DOI QR코드

DOI QR Code

Antimicrobial Activity and Antioxidant Effect of Curcuma longa, Curcuma aromatica and Curcuma zedoaria

국내산 울금, 강황 및 보라울금의 항균활성과 항산화 효과

  • Kim, Hyun-Jin (Department of Food Science and Technology, Sunchon National University) ;
  • Lee, Jung-Won (Department of Food Science and Technology, Sunchon National University) ;
  • Kim, Yong-Doo (Department of Food Science and Technology, Sunchon National University)
  • Received : 2010.10.07
  • Accepted : 2011.02.11
  • Published : 2011.04.30

Abstract

We investigated the antimicrobial and antioxidant activities. and total polyphenol contents of Curcuma longa. Curcuma aromatica. and Curcuma zedoaria. In antimicrobial activity assays of solvent extracts, ethanol extract showed the highest activity compared with other extracts. The antimicrobial activity of ethanol extract of C. longa showed higher than those of C. aromatica, and C. zedoaria. Antimicrobial substance in ethanol extract of C. longa was maintained after heating at $100^{\circ}C$ for 30 min and not affected by changes in pH. DPPH free radical scavenging activity of solvent extracts were high in the order of ethanol, ethylacetate, ether, water, and hexane fractions, C. longa showed greater DPPH free radical scavenging activity than those of the C. aromatica, and C. zedoaria. Total polyphenol content of C. longa and C. aromatica exhibited were similar levels, whereas C. zedoaria was slightly lower concentration.

본 연구에서는 울금의 품종별 생리활성을 확인하기 위하여 우리나라에서 재배되고 있는 울금, 강황 및 보라울금의 근경을 실험재료로 하여 total polyphenol 함량, 항균활성, 항균물질의 열안정성 및 pH안정성과 항산화활성을 측정하였다. 울금 품종별 total polyphenol 함량은 울금과 강황에서 보라울금에 비하여 높은 함량을 나타내었다. 울금의 추출 용매에 따른 항균활성은 ethanol 추출물이 15.4~16.3 mm로 가장 강한 항균활성을 나타내었으며 ethylacetate, ether, hexane 순으로 높은 활성을 나타내었다. 품종별로는 울금 ethanol 추출물의 항균활성이 다른 품종에 비하여 높게 나타났으나 E coli와 젖산균 2종, 효모 2종에서는 항균활성이 나타나지 않았다. 울금 ethanol 추출물은 열 및 pH변화에 안정함을 확인하였다. 추출물별 울금의 전자공여능은 ethanol 추출물에서 74.2%로 높은 항산화성을 보였고 ethyl acetate, ether, water, hexane 추출물 순으로 나타났다. 품종별 울금의 전자공여능을 ethanol 추출물에서 비교했을 때 울금 74.2%, 강황 43.98%, 보라울금 43.05%로 나타나 울금에서 강한 항산화성을 보였다. 대조구로 사용한 vitamin C와 BHA는 각각 95%와 96.1%의 높은 전자공여능을 나타내었다. 울금 품종별 항산화성은 울금이 강황과 보라울금에 비하여 월등히 높음을 확인하였다.

