Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
권호 표지

Role of Reactive Oxygen Species in the Adipogenesis of Adipose-derived Stem Cells

지방유래줄기세포의 지방분화과정에서 활성산소가 미치는 영향

  • Chang, Hak (Research Institute of Plastic and Reconstructive Surgery, Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Min, Kyung-Hee (Department of Plastic and Reconstructive Surgery, Eulji General Hospital) ;
  • Park, Young-In (Research Institute of Plastic and Reconstructive Surgery, Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Kim, Yo-Han (Department of Plastic and Reconstructive Surgery, Eulji General Hospital) ;
  • Minn, Kyung-Won (Research Institute of Plastic and Reconstructive Surgery, Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine)
  • 장학 (서울대학교병원 성형외과학교실 성형재건연구소) ;
  • 민경희 (을지대학교 의과대학 을지병원 성형외과학교실) ;
  • 박영인 (서울대학교병원 성형외과학교실 성형재건연구소) ;
  • 김요한 (을지대학교 의과대학 을지병원 성형외과학교실) ;
  • 민경원 (서울대학교병원 성형외과학교실 성형재건연구소)
  • Received : 2010.10.21
  • Accepted : 2010.12.28
  • Published : 2011.03.10
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: Stem cells continue to receive research attention in the clinical fields, and adipose-derived stem cells (ADSCs) have been shown to be a good source raw material. Many plastic surgeons are researching the ADSC adipogenesis with a view of conducting clinical trials, and many attempts have been made to identify the factors that promote the adipogenesis of ADSCs, but comparatively few correlation studies have been undertaken to explore the relation between reactive oxygen species (ROS) and the ADSC adipogenesis. We undertook this study is to investigate the effects of ROS on ADSC adipogenesis. Methods: ADSCs were isolated and cultured from abdominal adipose tissue, and cultured in different media; 1) DMEM(control), 2) adipogenesis induction culture medium, 3) adipogenesis induction culture medium with ROS ($20{\mu}M/50{\mu}M\;H_2O_2$), 4) adipogenesis induction culture medium containing ROS ($20{\mu}M/50{\mu}M\;H_2O_2$) and antioxidant ($10{\mu}M/20{\mu}M$ Deferoxamine). We compared adipogenesis in these different media by taking absorbance measurements after Oil-Red O staining every 5 days. Results: After culturing for 20 days, significant differences were observed between these various culture groups. Absorbance results showed significantly more adipogenesis had occurred in media containing adipogenesis induction culture medium and $H_2O_2$ (in a $H_2O_2$ dose-dependently manner) than in media containing adipogenesis induction culture medium and no $H_2O_2$ (p<0.001). Furthermore, in media containing adipogenesis induction culture medium, $H_2O_2$, and antioxidant, absorbance results were significantly lower than in adipogenesis induction culture medium and $H_2O_2$ (p<0.001). Conclusion: These findings suggest that ROS promote the adipogenesis of ADSCs. We suggests that ROS could be used in the adipose tissue engineering to improve fat cell differentiation and implantable fat tissue organization.

Keywords

References

  1. Zuk PA, Zuk M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7: 211, 2001 https://doi.org/10.1089/107632701300062859
  2. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH: Human adipose tissue is a source for multipotent stem cells. Mol Biol Cell 13: 4279, 2002 https://doi.org/10.1091/mbc.E02-02-0105
  3. Hemmrich K, Van de Sijpe K, Rhodes NP, Hunt JA, Di Bartolo C, Pallua N, Blondeel P, von Heimburg D: Autologous in vivo adipose tissue engineering in hyaluronanbased gels-a pilot study. J Surg Res 144: 82, 2008 https://doi.org/10.1016/j.jss.2007.03.017
  4. Aguiari P, Leo S, Zavan B, Vindigni V, Rimessi A, Bianchi K, Franzin C, Cortivo R, Rossato M, Vettor R, Abatangelo G, Pozzan T, Pinton P, Rizzuto R: High glucose induces adipogenic differentiation of muscle-derived stem cells. Proc Natl Acad Sci USA 105: 1226, 2008 https://doi.org/10.1073/pnas.0711402105
  5. Hong L, Peptan IA, Coplan A, Daw JL: Adipose tissue engineering by human adipose-derived stem cells. Cells Tissues Organs 183: 133, 2006 https://doi.org/10.1159/000095987
  6. Greco AV, Mingrone G, Giancaterini A, Manco M, Morroni M, Cinti S, Granzotto M, Vettor R, Camastra S, Ferrannini E: Insulin resistance in morbid obesity: Reversal with intramyocellular fat depletion. Diabetes 51: 144, 2002 https://doi.org/10.2337/diabetes.51.2007.S144
  7. Shimomura I, Hammer RE, Richardson JA, Ikemoto S, Bashmakov Y, Goldstein JL, Brown MS: Insulin resistance and diabetes mellitus in transgenic mice expressing nuclear SREBP-1c in adipose tissue: model for congenital generalized lipodystrophy. Genes Dev 12: 3182, 1998 https://doi.org/10.1101/gad.12.20.3182
  8. Reitman A, Friedrich I, Ben-Amotz A, Levy Y: Low plasma antioxidants and normal plasma B vitamines and homocysteine in patients with severe obesity. Isr Med Assoc J 4: 590, 2002
  9. Fujita K, Nishizawa H, Funahashi T, Shimomura I, Shimabukuro M: Systemic oxidative stress is associated with visceral fat accumulation and the metabolic syndrome. Circ J 70: 1437, 2006 https://doi.org/10.1253/circj.70.1437
  10. Hwang JT, Park IJ, Shin JI, Lee YK, Lee SK, Baik HW, Ha J, Park OJ: Genistein, EGCG, and capsaicin inhibit adipocyte differentiation process via activating AMP-activated protein kinase. Biochem Biophys Res Commun 338: 694, 2005 https://doi.org/10.1016/j.bbrc.2005.09.195
  11. Schroder K, Wandzioch K, Helmcke I, Brandes RP: Nox4 acts as a switch between differentiationand proliferation in preadipocytes. Arterioscler Thromb Vasc Biol 29: 239, 2009 https://doi.org/10.1161/ATVBAHA.108.174219
  12. Pessler-Cohen D, Pekala PH, Kovsan J, Bloch-Damti A, Rudich A, Bashan N: GLUT4 repression in response to oxidative stress is associated with reciprocal alterations in C/EBP alpha and delta isoforms in 3T3-L1 adipocytes. Arch Physiol Biochem 112: 3, 2006 https://doi.org/10.1080/13813450500500399
  13. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J: Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44, 2007 https://doi.org/10.1016/j.biocel.2006.07.001
  14. Ma T, Grayson WL, Frohlich M, Vunjak-Novakovic G: Hypoxia and stem cell-based engineering of mesenchymal tissues. Biotechnol Prog 25: 32, 2009 https://doi.org/10.1002/btpr.128
  15. Guzy RD, Hoyos B, Robin E, Chen H, Liu L, Mansfield KD, Simon MC, Hammerling U, Schumacker PT: Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab 1: 401, 2005 https://doi.org/10.1016/j.cmet.2005.05.001