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The Effects of Gonjadaesungchimjoongbang on Learning Ability and Memory after Ischemic Brain Injury in Rats

허혈성 뇌손상 백서에서 공자대성침중방(孔子大聖枕中方)이 학습과 기억에 미치는 영향

  • Ryu, Su-Hyang (Department of Pediatrics, College of Oriental Medicine, Dongshin University) ;
  • Chae, Jung-Won (Department of Pediatrics, College of Oriental Medicine, Dongshin University)
  • 유수향 (동신대학교 한의과대학 소아과학교실) ;
  • 채중원 (동신대학교 한의과대학 소아과학교실)
  • Received : 2011.03.24
  • Accepted : 2011.04.22
  • Published : 2011.04.30

Abstract

Objectives: The purpose of this study is to evaluate the effect of Gonjadaesungchimjoongbang on spatial learning abilities and memories in ischemic brain injury. Methods: Rats were separated into three groups; (1) Normal, (2) Saline medication after ischemic brain injuries (control), (3) Gonjadaesungchimjoongbang medication after ischemic brain injuries (experiment). Ischemic brain injuries was induced by MCA occlusion and reperfusion. Morris water maze test was conducted for spatial learning and memory tests. Then, the change of BDNF in the hippocampus($7^{th}$, $14^{th}$ day) was examined by immunohistoche- mistry. Results: In Morris water maze test, spatial learning abilities and memory functioning were considerably increased in the experiment group as oppose to control group on $7^{th}$ and $14^{th}$ day(p<0.01). Moreover, immunohistochemistric response of BDNF in the hippocampus indicated that the more increased immune reaction was found in the experiment group as oppose to the control group on $7^{th}$ and $14^{th}$ day. Conclusions: Gonjadaesungchimjoongbang can improve the learning abilities and memories in ischemic brain injury.

