KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and Characteristics of Alginate lyase Producing Microorganism: Sanguibacter keddieii NC9

Alginate lyase 생산 균주의 분리 및 특성: Sanguibacter keddieii NC9

  • Kim, Geun-Hyub (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Lee, Sung-Mok (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Choi, Soo-Jeong (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
  • 김근협 (신라대학교 의생명과학대 생명공학과) ;
  • 이성목 (신라대학교 의생명과학대 생명공학과) ;
  • 최수정 (신라대학교 의생명과학대 생명공학과) ;
  • 이재화 (신라대학교 의생명과학대 생명공학과)
  • Received : 2011.11.14
  • Accepted : 2011.11.29
  • Published : 2011.12.31
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Abstract

The research was purposed production of oligosaccharide from alginate hydrolysis the main composition in cell walls of sea weed. We was isolated 252 strains from sea water and mud flat, the highest alginate lyase activity was selected, and identified as Sanguibacter keddieii NC9 by 16S rDNA sequence analysis. In this study was select the sodium alginate concentration, pH, temperature for the production of alginate lyase activity. Alginate lyase activity was confirmed from plate assay with 10% cetylpyridinium chloride. The optimum culture conditions for the production of alginate lyase were sodium alginate 10 g/L, peptone 5 g/L, $40^{\circ}C$, pH 9 and 36 hours incubation time. Sanguibacter keddieii NC9, its alginate lyase would be useful for the production of bioenergy and biofunctional oligosaccharides from sea weed.

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References

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