Keywords

References

  1. KFDA (1998) Korean Herbal Pharmacopoeia. 2ed Korean Medical Index, Seoul, Korea, P 69-70, 468-470
  2. Ahn DG (2000) Korean herbal flora. Kyohak Publishing Co, Seoul, Korea, p 568-569
  3. Choi SK (2003) Effect of shade-method on agronomic characteristics of Curcuma aromatica Salisbury in southern island of korea. Korean J Plant Res, 16, 207-211
  4. Kang WS, Kim SH, Park EJ, Yoon KR (1998) Antioxidative property of turmeric (Curcumae Rhizoma) ethanol extract. Korean J Food Sci Technol, 30, 266-271
  5. Kim CR (2006) Enhancement of liver function by Curcuma extract on acute hepatotoxicity in rat. Korean J Food Sci Ani Resour, 26, 386-393
  6. Chi HJ, Kim HS (1983) Curcumin content of cultivated tumeric in Korea. Kor J Pharmacogn, 14, 67-69
  7. Jeong SH, Jang GS, Kim YJ (2004) Optimization of curcumin extraction from turmeric(Curcuma longa L.) using supercritical fluid $CO_2$. Food Eng Prog, 8, 47-52
  8. Lee KI, Choi CH, Kim SM, Pyo BS (2010) Antibacterial activity and enhancing antibiotic effect of extract and fractions from Curcuma Longa against MRSA strain. Kor J Pharmacogn, 41, 38-42
  9. Jeong SH, Jang GS, Go GH. (2004) Physiological effects of curcumin extracted by supercritical fluid from turmeric (Curcuma longa L.). Kor J Food Sci Technol, 36, 317-320
  10. Choi HY (2009) Antimicrobial activity of UIGeum (Curcuma longa L.) extract and its microbiological and sensory characteristic effects in processed foods. Kor J Food Cookery Sci, 25, 350-356
  11. Kim KY, Michael DP, Chung H.J (2000) Antimicrobial effectiveness of pine needle extracts on food borne illness bacteria. J Microbial Biotechnol, 10, 227-232.
  12. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1744
  13. Joslyn MA. (1970) Methods in food analysis. Acad press, New York, USA, p 710-711.
  14. Lee SH, Kang KM, Park HJ, Baek LM (2009) Physiological characteristics of medicinal plant for use functional materials in seasoning sauce for pork meat. Kor J Food Sci Technol, 41, 100-105
  15. Park YJ, Park YS, Towantakavanit K, Park JO, Kim YM, Jung KJ, Cho JY, Lee KD, Heo BG (2009) Chemical components an biological activity of Stauntonia hexaphylla. Kor J Plant Res, 22, 403-411
  16. Guo DJ, Cheng HL, Yu PH (2008) Antioxidative activities and total phenolic contents of tonic Chinese medicinal herbs. Inflammopharmacology, 16, 201-207 https://doi.org/10.1007/s10787-008-8016-9
  17. Cowan MM (1999) Plant products as antimicrobial agents. Clin. Microbiol Rev, 12, 564-582
  18. Cichewicz RH, Thorpe PA (1996) The antimicrobial properties of chile peppers(Capsicum species) and their uses in Mayan medicine. J Ethnopharmacol, 52, 61-70. https://doi.org/10.1016/0378-8741(96)01384-0
  19. Lambert RJW, Skandamis PN, Coote PJ and Nychas GJE (2001) A study of the mnimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol, 91, 453-562 https://doi.org/10.1046/j.1365-2672.2001.01428.x
  20. Hoult JRS, Paya M (1996) Pharmacological and biochemical actions of simple coumarins; matural products with therapeutic potential. Gen Pharmacol, 27, 713-722 https://doi.org/10.1016/0306-3623(95)02112-4
  21. Mabry TJ, Markham KR, Tomas MB (1970) The systematic identification of flavonoids. J Mole Str, 10, 320
  22. Yang MS, Ha YL, Nam SH, Choi SU, Jang DS (1995) Screening of domestic plant with antibacterial activity. Agric Chem Biotech, 38, 584-589
  23. Ahn BY (1992) Antimicrobial activity of the essential oils of Artemisia princeps var. orientalis. Kor J Food Hygiene, 7, 157-160
  24. Song YE, Ryu JS, Chung JR, Kwak JS, Kim DH, Kim BS, Rim CW (2001) Studies on the biological activity of Artemisia iwayomogi Kitamura. Kor J Med Crop Sci, 9, 116-123
  25. Lee CK, Seo JJ (2003) Antimicrobial activity of the aerial part of Artemisia capillaris extracts on the food-borne pathogens. J Kor Soc Food Sci Nutr, 32, 1227-1232 https://doi.org/10.3746/jkfn.2003.32.8.1227
  26. Singh G, Kapoor IPS, Singh P, Heluani CS, Lampasona MP, Catalan CAN (2008) Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zinger officinale. Food Chemical Toxicol, 46, 3295-3302 https://doi.org/10.1016/j.fct.2008.07.017
  27. Seo SK, Huh CK, Kim YD (2008) Changes of biologically active components in Prunus mume fruit Kor J Food Preserv, 15, 269-273
  28. Lee KH, Choi CH, Kim SM, Pyo BS (2010) Antibacterial activity and enhancing antibiotic effect of extract and fractions from Curcuma longa against MRSA strain. Kor J Pharmacogn, 41, 38-42
  29. Harman D (1987) The free radical theory of aging. In modern biological theories of aging. Raven Press, NY, p 40-80
  30. Choi HS (2006) A study on the biological activities of Achyranthis Radix ethanol extract. Chosun Univ, p 22-30, 53-59
  31. Kang YH, Park YK, Oh SR, Moon KD. (1995) Studies on the physiological functionality of pine meddle and mugwort extracts. Kor J Food Sci Technol, 27, 978-984