Keywords

References

  1. 박찬웅. 뇌, 학습과 기억의 구조. 서울:서울대학교 출판부. 1998:1-5.
  2. 장동환 외 2인. 심리학 입문. 서울:박영사. 1991:214,267,272,313.
  3. Eriksson PS, Perfilieva E, Bjork-Eriksson T, Alborn AM, Nordborg C, Peterson DA, Gage FH. Neurogenesis in the adult human hippocampus. Nat. Med. 1998;4:1313-7. https://doi.org/10.1038/3305
  4. Shors TJ, Miesegaes G, Beylin A, Zhao M, Rydel T, Gould E. Neurogenesis in the adult is involved in the formation of trace memories. Nature. 2001;410:372-6. https://doi.org/10.1038/35066584
  5. Connor B, Dragunow M. The role of neuronal growth factors in neurodegenerative disorders of the human brain. Brain Res Reviews. 1998;27(1):1-39. https://doi.org/10.1016/S0165-0173(98)00004-6
  6. Levine E, Dreyfus C, Black I. Differential effects of NGF and BDNF on voltage-gated calcium currents in embryonic based vorebrain neurons. J. Neurosci. 1995;15:3084-91.
  7. Mizuno M, Yamada K, Olariu A, Nawa H, and Nabeshima T. Involvement of brain-derived neurotrophic factor in spatial memory formation and maintenance in a radial arm maze test in rats. J. Neurosci. 2000;20:7116-21.
  8. 류영수, 최공환. 기억장애에 관한 동서의학적 비교 연구. 동의신경정신과학회지. 1996;7(1):155-66.
  9. 대한한방신경정신과학회. 한방신경정신의학. 파주:집문당. 2005:233-8.
  10. 陳柱杓. 對譯萬病回春. 서울:법인문화사. 2007:519.
  11. 許浚. 東醫寶鑑. 서울:법인문화사. 2005:335-9.
  12. Longa EZ, Weinstein PR, Carison S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 1989;20(1):84-91. https://doi.org/10.1161/01.STR.20.1.84
  13. Garcia JH, Wagner S, Liu KF, Hu XJ. Neurological deficit and extent of neuronal necrosis attributable to middle cerebral artery occlusion in rats. Statistical validation. Stroke. 1995;26:627-34. https://doi.org/10.1161/01.STR.26.4.627
  14. Raymond DA, Maurice Victor, Allan HR. 아담스 신경과학. 서울:정담. 1998:715-22.
  15. 정봉교, 윤병수, 박순권. 흰쥐의 내측중경핵 손상이 Morris 수중미로과제의 학습에 미치는 효과. 한국심리학회지. 1993;5:29-44.
  16. Barde Y, Edgar D, Theonen H. Durification of a new neurotrophic factor from mammalian brain. EMBO J. 1982;1(5):549-53.
  17. Connor B, Dragunow M. The role of neuronal growth factors in neurodegenerative disorders of the human brain. Brain Res Reviews. 1998;27(1):1-39. https://doi.org/10.1016/S0165-0173(98)00004-6
  18. 김식현, 남기원. 신경영양성 인자와 역할. 대한물리치료학회지. 1999;11(2):131-7.
  19. Lee JL, Everitt BJ, and Thomas KL. Independent cellular processes for hippocampal memory consolidation and reconsolidation. Science. 2004;304:839-43. https://doi.org/10.1126/science.1095760
  20. Bekinschtein P, Cammarota M, Katche C, Slipczuk L, Rossato JL, Goldin A, Izguierdo I and Medina JH. BDNF is essential to promote persistence of long-term memory storage. Proc. Natl. Acad. Sci. 2008;105:2711-6. https://doi.org/10.1073/pnas.0711863105
  21. Sairanen M, Lucas G, Ernfors P, Castren M and Castren E. Brain-derived neurotrophic factor and anti-depressant drugs have different but coordinated effects on neuronal turnover, proliferation, and survival in the adult denate gyrus. J. Neurosci. 2005;25:1089-94. https://doi.org/10.1523/JNEUROSCI.3741-04.2005
  22. Murray KD, Gall CM, Jones EG, and Isackson PJ. Differential regulation of brain-derived neurotrophic factor and type II calcium/calmodulin-dependent protein kinase messenger RHA expression in Alzheimer's disease. Neuroscience. 1994;60:37-48. https://doi.org/10.1016/0306-4522(94)90202-X
  23. 馬元臺, 張隱庵. 黃帝內經素問靈樞合編. 台聯:國風出版社. 1986:素問-532, 靈樞-57,58,464.
  24. 이시진. 본초강목. 중의약출판사. 1998:825.
  25. 韓醫科大學 本草學 編輯委員會. 本草學. 서울:永林社. 2004:530-1, 535-6, 565-6, 662-3.
  26. 김창민, 신민교, 이경순, 안덕균외 역. 中藥大辭典.서울:鼎談. 2001:519-22, 2306-12, 3168-72, 3305-10.
  27. 황시영, 강형원, 류영수. 원지에 대한 뇌 성상세포로부터 염증성 세포활성물질 분비의 억제 효과에 관한 연구. 동의신경정신과학회지. 1999;10(1):95-108.
  28. 박대규, 이완하. 원지 Saponin의 이뇨효과 및 중추억제작용에 관한 연구. 생약학회지. 1983;14(4):178-92.
  29. 이영종, 성낙술, 이수배. 원지가 NMDA로 유발된 신경세포 손상에 미치는 효과. 대한본초학회지. 2005;20(2):115-25.
  30. 강선태, 이태호. 어혈병태모형에 미치는 천궁, 석창포 및 반하의 효능에 관한 실험적 연구. 동의병리학회지. 1989;4:57-73.
  31. 정현우, 강성용, 백승화. 석창포가 혈압 및 국소뇌혈류량에 미치는 영향. 대한본초학회지. 1999;14(2):81-8.
  32. 박경수. 석창포 정유 향기의 흡입이 백서의 경련억제에 미치는 영향. 동국대학교대학원 학위논문. 2001:1- 47.
  33. 오찬호, 이창현. 석창포의 면역조절 및 항암효과. 동의생리병리학회지. 2007;21(4):869-73.
  34. Aronowski J, Ostrow P, Samways E, Strong R, Zivin JA & Grotta JC. Graded bioassay for demonstration of brain rescue from experimental acute ischemia in rats. Stroke. 1994;25(11):2235-40. https://doi.org/10.1161/01.STR.25.11.2235
  35. Goldlust EJ, Paczynski PP, He YY, Hsu CY & Goldberg MP. Automated measurement of infarct size with scanned images of triphenyltetrazolium chloride-stained rat brains. Stroke. 1996;27(9):1657-62. https://doi.org/10.1161/01.STR.27.9.1657
  36. Buchan AM, Pulsineli WA. Hypothermia but not the N-methyl-D-aspartate receptor antagonist, MK-801, attenuates neuronal damage in gerbils subjected to transient global ischemia. J. Neurosci. 1990;11:1049-50.
  37. Gill R, Foster A, Woodruff G. MK-801 is neuroprotective in gerbils when administered during the post-ischemic period. Neuroscience. 1988;25:847-55. https://doi.org/10.1016/0306-4522(88)90040-1
  38. Yamazaki M, Matsuoka N, Kuratani K, Ohkubo Y, Yamaguchi I. FR121196, a potential antidementia drug, ameliorates the impaired memory of rat in the Morris water maze. J. Pharmacol Exp Ther. 1995;272(1):256-63.
  39. 정봉교, 윤병수, 박순권. 흰쥐의 내측중경핵 손상이 Morris 수중미로과제의 학습에 미치는 효과. 한국심리학회지. 1993;5:29-44.