Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative effects of hesperidin and hesperetin under cellular system

Hesperidin과 hesperetin의 cellular system에서의 항산화 효과

  • Cho, Eun-Ju (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Li, Li (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Yamabe, Noriko (Institute of Natural Medicine, University of Toyama) ;
  • Kim, Hyun-Young (Department of Food Science, Gyeongnam National University of science and technology)
  • 조은주 (부산대학교 식품영양학과 및 노인생활환경연구소) ;
  • 이여 (부산대학교 식품영양학과 및 노인생활환경연구소) ;
  • ;
  • 김현영 (경남과학기술대학교 식품과학부)
  • Received : 2011.11.03
  • Accepted : 2011.12.18
  • Published : 2011.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the antioxidant activity of hesperidin and hesperetin, which are the active compounds from Citrus junos, in the cellular system. Under cellular model of oxidative damage using LLC-$PK_1$ renal epithelial cell, the oxidative damage induced by 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) led to the loss of cell viability, while treatment of hesperidin and hesperetin increased significantly the cell viability as dose-dependent manner. In addition, NO-induced cellular oxidative damage by sodium nitroprusside were significantly recovered by the treatment of hesperidin and hesperetin, showing the increase of cell viability. But hesperidin and hesperetin showed no significant protective effect on $O_2{^-}$-induced cellular oxidative damage. The present study indicates that hesperidin and hesperetin protect against free radical, especially AAPH-induced peroxyl radical. In particular, hesperetin has stronger protective effect against oxidative stress than hesperidin.

