Mycobiology

The Korean Society of Mycology (한국균학회)
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Trichoderma asperellum Chi42 Genes Encode Chitinase

  • Loc, Nguyen Hoang (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Quang, Hoang Tan (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Hung, Nguyen Bao (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Huy, Nguyen Duc (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Phuong, Truong Thi Bich (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Ha, Tran Thi Thu (Institute of Resources, Environment and Biotechnology, Hue University)
  • Received : 2011.07.11
  • Accepted : 2011.08.09
  • Published : 2011.09.30
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Abstract

Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.

Keywords

References

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