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한우 FASN 유전자변이와 한우 지방형질 및 지방산조성과의 연관성 분석

Association of genetic polymorphism in fatty acid synthase with BodyFat and fatty acid composition in Hanwoo

  • 이승환 (농촌진흥청 국립축산과학원 한우시험장) ;
  • 김형철 (농촌진흥청 국립축산과학원 한우시험장) ;
  • 조원모 (농촌진흥청 국립축산과학원 한우시험장) ;
  • 장선식 (농촌진흥청 국립축산과학원 한우시험장) ;
  • 김범수 (농촌진흥청 국립축산과학과 동물유전체과) ;
  • 장길원 (농촌진흥청 국립축산과학과 동물유전체과) ;
  • 이준헌 (충남대학교 동물자원생명과학과) ;
  • 연성흠 (농촌진흥청 국립축산과학과 동물유전체과) ;
  • 홍성구 (농촌진흥청 국립축산과학원 한우시험장)
  • Lee, Seung-Hwan (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Hyeong-Cheol (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, Won-Mo (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Chang, Sun-Sik (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Bum-Soo (Animal Genome& Bioinformatics Division, NIAS, RDA) ;
  • Chang, Gul-Won (Animal Genome& Bioinformatics Division, NIAS, RDA) ;
  • Lee, Jun-Hun (Department of Animal Science & Biotechnology, Chungnam National University) ;
  • Yeon, Seong-Heum (Animal Genome& Bioinformatics Division, NIAS, RDA) ;
  • Hong, Seong-Koo (Hanwoo Experiment Station, National Institute of Animal Science, RDA)
  • 투고 : 2011.04.18
  • 심사 : 2011.06.20
  • 발행 : 2011.06.30

초록

The aim of this study was to identify the polymorphism on fatty acid synthase (FASN) gene and its association with fat traits and fatty acid composition in Hanwoo. We genotyped a SNP (g.16024G${\rightarrow}$A) detected in Exon34 of FASN on 90 Hanwoo steers by PCR RFLP. A linear mixed model revealed an association of g.16024G${\rightarrow}$A with total body fat contents (P=0.006), while there is no significant effect between g.16024G${\rightarrow}$A and other traits (IMF, BF and fatty acid composition). Regression coefficient and $R^2$ of the SNP was -1.5 kg and 0.36 in this anslysis. Expecially, AA type of g.16024G${\rightarrow}$A bas a less amount of body fat (1.5 kg) than GG type of the SNP in Hanwoo. In conclusion, this study indicates an important role for FASN gene in determining body fat content in Hanwoo.

키워드

참고문헌

  1. Berndt J, Kovacs P, Ruschke K, Kloting N, Fasshauer M, Schon M, Stumvoll M, Bluher M. 2007. Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes. Diabetologia. 50: 1472-1480. https://doi.org/10.1007/s00125-007-0689-x
  2. Childs K, Goad W, Allan F, Pomp D, Krehbiel C, Geisert D, Morgan B, Malayer R. 2002. Differential expression of NAT1 translational repressor during development of bovine intramuscular adipocytes. Physiol. Genomics. 10: 49-56.
  3. Geay Y, Bauchart D, Hocquette J, Culioli J. 2001. Effect of nutritional factors on biochemical, structural and metabolic factors on of muscles in ruminant, consequences on dietic value and sensorial qualities of meat. Reprod. Nutr. Dev. 41: 1-26. https://doi.org/10.1051/rnd:2001108
  4. Gilmour A, Gogel J, Cullis R, Thompson R. 2006. ASREML User Guide Release 2.0 VSN International Ltd., Hemel Hempstead, HP1 1ES, UK.
  5. Hayes B. 2008. QTL mapping, MAS and Genomic selection. Text book for Armidale summer animal breeding course.
  6. Hocquette J, Richardson I, Prachardson I, Prache S, Medale F, Duffy G, Scollan, N. 2005. The future trends for research on quality and safety of animal product. Italian J. Anim. Sci. 4: 49-72.
  7. Kim KH, Lee JH, Oh YG, Kang SW, Lee SC, Park WY, Ko YD. 2005.The Optimal TDN Levels of Concentrates and Slaughter Age in Hanwoo Steers. J Anim Sci & Technol (Kor.). 47: 731-744. https://doi.org/10.5187/JAST.2005.47.5.731
  8. Lee SH, Yoon D, Choi NJ, Hwang SH, Cheong EY, Oh SJ, Cheong LC, Lee CS. 2004. Developmental Relationship of Unsaturated fatty acid composition and stearoyl- coA desaturase mRNA level in Hanwoo steers muscle. Asian- Aust. J. Anim. Sci. 18: 562-566.
  9. Lee SH, Park EW, Cho YM et al. 2006. Lipogenesis gene expression profiling on the early and late fattening stage of Hanwoo muscle. J. Anim. Sci & Technol(kor). 48: 913-920.
  10. Liu H, Liu JY, Zhang JT. 2010. Biochemistry, molecular biology, and pharmacology of fatty acid synthase, an emerging therapeutic target and diagnosis/prognosis marker. Int. J. Biochem. Mol. Bio. 1(1): 69-89.
  11. Lin CY, Smith S. 1978. Properties of the thioesterase component obtained by limited trypsinization of the fatty acid synthetase multienzyme complex. J. Biol. Chem. 253: 1954-62.
  12. Morris CA, Cullen NG, Glass BG, Hyndman DL, Manley TR, Hickey SM, McEwan JC, Pitchford WS, Bottema CDK, Lee MAH. 2007. Fatty acid synthase effects on bovine adipose fat and milk fat. Mamm. Genome. 18: 64-74.
  13. Pazirandeh M, Chirala S, Huang Y, Wakil J. 1989. Characterization of recombinant thioesterase and acyl carrier protein domains of chicken fatty acid synthase expressed in Escherichia coli. J. Biol. Chem. 30: 18195-18201.
  14. Roy R, Ordovas L, Zaragoza P, Romero A, Moreno C, Altarriba J, Rodellar C. 2006. Association of polymorphisms in the bovine FASN gene with milk fat content. Anim. Genet. 37: 215-218. https://doi.org/10.1111/j.1365-2052.2006.01434.x
  15. Smith S. 1994. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB. J. 8: 1248-1259.
  16. Tatum J, Smith C, Carpenter Z. 1982. Interrelationships between marbling, subcutaneous fat thickness, and cooked beef palatability. J. Anim. Sci. 43: 777-784.
  17. Wakil S, Stoops K, Joshi C. 1983. Fatty acid synthesis and it's regulation. A. R. Biochem. 52: 537-579. https://doi.org/10.1146/annurev.bi.52.070183.002541
  18. Wakil S. 1989. Fatty acid synthase, a proficient multifunctional enzyme, Biochem. 28: 4523-4530. https://doi.org/10.1021/bi00437a001
  19. Wang YH, Byrne K, Reverter A, Harper G, Taniguchi M, McWilliam S, Mannen H, Oyama K, Lehnert S. 2005. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mamm. Genome 16: 201-210. https://doi.org/10.1007/s00335-004-2419-8
  20. Zhang S, Knight T, Reecy J, Beitz D. 2008. DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition. Anim. Genet. 39: 62-70. https://doi.org/10.1111/j.1365-2052.2007.01681.x