KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Preparation of Alginate-fibroin Beads with Diverse Structures

다양한 구조를 가진 알긴산-피브로인 비드 제조

  • Lee, Jin-Sil (Dept. of BioEngineering and Technology, Kangwon National University) ;
  • Lee, Shin-Young (Dept. of BioEngineering and Technology, Kangwon National University) ;
  • Hur, Won (Dept. of BioEngineering and Technology, Kangwon National University)
  • 이진실 (강원대학교 공과대학 생물공학과) ;
  • 이신영 (강원대학교 공과대학 생물공학과) ;
  • 허원 (강원대학교 공과대학 생물공학과)
  • Received : 2011.07.12
  • Accepted : 2011.10.19
  • Published : 2011.10.31
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Abstract

Alginate bead has been supplemented with various polymers to control permeability and to enhance mechanical strength. In this report, fibroin-reinforced alginate hydrogel was prepared, in which spatial localization of fibroin molecules was investigated. Confocal laser scanning microscopy revealed that fibroin molecules formed a fibrous network in the alginate-fibroin beads, which was expected to enhance mechanical strength as same as in many composite materials. Uniaxial compression test showed that fibroin-reinforced alginate beads had increased mechanical strength only after methanol treatment that caused ${\beta}$-sheet formation among fibroin molecules. Simultaneous curing and dialysis of alginate beads were carried out to remove excesscalcium but to retain fibroin in the dialysis chamber, which fabricated beads without internal fibrous fluorescent stains. Fibroin molecules were only found beneath the surface of the beads. The fibroin-diffused shell was further processed to form a thick wall after drying or was mobilizedto the centre of the bead by methanol treatment. Accordingly, the structure analyses provide processing methods of fibroin to form a wall or center clumps, which could be applied to design controlled delivery device.

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References

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