미생물학회지 (Korean Journal of Microbiology)

  • 제47권2호
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  • Pages.110-116
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  • 2011
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Saccharomyces cerevisiae의 균사형 생장에서 이중 특이성 인산화 효소, ScKns1p의 기능 분석

Function of Dual Specificity Kinase, ScKns1, in Adhesive and Filamentous Growth of Saccharomyces cerevisiae

  • 박윤희 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 양지민 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 양소영 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 김상미 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 조영미 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 박희문 (충남대학교 생명시스템과학대학 미생물.분자생명과학과)
  • Park, Yun-Hee (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Yang, Ji-Min (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Yang, So-Young (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Sang-Mi (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Cho, Young-Mi (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Park, Hee-Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
  • 투고 : 2011.06.09
  • 심사 : 2011.06.21
  • 발행 : 2011.06.30
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초록

ScKns1는 Saccharomyces cerevisiae에서 발견되는 이중 특이성 인산화 효소이다. S288c 계열에서 $Sckns1{\Delta}$ 균주는 야생형과 차이를 보이지 않아, ScKns1의 세포 내 기능에 대해 밝혀지지 않았다. 그러나 분열 효모에서 LAMMER kinase는 세포 엉김과 균사형 생장 및 산화 스트레스와 관련된 것으로 밝혀졌다. 따라서 형태학적 변화를 관찰할 수 있는 S. cerevisiae ${\Sigma}1278b$ 균주에서 ScKNS1 결손균주($Sckns1{\Delta}$)를 제조하고 표현형 변화를 통하여 기능을 분석하였다. $Sckns1{\Delta}$ 균주는 균사형 생장을 유도하는 질소고갈 조건과 butanol 첨가 조건에서 균사형 생장의 결함을 보였으며, agar 표면의 부착생장도 감소하였다. 또한 $Sckns1{\Delta}$ 균주에 균사형 생장을 조절하는 MAPK 경로 유전자를 도입한 결과 RAS2와 STE20에 의해서는 균사형 생장 결함이 회복되지 않았으나, STE11, STE12와 TEC1에 의해서 결합이 회복되었다. 이러한 결과들은 S. cerevisiae에서도 LAMMER kinase가 MAPK 경로를 통하여 균사형 생장을 조절함을 시사한다.

In the previous study with the Saccharomyces cerevisiae S288c strains, no known function of the dual specificity kinase, ScKns1, was reported because its gene deletion did not show any noticeable phenotypic changes. Recent study with fission yeast, however, revealed the involvement of the LAMMER kinase in flocculation, filamentous growth, oxidative stress, and so on. Therefore we made Sckns1-deletion mutants with the ${\Sigma}1278b$-background, with which one can induce filamentous and adhesive growth in contrast to those of the S288c-background. The $Sckns1{\Delta}$ strains of both haploid and diploid showed defect in filamentous growth under conditions for inducing the filamentous growth such as nitrogen starvation and butanol treatment. Both kinds of the deletion mutants also showed decrease in adhesive growth on agar surface. Interestingly enough the defects of the $Sckns1{\Delta}$ strains were suppressed by the over-expression of each gene for the components of the MAPK signaling pathway such as STE11, STE12, and TEC1, respectively, but not by the upstream components, RAS2 and STE20, respectively. Although further investigations are required, these results indicate that the ScKns1 may act in place between the Ste20 and the Ste11 of the S. cerevisiae MAPK cascade.

키워드

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