelletier and E. Skamene. 1988. Pleiotropic effects of the Bcg gene: III. Respiratory burst in Bcg-congenic macrophages. Clin. Exp. Immunol. 73:370-375.
  16. Ganguly, I., A. Sharma, A. Mitra, N. Kumar and A. Ganguly. 2011. Analysis of genetic variations of complete TM4 of buffalo (Bubalus bubalis) Slc11A1 gene. J. Appl. Anim. Res. (In Press)
  17. Ganguly, I., A. Sharma, R. Singh, S. M. Deb, D. K. Singh and A. Mitra. 2008. Association of microsatellite (GT)n polymorphism at 3'UTR of NRAMP1 with the macrophage function following challenge with Brucella LPS in buffalo (Bubalus bubalis). Vet. Microbiol. 129(1-2):188-196. https://doi.org/10.1016/j.vetmic.2007.10.033
  18. Gros, P., E. Skamene and A. Forget. 1981. Genetic control of natural resistance to Mycobacterium bovis (BCG) in mice. J. Immunol. 127(6):2417-2421.
  19. Guilloteau, L. A., J. Dornand, A. Gross, M. Olivier, F. Cortade, Y. L. Vern and D. Kerboeuf. 2003. Nramp1 is not a major determinant in the control of Brucella melitensis infection in mice. Infect. Immun. 71(2):621-628. https://doi.org/10.1128/IAI.71.2.621-628.2003
  20. Horin, P. and J. Matiasovic. 2000. Two polymorphic markers for the horse SLC11A1 (RNAMP1) gene. Anim. Genet. 31(2):152. https://doi.org/10.1046/j.1365-2052.2000.00599.x
  21. Horin, P., I. Rychlik, J. W. Templeton and L. G. Adams. 1999. A complex pattern of microsatellite polymorphism within the bovine NRAMP1 gene. Eur. J. Immunogenet. 26:311-313. https://doi.org/10.1046/j.1365-2370.1999.00148.x
  22. Jin, J., L. Sun, W. Jiao, S. Zhao, H. Li, X. Guan, A. Jiao, Z. Jiang and A. Shen. 2009. SLC11A1 (Formerly NRAMP1) gene polymorphisms associated with pediatric tuberculosis in China. Clin. Infect. Dis. 48(6):733-738. https://doi.org/10.1086/597034
  23. Kumar, G. S. N., M. G. Govindiah, C. S. Nagaraj, A. Bindu and T. J. Rasool. 1999. Does the Natural resistance associated macrophage protein (NRAMP1) gene confer resistance/susceptibility in bovine against mycobacterial infection? Curr. Sci. 77:809-812.
  24. Kumar, N., A. Mitra, I. Ganguly, R. Singh, S. M. Deb, S. K. Srivastava and A. Sharma. 2005. Lack of association of brucellosis resistance with $(GT)_{13}$ microsatellite allele at 3'UTR of NRAMP1 gene in Indian zebu (Bos indicus) and crossbred (Bos $indicus{\times}$Bos taurus) cattle. Vet. Microbiol. 111:139-143. https://doi.org/10.1016/j.vetmic.2005.09.012
  25. Lang, T., E. Prina, D. Sibthorpe and J. M. Blackwell. 1997. Nramp1 transfection transfers Ity/Lsh/Bcg-related pleiotropic effects on macrophage activation: influence on antigen processing and presentation. Infect. Immun. 65:380-386.
  26. Liu, J., T. M. Fujiwara, N. T. Buu, F. O. Sanchez, M. Cellier, A. J. Paradis, D. Frappier, E. Skamene, P. Gros, K. Morgan and E. Schurr. 1995. Identification of polymorphisms and sequence variants in the human homologue of the mouse natural resistance associated macrophage protein gene. Am. J. Hum. Genet. 56:845-853.
  27. Malo, D., K. Vogan, S. Vidal, J. Hu, M. Cellier, E. Schurr, A. Fuks, N. Bumstead, K. Morgan and P. Gros. 1994. Haplotype mapping and sequence analysis of the mouse Nramp1 gene predict susceptibility to infection with intracellular parasites. Genomics 23:51-61. https://doi.org/10.1006/geno.1994.1458
  28. Marquet, S., P. Lepage, T. J. Hudson, J. M. Musser and E. Schurr. 2000. Complete nucleotide sequence and genomic structure of the human NRAMP1 gene region on chromosome region 2q35. Mamm. Genome 11(9):755-762.
