KSBB Journal

  • 제26권6호
  • /
  • Pages.529-532
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  • 2011
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  • 1225-7117(pISSN)
  • /
  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
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Lactobacillus crispatus 생균 생산을 위한 whey 배지 최적화

Production of Viable Lactobacillus crispatus by Using Whey Based Medium

  • 장정은 (인하대학교 생명화학공학부) ;
  • 구자룡 (인하대학교 생명화학공학부) ;
  • 소재성 (인하대학교 생명화학공학부) ;
  • 윤현식 (인하대학교 생명화학공학부)
  • 투고 : 2011.08.23
  • 심사 : 2011.12.20
  • 발행 : 2011.12.31
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초록

Whey based medium was optimized for the production of viable Lactobacillus crispatus KLB 46 isolated from the vagina of Korean women. Among the various nitrogen sources such as yeast extract, beef extract, and proteose peptone no. 3 supplemented to whey, beef extract showed the highest viable cell production. The addition of Tween 80 to the whey based medium increased viable cell concentration. As beef extract supplementation is not economically attractive, corn steep liquor was added as a supplementary nitrogen sources. When corn steep liquor was supplied with beef extract with the ratio 5 : 1, the viable cell count was $3.11{\times}10^9$ CFU/mL. Also, the addition of mineral salts containing sodium acetate (5 g/L), potassium phosphate dibasic (2 g/L), magnesium sulfate (0.1 g/L) and manganese sulfate (0.05 g/L) to the whey medium increased viable cell count further ($5.00{\times}10^9$ CFU/mL).

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참고문헌

  1. Eschenbach, D. A., Davick, P. R., Williams, B. L., Klebanoff, S. J., Young-Smith, K., Critchlow, C. M., and K. K. Holmes (1989) Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis. J. Clinical Microbiol. 27: 251-256.
  2. Hughes, V. L. and S. L. Hillier (1990) Microbiologic characteristics of Lactobacillus products used for colonization of the vagina. Obstet. Gynecol. 75: 244-248.
  3. Sobel, J. D. (1989) Vaginal infections in adult women. Med. Clinical North America 74: 1578-1602.
  4. Chang, C. E., S. C. Kim, J. S. So, and H. S. Yun (2001) Cultivation of Lactobacillus crispatus KLB 46 isolated from human vagina. Biotechnol. Bioprocess Eng. 6: 128-132. https://doi.org/10.1007/BF02931958
  5. Parent, D., Bossens, M., Bayot, D., Kirkpatrick, C., Graf, F., Wilkinson, F. E., and R. R. Kaisser (1996) Therapy of bacterial vaginosis using exogenously-applied Lactobacilli Acidophili and a low dose of Estriol a placebo-controlled multicentric clinical trial. Arzne-Fors/Drug Res. 46: 68-73.
  6. Lund, B., Norddahl, B., and B. Ahring (1992) Production of lactic acid from whey using hydrolysed whey protein as nitrogen source. Biotechnol. Lett. 14: 851-856. https://doi.org/10.1007/BF01029152
  7. Amrane, A. and Y. Prigent (1998) Lactic acid production rates during the different growth phases of Lactobacillus helveticus cultivated on whey supplemented with yeast extract. Biotechnol. Letters 20: 379-383. https://doi.org/10.1023/A:1005331430943
  8. Arasaratnam, V., Senthuran, A., and K. Balasubramaniam (1996) Supplementation of with glucose and different nitrogen sources for lactic acid production by Lactobacillus delbrueckii. Enz. Microbial Technol. 19: 482-486. https://doi.org/10.1016/S0141-0229(95)00147-6
  9. De Man, J. C., Rogosa, M., and M. E. Sharpe (1960) A medium for the cultivation of Lactobacilli. J. Appl. Bacteriol. 23: 130-135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.x

피인용 문헌

  1. 프로바이오틱 Lactobacillus brevis SBB07의 균체량 증가를 위한 배양 조건 최적화 vol.28, pp.5, 2018, https://doi.org/10.5352/jls.2018.28.5.577