Cited by

  1. Physiological Activity of Acai Berry (Euterpe oleracea Mart.) Extracted with Different Solvents vol.27, pp.1, 2012, https://doi.org/10.7318/KJFC.2012.27.1.075
  2. Antioxidant and Antimicrobial Activities of Smilax china Leaf Extracts vol.18, pp.5, 2011, https://doi.org/10.11002/kjfp.2011.18.5.764
  3. Phytopreventive antihypercholesterolmic and antilipidemic perspectives of zedoary (Curcuma Zedoaria Roscoe.) herbal tea vol.15, pp.1, 2016, https://doi.org/10.1186/s12944-016-0210-y
  4. Dyeing Property and Functionality of Curcumae longae RhizomaExtracts with Different Solvents vol.26, pp.1, 2017, https://doi.org/10.5934/kjhe.2017.26.1.49
  5. Antimicrobial activities of Lindera obtusiloba Blume and Zanthoxylum piperitum DC extracts vol.21, pp.3, 2014, https://doi.org/10.11002/kjfp.2014.21.3.427
  6. Immunomodulatory Effects of Fermented Curcuma longa L. Extracts on RAW 264.7 Cells vol.43, pp.2, 2014, https://doi.org/10.3746/jkfn.2014.43.2.216
  7. Antioxidant activity and antimicrobial activity of the grasshopper, Oxya chinensis sinuosa vol.53, pp.2, 2015, https://doi.org/10.7852/jses.2015.53.2.130
  8. The effect of Gihyeolgwanjeolbang-A(CWS-A) on adjuvant-induced arthritis in rats vol.27, pp.1, 2012, https://doi.org/10.6116/kjh.2012.27.1.1
  9. Fermentation Properties and Inflammatory Cytokines Modulating of Fermented Milk with Curcuma longa L Powder vol.25, pp.1, 2015, https://doi.org/10.5352/JLS.2015.25.1.75
  10. Antioxidant and antimicrobial activities of black Doraji (Platycodon grandiflorum) vol.20, pp.4, 2013, https://doi.org/10.11002/kjfp.2013.20.4.510
  11. Antioxidant and Antimicrobial Activities of Curcuma aromatica Salisb. with and without Fermentation vol.32, pp.3, 2016, https://doi.org/10.9724/kfcs.2016.32.3.299
  12. Antimicrobial and Antioxidant Activities in Different Parts and Cultivars of Broccoli vol.32, pp.3, 2014, https://doi.org/10.7235/hort.2014.13183
  13. In Vitro Hepatoprotective Effects of Fermented Curcuma longa L. by Aspergillus oryzae against Alcohol-Induced Oxidative Stress vol.45, pp.6, 2016, https://doi.org/10.3746/jkfn.2016.45.6.812
  14. 자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색 vol.44, pp.3, 2011, https://doi.org/10.9721/kjfst.2012.44.3.337
  15. 강황을 첨가한 팬 프라잉 화전의 품질 특성과 산화방지활성 vol.49, pp.3, 2011, https://doi.org/10.9721/kjfst.2017.49.3.296
  16. 조류 인플루엔자 예방 및 면역 증진을 위한 천연 사료 첨가제 특허동향 분석 vol.18, pp.3, 2011, https://doi.org/10.5392/jkca.2018.18.03.062
  17. 붉나무 열매 추출물의 항균효능 평가에 관한 연구 vol.37, pp.1, 2011, https://doi.org/10.12925/jkocs.2020.37.1.145
  18. Antioxidant Activities of Jeju Wax Apple (Syzygium samarangense) and Safety of Human Keratinocytes and Primary Skin Irritation Test vol.7, pp.2, 2011, https://doi.org/10.3390/cosmetics7020039
  19. Antimicrobial activity and stability of Prunus mume fruit extract against Streptococcus mutans KCCM 40105 strain vol.28, pp.3, 2011, https://doi.org/10.11002/kjfp.2021.28.3.426