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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antibacterial Activity of Fermented Korean Medicine Against Multi-drug Resistant Pseudomonas aeruginosa

발효한약의 항생제 다제내성균 Pseudomonas aeruginosa를 억제하는 항균활성

  • Ryu, Ji-Yeon (Department of Laboratory Medicine, Daegu Catholic University) ;
  • Park, Young-Ja (Department of Clinical Pathology, Sorabol College) ;
  • Kim, Hyun-Soo (Department of Microbiology, College of Natural Science, Keimyung University)
  • 류지연 (대구가톨릭대학교병원 진단검사의학과) ;
  • 박영자 (서라벌대학 임상병리과) ;
  • 김현수 (계명대학교 자연과학대학 미생물학과)
  • Received : 2011.10.06
  • Accepted : 2011.12.11
  • Published : 2011.12.31
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Abstract

Bacterial antibiotic resistance is a real and growing problem for both Gram positive and Gram negative bacterial pathogens in the hospital setting. Among Gram negative bacteria, the ubiquitous bacterium Pseudomonas aeruginosa is a particular concern in immunocompromised and burn patients. The present study evaluated antibacterial activity and efficacy of a Korean herbal medicine against eight multi-drug resistant clinical isolates of P. aeruginosa (0225, 0254, 0347, 0826, 1113, 1378, 1731, and 2492) isolated at Daegu Catholic University Hospital. Methanol extracts of Galla rhois (5 and 10 mg/mL) displayed inhibition diameters for isolate 2492 of 10 and 12 mm, respectively, in a conventional disc diffusion assay. In seven kinds of Korean herbal medicines, increased inhibitory power of Lonicera japonica, Gardenia jasminoides, Galla rhois, and Scultellaria baicalensis was evident with the fermentation of six kinds of lactic acid bacteria. Three lactic acid bacteria (Lactobacillus plantarum subsp. plantarum KCTC 3108, L. casei KCTC 3109, and L. fermentum KCTC 3112) were identified as excellent strains for the production of antibacterial materials. In the six Korean herbal medicine extracts, strong inhibitory activity of fermented Forsythia suspensa, Glycyrrhizae radix, Lycium chinense, Platycodon grum, and Schizandra chinensis with five kinds of lactic acid bacteria was evident for seven multi-drug resistant P. aeruginosa isolates.

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References

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