Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Analysis of Fe-Deficient Inducing Enzyme and Required Time for Recovery of Nutritional Disorder by Fe-DTPA Treatment in the Fe-Deficient Induced Tomato Cultivars

토마토 품종별 철 결핍 유도후 Fe-DTPA 처리에 의한 영양장애 회복 소요시간과 철 결핍 유발물질 동정

  • Lee, Seong-Tae (Gyeongsangnamdo Agricultural Research and Extension Services) ;
  • Kim, Min-Keun (Gyeongsangnamdo Agricultural Research and Extension Services) ;
  • Lee, Young-Han (Gyeongsangnamdo Agricultural Research and Extension Services) ;
  • Kim, Young-Shik (Department of Plant Science and Technology, Sangmyung Univ.) ;
  • Kim, Yeong-Bong (Gyeongsangnamdo Agricultural Research and Extension Services)
  • Received : 2011.08.30
  • Accepted : 2011.10.07
  • Published : 2011.10.31
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Abstract

The purpose of this study was to find out required time for recovery of nutritional disorder by Fe-DTPA treatment in induced Fe-deficient tomato cultivars and to select stable Fe-chelate in high pH of nutrient solution. The pH levels of nutrient solution were amended with 6.0, 7.0, and 8.0. Then Fe-EDTA (Ethylenediaminetetraacetic acid, ferric-sodium salt), Fe-DTPA (Sodium ferric diethylenetriamine pentaacetate), and Fe-EDDHA (Ethylenediamine-N,N-bis (2-hydroxyphenylacetic acid) ferric-sodium salt)) were treated as Fe $2.0mg\;L^{-1}$ concentration. The Fe-DTPA and Fe-EDDHA were stable in the nutrient solution of pH 6.0~8.0 but Fe-EDTA in nutrient solution of pH 8.0 was to become insoluble by 25%. The Fe $2.0mg\;L^{-1}$ as Fe-DTPA was treated for recovery of Fe deficient tomato seedlings. In case of Redyoyo and Supersunroad cultivars, total chlorophyll and Fe contents of leaves were recovered as much as those of normal leaves in 5 days. The Rafito cultivar for complete recovery was taken 7 days. When Fe $2.0mg\;L^{-1}$ as Fe-DTPA was supplied to Fe-deficient tomato seedlings, in geotype, heme oxigenase recovered as much as normal leaves in 24 hours in the Rafito and Redyoyo. However, it was not remarkable difference by elapsed time in the Supersunroad.

토마토 수경재배시 양액의 pH가 높은 조건에서도 철이 불용화 되지 않고 pH에 안정적인 킬레이트 철의 선택, 3종의 토마토 묘종 (라피토, 레드요요, 수퍼선로드)에서 엽의 철 결핍 증상시 Fe-DTPA 철 공급에 의한 철 결핍 회복 소요시간과 철 결핍 유발물질 유전자를 구조 분석한 결과는 다음과 같다. 양액의 pH가 6.0, 7.0 및 8.0 수준에서 양액 중 철의 농도가 $2.0mg\;L^{-1}$로 되게 각각 3종류 (Fe-EDTA, Fe-DTPA, Fe-EDDHA)의 킬레이트 철을 처리한 결과 Fe-DTPA와 Fe-EDDHA 형태의 철은 불용화가 거의 없었지만 Fe-EDTA 는 양액의 pH가 7.0 일 때 철 함량은 $1.72mg\;L^{-1}$, 8.0일 때 철 함량은 $1.51mg\;L^{-1}$로 25% 정도 불용화가 일어났다. 철 결핍이 발생된 3종의 토마토 묘종에 Fe-DTPA 킬레이트 철 $2.0mg\;L^{-1}$ 농도의 양액을 공급하였을 때 토마토 엽의 엽록소와 철 함량이 정상으로 회복되는데 소요시간은 라피토 품종은 7일, 레드요요와 수퍼선로드 품종은 5일 소요되었다. 유전적 수준에서 철의 생합성에 관여하는 heme oxigenase의 발현은 Fe-DTPA $2.0mg\;L^{-1}$ 처리 24시간 이내에 라피토와 레드요요는 정상적인 수준으로 회복되었으나 수퍼선로드는 처리시간의 경과에 따라서 현저한 차이가 없었다.