Keywords

References

  1. Ahn MS, Kim HJ, Seo MS. 2007. A study on the antioxidative and antimicrobial activites of the citrus Unshiju peel extracts. Korean J. Food Culture 22: 454-461.
  2. Altman SA, Zastawny TH, Randers L, Lin Z, Lumpkin JA, Remacle J, Dizdaroglu M, Rao G. 1994. Tert-butyl hydroperoxide-mediated DNA base damage in cultured mediated DNA base damage in cultured mammalian cells. Mutat. Res. 306: 35-44. https://doi.org/10.1016/0027-5107(94)90165-1
  3. Andreoli SP, McAteer JA, Seifert SA, Kempson SA. 1993. Oxidant-induced alterations in glucose and phosphate transport in LLC-PK1 cells: mechanisms of injury. Am. J. Physiol. 265: 377-384.
  4. Bauer G. 2000. Reactive oxygen and nitrogen species: efficient, selective and interactive signals during intercellular induction of apoptosis. Anticancer Res. 20: 4115-4139.
  5. Brune B, Zhou J, Von KA. 2003. Nitric oxide, oxidative stress and apoptosis. Kidney Int. Suppl. 84: 22-24.
  6. Calomme M, Pieters L, Vlietinck A, Berghe DV. 1996. Inhibition of bacterial mutagenesis by citrus flavonoids. Planta Med. 62: 222-226. https://doi.org/10.1055/s-2006-957864
  7. Cha JY, Kim SY, Jeong SJ, Cho YS. 1999. Effects of hesperidin and naringenin on lipid concentration in orotic acid treated mices. Korean J. Life Science 9: 389-394.
  8. Choi EJ. 2007. Hesperetin induced G1-phase cell cycle arrest in human breast cancer MCF-7 cells: involvement of CDK4 and p21. Nutrition and cancer 59: 115-119. https://doi.org/10.1080/01635580701419030
  9. Garg A, Garg S, Zaneveld LJD, Singla AK. 2001. Chemistry and pharmacology of the citrus bioflavonoid hesperidin. Phytother Res. 15: 655-669. https://doi.org/10.1002/ptr.1074
  10. Huong DTL, Dat NT, Cai XF, Shen GH, Bae KH, Kim YH. 2004. Phenolic components from the leaves and twigs of Rhamnus taquetii. Korean J. Pharmacogn. 35: 139-142.
  11. Kawaguchi K, Mizuno T, Aida K, Uchino K. 1997. Hesperidin as an inhibotor of lipases from porcine pancreas and pseudomonas. Biosci. Biotechnol. Biochem. 61: 102-104. https://doi.org/10.1271/bbb.61.102
  12. Kim J, Kim SA, Yun WK, Kim EJ, Woo MK, Lee MS. 2004. Antioxidative effect of ethanol extract for 5 kinds of spice. J. Korean Soc. Food Sci. Nutr. 33: 524-528.
  13. Kobayashi S, Tanabe S. 2006. Evaluation of the anti-allergic activity of Citrus unshiu using rat basophilic leukemia RBL-2H3 cells as well as basophils of patients with seasonal allergic rhinitis to pollen. Int. J. Mol. Med. 17: 511-515.
  14. Middleton EJ, Kandaswami C, Theoharides TC. 2000. The effects of plant flavonoids on mammalian cells: implications for inflammation, hearts disease, cancer. Pharmacol Rev. 52: 673-751.
  15. Monforte MT, Trovato A, Kirjavainen S, Forestieri AM, Galati EM. 1995. Biological effects of hesperidin, a citrus flavonoid. (note II): Hypolipidemic activity on experimental hypercholesterolemia in rat. Farmco. 50: 595-599.
  16. Mosmann T. 1983. Rapid colormetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth. 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  17. Niki E. 1990. Free radical initiators as source of water-or lipid-soluble peroxyl radicals. Meth. Enzymol. 186: 100-108.
  18. Nunokawa Y, Tanaka S. 1992. Interferon-γ inhibits proliferation of rat vascular smooth muscle cells by nitric oxide generation. Biochem. Biophys. Res. Commun. 188: 409-415. https://doi.org/10.1016/0006-291X(92)92400-R
  19. Oberley TD, Schultz JL, Li N, Oberley LW. 1995. Antioxidant enzyme levels as a function of growth state in cell culture. Free Radic. Biol. Med. 19: 53-65. https://doi.org/10.1016/0891-5849(95)00012-M
  20. Rice-Evans C, Burdon R. 1993. Free radical-lipid interaction and their pathological consequences. Prog. Lipid Res. 32: 71-110. https://doi.org/10.1016/0163-7827(93)90006-I
  21. Schneider JE, Price S, Maidt L, Gutteridge JM, Floyd RA. 1990. Methylene blue plus light mediates 8-hydroxy-2'- deoxy-guanosine formation in DNA preferentially over strand breakage. Nucleic Acids Res. 18: 631-635. https://doi.org/10.1093/nar/18.3.631
  22. So FV, Guthrie N, Chambers AF, Moussa M, Carroll KK. 1996. Inhibition of human breast cancer cell proliferation and delay of mammary tumorigenesis by flavonoids and citrus juices. Nutr. Cancer 26: 167-181. https://doi.org/10.1080/01635589609514473
  23. Sohn JS, Kim MK. 1998. Effects of hesperidin and naringin on antioxidative capacity in the rat. Korean Nutr. Soc. 31: 687-696.
  24. Stratton SP, Liebler DC. 1997. Determination of singlet oxygen-specific versus radical-mediated lipid peroxidation in photosensitized oxidation of lipid bilayers: effect of betacarotene and alpha-tocopherol. Biochemistry 36: 12911-12920. https://doi.org/10.1021/bi9708646
  25. Terao K, Niki E. 1986. Damage to biological tissue induced by radical initiator 2,2'-azobis (2-amidinopropane) dihydrochloride and its inhibition by chain-breaking antioxidants. Free Radic. Biol. Med. 2: 193-201. https://doi.org/10.1016/S0748-5514(86)80070-8
  26. Woo KL, Kim JL, Kim MC, Chang DK. 2006. Determination of flavonoid and limonoid compounds in citron (Citrus junos SIEB ex TANAKA) seeds by HPLC and HPLC/MS. J. Korean Soc. Food Sci. Nutr. 35: 353-358. https://doi.org/10.3746/jkfn.2006.35.3.353
  27. Wright AW, Bubb A, Hawkins CL, Davies MJ. 2002. Singlet oxygen-mediated protein oxidation: evidence for the formation of reactive side chain peroxides on tyrosine residues. Photochem. Photobilo. 76: 35-46. https://doi.org/10.1562/0031-8655(2002)076<0035:SOMPOE>2.0.CO;2
  28. Yu HE, Leaniza MM, Bae YJ, Lee DH, Park JS, Kwak SH, Kim HK, Lee JS. 2005. Screening and extration condition of antiaging bioactive substances from medicinal plants. J. Korean Soc. Food Sci. Nutr. 34: 1136-1142. https://doi.org/10.3746/jkfn.2005.34.8.1136