농업과학연구 (Korean Journal of Agricultural Science)

  • 제38권1호
  • /
  • Pages.11-16
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  • 2011
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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잣나무(Pinus koraiensis)의 cDNA library 제작 및 EST 분석

Construction of a full-length cDNA library from Pinus koraiensis and analysis of EST dataset

  • Kim, Joon-Ki (Department of Horticulture, Chungnam National University) ;
  • Im, Su-Bin (Department of Horticulture, Chungnam National University) ;
  • Choi, Sun-Hee (Department of Horticulture, Chungnam National University) ;
  • Lee, Jong-Suk (Department of Horticulture, Chungnam National University) ;
  • Roh, Mark S. (USDA, Agricultural Research Service, National Arboretum, Floral and Nursery Plants Research Unit) ;
  • Lim, Yong-Pyo (Department of Horticulture, Chungnam National University)
  • 투고 : 2011.01.11
  • 심사 : 2011.03.09
  • 발행 : 2011.03.30
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초록

In this study, we report the generation and analysis of a total of 1,211 expressed sequence tags (ESTs) from Pinus koraiensis. A cDNA library was generated from the young leaf tissue and a total of 1,211 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. In all, 857 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 50 nucleotides. A total of 411 unigene, consisting of 89 contigs and 322 singletons, was identified after assembling. Also, we identified 77 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 63.1% of ESTs were homologous with known function and 22.2% of ESTs were matched with putative or unknown function. The remaining 14.6% of ESTs showed no significant similarity to any protein sequences found in the public database. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Pinus and provided for the useful tools as a genetic resource.

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참고문헌

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피인용 문헌

  1. Investigation of Active Anti-Inflammatory Constituents of Essential Oil from Pinus koraiensis (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells vol.11, pp.6, 2021, https://doi.org/10.3390/biom11060817