Keywords

References

  1. Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts, polyphenol oxidase in Beta vulgaris. Plant Physiol. 24:1-5. https://doi.org/10.1104/pp.24.1.1
  2. Choi, J.M., S.K. Jeong, and K.D. Ko. 2009. Characterization of symptom and determination of tissue critical concentration for diagnostic criteria in 'aehyang' Strawberry as influenced by phosphorus concentrations in the fertigation solution. Kor. J. Hort. Sci. Technol. 27(1):55-61.
  3. Choi, J.M., S.K. Jeong, K.H. Cha, H.J. Chung, and K.S. Seo. 2000. Deficiency symptom, growth characteristics, and nutrient uptake of 'Nyoho' strawberry as affected by controlled potassium concentrations in fertilizer solution. J. Kor. Soc. Hort. Sci. 41:350-355.
  4. Chung, J.B., B.J. Kim, K.S. Ryu, S.H. Lee, H.J. Shin, T.K. Hwang, H.Y. Choi, Y.W. Lee, Y.J. Lee, and J.J. Kim. 2006 Relationships between micronutrient contents in soils and crops of plastic film house. Korean J. Environ. Agric. 25:217-227. https://doi.org/10.5338/KJEA.2006.25.3.217
  5. El-Fouly, M.M., O.A. Nofal, and Z.M. Mobarak. 2001. Effect of soil treatment with iron, manganese and zinc on growth and micronutrient uptake of sunflower plant grown in high-pH soil. J. Agron. Crop Sci. 186, 245-251. https://doi.org/10.1046/j.1439-037x.2001.00479.x
  6. Fox, C.D., R.L. Chaney, and M.C. White. 1978. The physiology of metal toxicity in plants. Annu. Rev. Plant Physiol. 29:511-567. https://doi.org/10.1146/annurev.pp.29.060178.002455
  7. Graham, R.D. and M.J. Webb. 1991. Micronutrients and disease resistance and tolerance in plant, p. 329-370. In Mortvedt, J.J. et al. (ed.) Micronutrient in agriculture, 2nd ed. SSSA book series No. 4, Madison, WI, USA.
  8. Hanson, E. 1993. Apples and pears, p. 159-163. In W. F. Bennett(ed.). Nutrient deficiencies and toxicities in crop plants. Amer. Phytopathol. Soc., St. Paul, MN, USA.
  9. Hirschi, K.D. 1999. Expression of Arabidobsis CAX1 in tobacco: Altered calcium homeostasis and dicreased sterss sensitivity. Plant Cell 11:2113-2122. https://doi.org/10.1105/tpc.11.11.2113
  10. Korcak, R.F. 1987. Iron deficiency chlorosis. Hort. Rev. 9:133-186.
  11. Marschner, H. 1995. Mineral nutrition of higher plant, 2nd ed. Academic Press INC., London, UK.
  12. Ministry of Environment. 2008. Standard methods of water sampling and analysis. Ministry of Environment, Incheon, Korea.
  13. Osawa T. and H. Ikeda. 1976. Heavy metal toxcicities in vegetable crops. I. The effect of iron concentrations in the nutrient solution manganase toxcicities in vegetable crops. J. Japan Soc. Hort. Sci. 45:50-58. https://doi.org/10.2503/jjshs.45.50
  14. Shin, P.G., S.C. Hong, A.C. Chang, J.Y. Lee, B.C. Jang, and K.S. Lee. 2004. Development of diagnostic method accurately for nutritional disorder in vegetable crops. Agricultural Environment Research, 385-393.
  15. Welch, R.M., W.H. Allway, W.A. House, and J. Kubota. 1991. Geographic distribution of trace elements, p. 31-57. In Mortvedt, J.J. et al. (ed.) Micronutrient in agriculture, 2nd ed. SSSA book series No. 4, Madison, WI, USA.
  16. Yun, S.K., J.K. Kim, S.J. Kim, and H.J. Lee. 2003. Causes for deficiency chlorosis in oriental pear (Pyrus pyrifolia) trees. J. Kor. Soc. Hort. Sci. 44:215-219.
  17. Zekri, M. and T.A. Obreza. 2009. Micronutrient deficiencies in citrus: iron, zinc, and manganese. Institute of Food and Agricultural Sciences, University of Florida. SL 204:1-3.

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