  29. Martinez, R., R. Toro. F. Montoya, M. Burbano, J. Tobon, J. Gallego, S. Dunner and J. Canon. 2008. Bovine SLC11A1 3' UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain. Anim. Breed. Genet. 125(4):271-279. https://doi.org/10.1111/j.1439-0388.2008.00727.x
  30. Matthews, G. D. and A. M. Crawford. 1998. Cloning, sequencing and linkage mapping of the NRAMP1 gene of sheep and deer. Anim. Genet. 29:1-6. https://doi.org/10.1046/j.1365-2052.1998.00224.x
  31. Nielsen, K.H., D. Gall, W. Kelly, D. Henning and M. M. Gracia. 1992. Enzyme Immunoassay: Application to Diagnosis of Bovine Brucellosis. Monograph, Agriculture Canada, Nepean, Ont.
  32. Paixao, T. A., F. P. Poester, N. A. V. Carvalho, A. M. Borges, A. P. Lage and R. L. Santos. 2007. NRAMP1 3'Untranslated region polymorphisms are not associated with natural resistance to Brucella abortus in cattle. Infect. Immun. 75:2493-2499. https://doi.org/10.1128/IAI.01855-06
  33. Plant, J. and A. A. Glynn. 1976. Genetics of resistance to infection with Salmonella typhimurium in mice. J. Infect. Dis. 133:72-78. https://doi.org/10.1093/infdis/133.1.72
  34. Price, R. E., J. W. Templeton, R. Smith III and L.G. Adams. 1990. Ability of mononuclear phagocytes from cattle naturally resistant or susceptible to brucellosis to control in vitro intracellular survival of Brucella abortus. Infect. Immun. 58:879-886.
  35. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual. Vol 3. Cold Spring Harbour Library Press. pp. 6.3-6.4.
  36. Sandulache, R. and A. Marx. 1978. Immunochemical studies on a Yersinia enterocolitica O:9 lipopolysaccharide cross-reacting with Brucella abortus and Vibrio cholerae extracts. Ann. Microbiol (Paris). 129 B(3):425-435.
  37. Seabury, C. M., N. D. Halbert, P. J. P. Gogan, J. W. Templeton and J. N. Derr. 2005. Bison PRNP genotyping and potential association with Brucella spp. Seroprevalance. Anim. Genet. 36:104-110. https://doi.org/10.1111/j.1365-2052.2005.01240.x
  38. Templeton, J. W., R. Smith III and L. G. Adams. 1988. Natural disease resistance in domestic animals. J. Am. Vet. Med. Assoc. 192:1306-1315.
  39. Vidal, S. M., E. Pinner, P. Lepage, S. Gauthier and P. Gras. 1996. NRAMP1 encodes a membrane phosphoglycoprotein absent in macrophages from susceptible (NRAMP1D169) mouse strain. J. Immunol. 157:3559-3568.
  40. Wu, Z. F., W. H. Luo, G. F. Yang and X. Q. Zhang. 2007. Genomic organization and polymorphisms detected by denaturing high-performance liquid chromatography of porcine SLC11A1 gene. DNA Seq. 18(5):327-333. https://doi.org/10.1080/10425170600857541
  41. Zhang, G., H. Wu, C. R. Ross, J. E. Minton and F. Blecha. 2000. Cloning of porcine NRAMP1 and its induction by lipopolysaccharide, tumor necrosis factor alpha, and interleukin-1beta: role of CD14 and mitogen- activated protein kinases. Infect. Immun. 68(3):1086-1093. https://doi.org/10.1128/IAI.68.3.1086-1093.2000

Cited by

  1. gene vol.39, pp.4, 2011, https://doi.org/10.1080/09712119.2011.607943
  2. Polymorphism of cytokine and innate immunity genes associated with bovine brucellosis in cattle vol.41, pp.5, 2014, https://doi.org/10.1007/s11033-014-3136-3
  3. Polymorphisms of TLR1, TLR4 and SLC11A1 Genes in Some Cattle Breeds Reared in Turkey vol.24, pp.4, 2011, https://doi.org/10.15832/ankutbd.349728
  4. Single-nucleotide polymorphisms in CLEC7A, CD209 and TLR4 gene and their association with susceptibility to paratuberculosis in Indian cattle vol.99, pp.1, 2011, https://doi.org/10.1007/s12041-019-1172-4